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Pabpn1 (NM_019402) Mouse Tagged Lenti ORF Clone

  • LentiORF®
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SKU
MR204225L4
Lenti ORF clone of Pabpn1 (mGFP-tagged) - Mouse poly(A) binding protein, nuclear 1 (Pabpn1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
mGFP tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $600.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pabpn1
Synonyms mPABII; PAB2; Pabp3
Vector pLenti-C-mGFP-P2A-Puro
E. coli Selection Chloramphenicol (34 ug/mL)
Mammalian Cell Selection Puromycin
Sequence Data
ORF Nucleotide Sequence
The ORF insert of this clone is exactly the same as(MR204225).
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019402
ORF Size 906 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019402.2, NP_062275.1
RefSeq Size 1793 bp
RefSeq ORF 909 bp
Locus ID 54196
UniProt ID Q8CCS6
Cytogenetics 14 27.98 cM
Summary Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length. Increases the affinity of poly(A) polymerase for RNA. Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes. Binds to poly(A) and to poly(G) with high affinity. May protect the poly(A) tail from degradation. Subunit of the trimeric poly(A) tail exosome targeting (PAXT) complex, a complex that directs a subset of long and polyadenylated poly(A) RNAs for exosomal degradation. The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its cofactor MTREX, which links to RNA-binding protein adapters (By similarity).UniProtKB/Swiss-Prot Function
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"NM_019402" in other vectors (4)
SKU Description Size Price
MC205561 Pabpn1 (untagged) - Mouse poly(A) binding protein, nuclear 1 (Pabpn1), (10ug) 10 ug
$300.00
MG204225 Pabpn1 (tGFP-tagged) - Mouse poly(A) binding protein, nuclear 1 (Pabpn1) 10 ug
$500.00
MR204225 Pabpn1 (Myc-DDK-tagged) - Mouse poly(A) binding protein, nuclear 1 (Pabpn1) 10 ug
$289.00 $300.00
MR204225L3 Lenti ORF clone of Pabpn1 (Myc-DDK-tagged) - Mouse poly(A) binding protein, nuclear 1 (Pabpn1) 10 ug
$600.00

Plasmid Map for MR204225L4

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Cloning scheme for MR204225L4
Circular map for MR204225L4

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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