Adpgk (NM_028121) Mouse Tagged Lenti ORF Clone

  • LentiORF®
SKU
MR207965L4
Lenti ORF clone of Adpgk (mGFP-tagged) - Mouse ADP-dependent glucokinase (Adpgk)
  $757.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Adpgk
Synonyms 2610017G09Rik; Adp-gk; C77577
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(MR207965).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028121
ORF Size 1488 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028121.2, NP_082397.1
RefSeq Size 2499 bp
RefSeq ORF 1491 bp
Locus ID 72141
UniProt ID Q8VDL4
Cytogenetics 9 B
Summary Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_028121" in other vectors (4)
SKU Description Size Price
MC204303 Adpgk (untagged) - Mouse ADP-dependent glucokinase (Adpgk), (10ug) 10 ug
$457.00
MG207965 Adpgk (tGFP-tagged) - Mouse ADP-dependent glucokinase (Adpgk) 10 ug
$657.00
MR207965 Adpgk (Myc-DDK-tagged) - Mouse ADP-dependent glucokinase (Adpgk) 10 ug
$289.00 $457.00
MR207965L3 Lenti ORF clone of Adpgk (Myc-DDK-tagged) - Mouse ADP-dependent glucokinase (Adpgk) 10 ug
$757.00

Plasmid Map for MR207965L4

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Cloning scheme for MR207965L4

Circular map for MR207965L4

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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