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Pdcd1 (NM_008798) Mouse Tagged Lenti ORF Clone

  • LentiORF®
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SKU
MR227347L2
Lenti ORF clone of Pdcd1 (mGFP-tagged) - Mouse programmed cell death 1 (Pdcd1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
mGFP tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $750.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Pdcd1 or NM_008798"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pdcd1
Synonyms Ly101; PD-1; Pdc1
Vector pLenti-C-mGFP
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection None
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(MR227347).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008798
ORF Size 867 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008798.2, NP_032824.1
RefSeq Size 1972 bp
RefSeq ORF 867 bp
Locus ID 18566
UniProt ID Q02242
Cytogenetics 1 D
Summary Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PubMed:10485649, PubMed:11698646, PubMed:11209085, PubMed:21300912). Delivers inhibitory signals upon binding to ligands, such as CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:11015443, PubMed:11224527, PubMed:22641383, PubMed:18287011, PubMed:18641123). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (PubMed:22641383). Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta (PubMed:11698646, PubMed:22641383). The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (By similarity).UniProtKB/Swiss-Prot Function
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"NM_008798" in other vectors (6)
SKU Description Size Price
MC209155 PD-1 / PDCD1 (untagged) - Mouse programmed cell death 1 (PD-1 / PDCD1), (10ug) 10 ug
$450.00
MG227347 PD-1 / PDCD1 (tGFP-tagged) - Mouse programmed cell death 1 (PD-1 / PDCD1) 10 ug
$650.00
MR227347 PD-1 / PDCD1 (Myc-DDK-tagged) - Mouse programmed cell death 1 (PD-1 / PDCD1) 10 ug
$450.00
MR227347L1 Lenti ORF clone of Pdcd1 (Myc-DDK-tagged) - Mouse programmed cell death 1 (Pdcd1) 10 ug
$750.00
MR227347L3 Lenti ORF clone of Pdcd1 (Myc-DDK-tagged) - Mouse programmed cell death 1 (Pdcd1) 10 ug
$750.00
MR227347L4 Lenti ORF clone of Pdcd1 (mGFP-tagged) - Mouse programmed cell death 1 (Pdcd1) 10 ug
$750.00

Plasmid Map for MR227347L2

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file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR227347L2
Circular map for MR227347L2

file-alt Citations

PD-1 mediates microglia polarization via the MAPK signaling pathway to protect blood-brain barrier function during cerebral ischemia/reperfusion
Huang, Li, Li et al
Brain Res Bull (2024) 216, 111055 DOI: 10.1016/j.brainresbull.2024.111055
Cited by 13
AI Summary: OriGene's PD-1 overexpression mGFP-tagged lentivector particles (#MR227347L2 V) were used to transfect cells in the in vitro model, enabling the study of PD-1's role in microglia polarization and blood-brain barrier protection during cerebral ischemia/reperfusion.
Snippet: Cells in the OGD model group were transfected with PD-1 overexpression mGFP-tagged lentivector particles (#MR227347L2 V, Origene;OGD+O-PD1) or the empty vector (OGD+O-NC) by using Lipofectamine 2000 Reagent (Invitrogen, Carlsbad, CA, USA)
Blockade of Tumor-Expressed PD-1 promotes lung cancer growth
Du, McCall, Park et al
Oncoimmunology (2018) 7 (4), e1408747 DOI: 10.1080/2162402X.2017.1408747
AI Summary: OriGene products, specifically the Human PDCD1 (PD-1) cDNA clone and anti-PD-1 antibody, were used in this study to detect and manipulate PD-1 expression in lung cancer cells, enabling the authors to demonstrate that tumor-intrinsic PD-1 can regulate cancer cell growth and that its blockade may paradoxically promote tumor progression.
FigureFigureFigure
Blockade of Tumor-Expressed PD-1 Promotes Lung Cancer Growth
Du, McCall, Park et al
OncoImmunology (2017)
AI Summary: OriGene contributed to this study by providing PD-1 overexpression mGFP-tagged lentivector particles (#MR227347L2V) and polybrene, which were used to generate PD-1-overexpressing lung cancer cells for experimental analysis.
Snippet: PD - 1 - OE cells were generated by transfecting M109 cells with a PD - 1 - overexpression mGFP - tagged lentivector particles purcha sed from O rigene ( # MR227347L2 ░ V) according to the manufacturer’s protocol using polybrene (Origene) to enhance transfection
Showing 1-3 of 3 papers.
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