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Ghrelin Receptor (GHSR) (NM_004122) Human Tagged Lenti ORF Clone

  • LentiORF®
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SKU
RC223879L2
Lenti ORF clone of Human growth hormone secretagogue receptor (GHSR), transcript variant 1b, mGFP tagged
ORF Plasmid:
mGFP DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $600.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Ghrelin Receptor
Synonyms GHDP
Vector pLenti-C-mGFP
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC223879).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004122
ORF Size 867 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004122.1
RefSeq Size 914 bp
RefSeq ORF 870 bp
Locus ID 2693
UniProt ID Q92847
Cytogenetics 3q26.31
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Neuroactive ligand-receptor interaction
MW 32.2 kDa
Summary This gene encodes a member of the G-protein coupled receptor family. The encoded protein may play a role in energy homeostasis and regulation of body weight. Two identified transcript variants are expressed in several tissues and are evolutionary conserved in fish and swine. One transcript, 1a, excises an intron and encodes the functional protein; this protein is the receptor for the Ghrelin ligand and defines a neuroendocrine pathway for growth hormone release. The second transcript (1b) retains the intron and does not function as a receptor for Ghrelin; however, it may function to attenuate activity of isoform 1a. Mutations in this gene are associated with autosomal idiopathic short stature.provided by RefSeq, Apr 2010
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"NM_004122" in other vectors (6)
SKU Description Size Price
RC223879 GHSR (Myc-DDK-tagged)-Human growth hormone secretagogue receptor (GHSR), transcript variant 1b 10 ug
$300.00
RC223879L1 Lenti ORF clone of Human growth hormone secretagogue receptor (GHSR), transcript variant 1b, Myc-DDK-tagged 10 ug
$600.00
RC223879L3 Lenti ORF clone of Human growth hormone secretagogue receptor (GHSR), transcript variant 1b, Myc-DDK-tagged 10 ug
$600.00
RC223879L4 Lenti ORF clone of Human growth hormone secretagogue receptor (GHSR), transcript variant 1b, mGFP tagged 10 ug
$600.00
RG223879 GHSR (tGFP-tagged) - Human growth hormone secretagogue receptor (GHSR), transcript variant 1b 10 ug
$489.00 $500.00
SC128078 GHSR (untagged)-Human growth hormone secretagogue receptor (GHSR), transcript variant 1b 10 ug
$300.00

Plasmid Map for RC223879L2

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Cloning scheme for RC223879L2
Circular map for RC223879L2

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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