Col8a2 (NM_199473) Mouse Tagged Lenti ORF Clone

  • LentiORF®
SKU
MR210118L4
Lenti ORF clone of Col8a2 (mGFP-tagged) - Mouse collagen, type VIII, alpha 2 (Col8a2)
  $941.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Col8a2
Synonyms AI429819
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(MR210118).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_199473
ORF Size 2097 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_199473.2
RefSeq Size 4331 bp
RefSeq ORF 2100 bp
Locus ID 329941
UniProt ID P25318
Cytogenetics 4 60.29 cM
Summary Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in atherogenesis (By similarity).UniProtKB/Swiss-Prot Function
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Resources
"NM_199473" in other vectors (4)
SKU Description Size Price
MC220630 Col8a2 (untagged) - Mouse collagen, type VIII, alpha 2 (Col8a2), (10ug) 10 ug
$705.00
MG210118 Col8a2 (tGFP-tagged) - Mouse procollagen, type VIII, alpha 2 (Col8a2) 10 ug
$841.00
MR210118 Col8a2 (Myc-DDK-tagged) - Mouse collagen, type VIII, alpha 2 (Col8a2) 10 ug
$589.00 $641.00
MR210118L3 Lenti ORF clone of Col8a2 (Myc-DDK-tagged) - Mouse collagen, type VIII, alpha 2 (Col8a2) 10 ug
$941.00

Plasmid Map for MR210118L4

Download sequence for

Cloning scheme for MR210118L4

Circular map for MR210118L4

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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