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DCTN1 (NM_001135040) Human Tagged Lenti ORF Clone

  • LentiORF®
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SKU
RC226372L4
Lenti ORF clone of Human dynactin 1 (DCTN1), transcript variant 3, mGFP tagged
ORF Plasmid:
mGFP DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $1,391.00
3 Weeks*
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Other products for "DCTN1 or NM_001135040"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DCTN1
Synonyms DAP-150; DP-150; P135
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC226372).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001135040
ORF Size 3759 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001135040.1
RefSeq ORF 3762 bp
Locus ID 1639
UniProt ID Q14203
Cytogenetics 2p13.1
Protein Families Druggable Genome
Protein Pathways Huntington's disease
MW 138.6 kDa
Summary This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA). provided by RefSeq, Oct 2008
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"NM_001135040" in other vectors (4)
SKU Description Size Price
RC226372 DCTN1 (Myc-DDK-tagged)-Human dynactin 1 (DCTN1), transcript variant 3 10 ug
$1,091.00
RC226372L3 Lenti ORF clone of Human dynactin 1 (DCTN1), transcript variant 3, Myc-DDK-tagged 10 ug
$1,391.00
RG226372 DCTN1 (tGFP-tagged) - Human dynactin 1 (DCTN1), transcript variant 3 10 ug
$1,291.00
SC326364 DCTN1 (untagged)-Human dynactin 1 (DCTN1), transcript variant 3 10 ug
$1,201.00

Plasmid Map for RC226372L4

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Cloning scheme for RC226372L4
Circular map for RC226372L4

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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