MAST1 (NM_014975) Human Tagged Lenti ORF Clone

  • LentiORF®
SKU
RC215419L2
Lenti ORF clone of Human microtubule associated serine/threonine kinase 1 (MAST1), mGFP tagged
  $1,643.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MAST1
Synonyms MCCCHCM; SAST
Vector pLenti-C-mGFP
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC215419).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014975
ORF Size 4710 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014975.1, NP_055790.1
RefSeq Size 4833 bp
RefSeq ORF 4713 bp
Locus ID 22983
UniProt ID Q9Y2H9
Cytogenetics 19p13.13
Protein Families Druggable Genome, Protein Kinase
MW 170.5 kDa
Summary This gene is a member of the microtubule-associated serine/threonine kinase (MAST) family. The protein encoded by this gene has an N-terminal serine/threonine kinase domain followed by a postsynaptic density protein-95/discs large/zona occludens-1 (PDZ) domain. In mouse and rat, the orthologous protein associates with the cytoskeleton and can bind both beta-2-syntrophin and neuronal nitric oxide synthase (nNOS) through its PDZ domain. In mouse and rat, this protein also co-localizes with dystrophin- and utrophin-associated protein complexes (DAPC/UAPC) in the vascular endothelium of the central nervous system. provided by RefSeq, May 2017
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Documents
Resources
"NM_014975" in other vectors (6)
SKU Description Size Price
RC215419 MAST1 (Myc-DDK-tagged)-Human microtubule associated serine/threonine kinase 1 (MAST1) 10 ug
$1,343.00
RC215419L1 Lenti ORF clone of Human microtubule associated serine/threonine kinase 1 (MAST1), Myc-DDK-tagged 10 ug
$1,643.00
RC215419L3 Lenti ORF clone of Human microtubule associated serine/threonine kinase 1 (MAST1), Myc-DDK-tagged 10 ug
$1,643.00
RC215419L4 Lenti ORF clone of Human microtubule associated serine/threonine kinase 1 (MAST1), mGFP tagged 10 ug
$1,643.00
RG215419 MAST1 (tGFP-tagged) - Human microtubule associated serine/threonine kinase 1 (MAST1) 10 ug
$1,543.00
SC304190 MAST1 (untagged)-Human microtubule associated serine/threonine kinase 1 (MAST1) 10 ug
$1,478.00

Plasmid Map for RC215419L2

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Cloning scheme for RC215419L2

Circular map for RC215419L2

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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