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Slc8a3 (NM_080440) Mouse Tagged Lenti ORF Clone

  • LentiORF®
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SKU
MR221680L4
Lenti ORF clone of Slc8a3 (mGFP-tagged) - Mouse solute carrier family 8 (sodium/calcium exchanger), member 3 (Slc8a3), transcript variant 2
Interest in protein/lysate? Submit request here!
ORF Plasmid:
mGFP tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $1,150.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Slc8a3
Synonyms AW742262; Ncx3
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(MR221680).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_080440
ORF Size 2784 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_080440.3, NP_536688.2
RefSeq Size 4964 bp
RefSeq ORF 2787 bp
Locus ID 110893
UniProt ID S4R2P9
Cytogenetics 12 37.44 cM
Summary Mediates the electrogenic exchange of Ca(2+) against Na(+) ions across the cell membrane, and thereby contributes to the regulation of cytoplasmic Ca(2+) levels and Ca(2+)-dependent cellular processes. Contributes to cellular Ca(2+) homeostasis in excitable cells, both in muscle and in brain (PubMed:14722618, PubMed:21593315). In a first phase, voltage-gated channels mediate the rapid increase of cytoplasmic Ca(2+) levels due to release of Ca(2+) stores from the endoplasmic reticulum. SLC8A3 mediates the export of Ca(2+) from the cell during the next phase, so that cytoplasmic Ca(2+) levels rapidly return to baseline (PubMed:14722618, PubMed:21593315). Contributes to Ca(2+) transport during excitation-contraction coupling in muscle (PubMed:14722618). In neurons, contributes to the rapid decrease of cytoplasmic Ca(2+) levels back to baseline after neuronal activation, and thereby contributes to modulate synaptic plasticity, learning and memory (PubMed:21593315). Required for normal oligodendrocyte differentiation and for normal myelination (PubMed:21959935). Mediates Ca(2+) efflux from mitochondria and contributes to mitochondrial Ca(2+) ion homeostasis (PubMed:24616101). Isoform 1 displays higher calcium exchanger activity than isoform 2, probably because isoform 1 has a lower threshold for activation by cytoplasmic Ca(2+) (PubMed:24616101).UniProtKB/Swiss-Prot Function
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"NM_080440" in other vectors (4)
SKU Description Size Price
MC222653 Slc8a3 (untagged) - Mouse solute carrier family 8 (sodium/calcium exchanger), member 3 (Slc8a3), transcript variant 2, (10ug) 10 ug
$936.00
MG221680 Slc8a3 (tGFP-tagged) - Mouse solute carrier family 8 (sodium/calcium exchanger) member 3 (Slc8a3) transcript variant 2, (10ug) 10 ug
$1,050.00
MR221680 Slc8a3 (Myc-DDK-tagged) - Mouse solute carrier family 8 (sodium/calcium exchanger), member 3 (Slc8a3), transcript variant 2 10 ug
$589.00 $850.00
MR221680L3 Lenti ORF clone of Slc8a3 (Myc-DDK-tagged) - Mouse solute carrier family 8 (sodium/calcium exchanger), member 3 (Slc8a3), transcript variant 2 10 ug
$1,150.00

Plasmid Map for MR221680L4

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file-download TXT (FASTA format)

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Cloning scheme for MR221680L4
Circular map for MR221680L4

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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