RPP14 (NM_007042) Human Tagged Lenti ORF Clone

  • LentiORF®
SKU
RC204469L4
Lenti ORF clone of Human ribonuclease P/MRP 14kDa subunit (RPP14), transcript variant 2, mGFP tagged
  $450.00
3 Weeks*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RPP14
Synonyms P14
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(RC204469).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007042
ORF Size 372 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007042.1
RefSeq Size 3266 bp
RefSeq ORF 375 bp
Locus ID 11102
UniProt ID O95059
Cytogenetics 3p14.3
Domains MaoC_dehydratas
MW 13.7 kDa
Summary This gene encodes a subunit of ribonuclease P and has 3' to 5' exoribonuclease activity. Transcripts for this gene are bicistronic and include a conserved downstream open reading frame for the hydroxyacyl-thioester dehydratase type 2 (HTD2) gene. provided by RefSeq, May 2017
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Documents
Resources
"NM_007042" in other vectors (4)
SKU Description Size Price
RC204469 RPP14 (Myc-DDK-tagged)-Human ribonuclease P/MRP 14kDa subunit (RPP14), transcript variant 2 10 ug
$150.00
RC204469L3 Lenti ORF clone of Human ribonuclease P/MRP 14kDa subunit (RPP14), transcript variant 2, Myc-DDK-tagged 10 ug
$450.00
RG204469 RPP14 (tGFP-tagged) - Human ribonuclease P/MRP 14kDa subunit (RPP14), transcript variant 2 10 ug
$489.00
SC115755 RPP14 (untagged)-Human ribonuclease P/MRP 14kDa subunit (RPP14), transcript variant 2 10 ug
$150.00

Plasmid Map for RC204469L4

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Cloning scheme for RC204469L4

Circular map for RC204469L4

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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