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Cdkn2a (NM_001040654) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG227284
Cdkn2a (tGFP-tagged) - Mouse cyclin-dependent kinase inhibitor 2A (Cdkn2a) transcript variant 2, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
  $650.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cdkn2a
Synonyms Arf; ARF-INK4a; INK4a-ARF; Ink4a/Arf; MTS1; p16; p16(INK4a); p16INK4a; p19; p19ARF; Pctr1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG227284 representing NM_001040654
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGTCCGCTGCAGACAGACTGGCCAGGGCGGCGGCCCAGGGCCGTGTGCATGACGTGCGGGCACTGC
TGGAAGCCGGGGTTTCGCCCAACGCCCCGAACTCTTTCGGTCGTACCCCGATTCAGGTGATGATGATGGG
CAACGTTCACGTAGCAGCTCTTCTGCTCAACTACGGTGCAGATTCGAACTGCGAGGACCCCACTACCTTC
TCCCGCCCGGTGCACGACGCAGCGCGGGAAGGCTTCCTGGACACGCTGGTGGTGCTGCACGGGTCAGGGG
CTCGGCTGGATGTGCGCGATGCCTGGGGTCGCCTGCCGCTCGACTTGGCCCAAGAGCGGGGACATCAAGA
CATCGTGCGATATTTGCGTTCCGCTGGGTGCTCTTTGTGTTCCGCTGGGTGGTCTTTGTGTACCGCTGGG
AACGTCGCCCAGACCGACGGGCATAGCTTCAGCTCAAGCACGCCCAGGGCCCTGGAACTTCGCGGCCAAT
CCCAAGAGCAGAGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG227284 representing NM_001040654
Red=Cloning site Green=Tags(s)

MESAADRLARAAAQGRVHDVRALLEAGVSPNAPNSFGRTPIQVMMMGNVHVAALLLNYGADSNCEDPTTF
SRPVHDAAREGFLDTLVVLHGSGARLDVRDAWGRLPLDLAQERGHQDIVRYLRSAGCSLCSAGWSLCTAG
NVAQTDGHSFSSSTPRALELRGQSQEQS

TRTRPLE - GFP Tag - V
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001040654
ORF Size 504 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001040654.1, NP_001035744.1
RefSeq Size 850 bp
RefSeq ORF 507 bp
Locus ID 12578
UniProt ID P51480
Cytogenetics 4 42.15 cM
Summary Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.UniProtKB/Swiss-Prot Function
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"NM_001040654" in other vectors (5)
SKU Description Size Price
MR227284 Cdkn2a (Myc-DDK-tagged) - Mouse cyclin-dependent kinase inhibitor 2A (Cdkn2a), transcript variant 2 10 ug
$450.00
MR227284L1 Lenti ORF clone of Cdkn2a (Myc-DDK-tagged) - Mouse cyclin-dependent kinase inhibitor 2A (Cdkn2a), transcript variant 2 10 ug
$750.00
MR227284L2 Lenti ORF clone of Cdkn2a (mGFP-tagged) - Mouse cyclin-dependent kinase inhibitor 2A (Cdkn2a), transcript variant 2 10 ug
$750.00
MR227284L3 Lenti ORF clone of Cdkn2a (Myc-DDK-tagged) - Mouse cyclin-dependent kinase inhibitor 2A (Cdkn2a), transcript variant 2 10 ug
$750.00
MR227284L4 Lenti ORF clone of Cdkn2a (mGFP-tagged) - Mouse cyclin-dependent kinase inhibitor 2A (Cdkn2a), transcript variant 2 10 ug
$750.00

Plasmid Map for MG227284

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG227284
Circular map for MG227284

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