Jade2 (NM_199299) Mouse Tagged Lenti ORF Clone

  • LentiORF®
SKU
MR224461L4
Lenti ORF clone of Phf15 (mGFP-tagged) - Mouse PHD finger protein 15 (Phf15)
  $1,059.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Jade2
Synonyms 1200017K05Rik; AI480685; mKIAA0239; Phf15
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(MR224461).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_199299
ORF Size 2487 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_199299.3
RefSeq Size 6100 bp
RefSeq ORF 2490 bp
Locus ID 76901
UniProt ID Q6ZQF7
Cytogenetics 11 B1.3
Summary Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo (By similarity). Acts as a E3 ubiquitin-protein ligase mediating the ubiquitination and subsequent proteasomal degradation of target protein histone demethylase KDM1A. Also acts as a ubiquitin ligase E3 toward itself. Positive regulator of neurogenesis (PubMed:25018020).UniProtKB/Swiss-Prot Function
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"NM_199299" in other vectors (4)
SKU Description Size Price
MC221953 Phf15 (untagged) - Mouse PHD finger protein 15 (Phf15), (10ug) 10 ug
$836.00
MG224461 Phf15 (tGFP-tagged) - Mouse PHD finger protein 15 (Phf15), (10ug) 10 ug
$959.00
MR224461 Phf15 (Myc-DDK-tagged) - Mouse PHD finger protein 15 (Phf15) 10 ug
$589.00 $759.00
MR224461L3 Lenti ORF clone of Phf15 (Myc-DDK-tagged) - Mouse PHD finger protein 15 (Phf15) 10 ug
$1,059.00

Plasmid Map for MR224461L4

Download sequence for

Cloning scheme for MR224461L4

Circular map for MR224461L4

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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