CD151 (NM_139029) Human Tagged Lenti ORF Clone

  • LentiORF®
SKU
RC219655L4
Lenti ORF clone of Human CD151 molecule (Raph blood group) (CD151), transcript variant 4, mGFP tagged
  $600.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CD151
Synonyms GP27; MER2; PETA-3; RAPH; SFA1; TSPAN24
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC219655).
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_139029
ORF Size 759 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_139029.1, NP_620598.1
RefSeq Size 1570 bp
RefSeq ORF 762 bp
Locus ID 977
UniProt ID P48509
Cytogenetics 11p15.5
Protein Families Druggable Genome, Transmembrane
MW 28.3 kDa
Summary The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It is involved in cellular processes including cell adhesion and may regulate integrin trafficking and/or function. This protein enhances cell motility, invasion and metastasis of cancer cells. Multiple alternatively spliced transcript variants that encode the same protein have been described for this gene. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_139029" in other vectors (4)
SKU Description Size Price
RC219655 CD151 (Myc-DDK-tagged)-Human CD151 molecule (Raph blood group) (CD151), transcript variant 4 10 ug
$289.00 $300.00
RC219655L3 Lenti ORF clone of Human CD151 molecule (Raph blood group) (CD151), transcript variant 4, Myc-DDK-tagged 10 ug
$600.00
RG219655 CD151 (tGFP-tagged) - Human CD151 molecule (Raph blood group) (CD151), transcript variant 4 10 ug
$500.00
SC310704 CD151 (untagged)-Human CD151 molecule (Raph blood group) (CD151), transcript variant 4 10 ug
$330.00

Plasmid Map for RC219655L4

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Cloning scheme for RC219655L4

Circular map for RC219655L4

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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