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Glutaminase (GLS) (NM_014905) Human Tagged Lenti ORF Clone

  • LentiORF®
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SKU
RC206265L2
Lenti ORF clone of Human glutaminase (GLS), nuclear gene encoding mitochondrial protein, mGFP tagged
ORF Plasmid:
mGFP DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $1,219.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Glutaminase
Synonyms AAD20; CASGID; DEE71; EIEE71; GAC; GAM; GDPAG; GLS1; KGA
Vector pLenti-C-mGFP
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC206265).
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_014905
ORF Size 2007 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014905.2
RefSeq Size 4799 bp
RefSeq ORF 2010 bp
Locus ID 2744
UniProt ID O94925
Cytogenetics 2q32.2
Domains ANK, Glutaminase
Protein Pathways Alanine, Arginine and proline metabolism, aspartate and glutamate metabolism, D-Glutamine and D-glutamate metabolism, Metabolic pathways, Nitrogen metabolism
MW 73.3 kDa
Summary This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants. provided by RefSeq, Jan 2012
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"NM_014905" in other vectors (6)
SKU Description Size Price
RC206265 GLS (Myc-DDK-tagged)-Human glutaminase (GLS), nuclear gene encoding mitochondrial protein 10 ug
$919.00
RC206265L1 Lenti ORF clone of Human glutaminase (GLS), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
$1,219.00
RC206265L3 Lenti ORF clone of Human glutaminase (GLS), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged 10 ug
$1,219.00
RC206265L4 Lenti ORF clone of Human glutaminase (GLS), nuclear gene encoding mitochondrial protein, mGFP tagged 10 ug
$1,219.00
RG206265 GLS (tGFP-tagged) - Human glutaminase (GLS), nuclear gene encoding mitochondrial protein 10 ug
$789.00 $1,119.00
SC114750 GLS (untagged)-Human glutaminase (GLS), nuclear gene encoding mitochondrial protein 10 ug
$920.00

Plasmid Map for RC206265L2

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file-download TXT (FASTA format)

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Cloning scheme for RC206265L2
Circular map for RC206265L2

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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