EHD2 (NM_014601) Human Tagged Lenti ORF Clone

  • LentiORF®
SKU
RC204848L4
Lenti ORF clone of Human EH-domain containing 2 (EHD2), mGFP tagged
  $805.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol EHD2
Synonyms PAST2
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC204848).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014601
ORF Size 1629 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014601.2, NP_055416.2
RefSeq Size 3601 bp
RefSeq ORF 1632 bp
Locus ID 30846
UniProt ID Q9NZN4
Cytogenetics 19q13.33
Protein Pathways Endocytosis
MW 61.2 kDa
Summary This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_014601" in other vectors (6)
SKU Description Size Price
RC204848 EHD2 (Myc-DDK-tagged)-Human EH-domain containing 2 (EHD2) 10 ug
$505.00
RC204848L1 Lenti ORF clone of Human EH-domain containing 2 (EHD2), Myc-DDK-tagged 10 ug
$805.00
RC204848L2 Lenti ORF clone of Human EH-domain containing 2 (EHD2), mGFP tagged 10 ug
$805.00
RC204848L3 Lenti ORF clone of Human EH-domain containing 2 (EHD2), Myc-DDK-tagged 10 ug
$805.00
RG204848 EHD2 (tGFP-tagged) - Human EH-domain containing 2 (EHD2) 10 ug
$489.00 $705.00
SC319541 EHD2 (untagged)-Human EH-domain containing 2 (EHD2) 10 ug
$506.00

Plasmid Map for RC204848L4

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Cloning scheme for RC204848L4

Circular map for RC204848L4

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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