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EPO (NM_000799) Human Tagged Lenti ORF Clone

  • LentiORF®
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SKU
RC210775L4
Lenti ORF clone of Human erythropoietin (EPO), mGFP tagged
ORF Plasmid:
mGFP DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $750.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol EPO
Synonyms DBAL; ECYT5; EP; MVCD2
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC210775).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_000799
ORF Size 579 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_000799.2
RefSeq Size 1340 bp
RefSeq ORF 582 bp
Locus ID 2056
UniProt ID P01588
Cytogenetics 7q22.1
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
Protein Pathways Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Jak-STAT signaling pathway
MW 21.31 kDa
Summary This gene encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The encoded protein is mainly synthesized in the kidney, secreted into the blood plasma, and binds to the erythropoietin receptor to promote red blood cell production, or erythropoiesis, in the bone marrow. Expression of this gene is upregulated under hypoxic conditions, in turn leading to increased erythropoiesis and enhanced oxygen-carrying capacity of the blood. Expression of this gene has also been observed in brain and in the eye, and elevated expression levels have been observed in diabetic retinopathy and ocular hypertension. Recombinant forms of the encoded protein exhibit neuroprotective activity against a variety of potential brain injuries, as well as antiapoptotic functions in several tissue types, and have been used in the treatment of anemia and to enhance the efficacy of cancer therapies. provided by RefSeq, Aug 2017
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"NM_000799" in other vectors (6)
SKU Description Size Price
RC210775 EPO (Myc-DDK-tagged)-Human erythropoietin (EPO) 10 ug
$450.00
RC210775L1 Lenti ORF clone of Human erythropoietin (EPO), Myc-DDK-tagged 10 ug
$750.00
RC210775L2 Lenti ORF clone of Human erythropoietin (EPO), mGFP tagged 10 ug
$750.00
RC210775L3 Lenti ORF clone of Human erythropoietin (EPO), Myc-DDK-tagged 10 ug
$750.00
RG210775 EPO (tGFP-tagged) - Human erythropoietin (EPO) 10 ug
$650.00
SC125341 EPO (untagged)-Human erythropoietin (EPO) 10 ug
$300.00

Plasmid Map for RC210775L4

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Cloning scheme for RC210775L4
Circular map for RC210775L4

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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