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Ly9 (NM_008534) Mouse Tagged Lenti ORF Clone

  • LentiORF®
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SKU
MR209585L4
Lenti ORF clone of Ly9 (mGFP-tagged) - Mouse lymphocyte antigen 9 (Ly9)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
mGFP tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $909.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ly9
Synonyms AI893573; CD229; Lgp100; Ly-9; SLAMF3; T100
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(MR209585).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008534
ORF Size 1962 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008534.3, NP_032560.2
RefSeq Size 2449 bp
RefSeq ORF 1965 bp
Locus ID 17085
UniProt ID Q01965
Cytogenetics 1 79.54 cM
Summary Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2 (PubMed:19648922). May participate in adhesion reactions between T lymphocytes and accessory cells by homophilic interaction. Promotes T-cell differentiation into a helper T-cell Th17 phenotype leading to increased IL-17 secretion; the costimulatory activity requires SH2D1A. Promotes recruitment of RORC to the IL-17 promoter (By similarity). May be involved in the maintenance of peripheral cell tolerance by serving as a negative regulator of the immune response. May disable autoantibody responses and inhibit IFN-gamma secretion by CD4(+) T-cells (PubMed:23914190). May negatively regulate the size of thymic innate CD8(+) T-cells and the development of invariant natural killer T (iNKT) cells (PubMed:23225888). Can promote natural killer (NK) cell activation (PubMed:19648922).UniProtKB/Swiss-Prot Function
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"NM_008534" in other vectors (4)
SKU Description Size Price
MC220159 Ly9 (untagged) - Mouse lymphocyte antigen 9 (Ly9), (10ug) 10 ug
$671.00
MG209585 Ly9 (tGFP-tagged) - Mouse lymphocyte antigen 9 (Ly9) 10 ug
$809.00
MR209585 Ly9 (Myc-DDK-tagged) - Mouse lymphocyte antigen 9 (Ly9) 10 ug
$289.00 $609.00
MR209585L3 Lenti ORF clone of Ly9 (Myc-DDK-tagged) - Mouse lymphocyte antigen 9 (Ly9) 10 ug
$909.00

Plasmid Map for MR209585L4

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MR209585L4
Circular map for MR209585L4

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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