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Tgfbr2 (NM_029575) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG226791
Tgfbr2 (tGFP-tagged) - Mouse transforming growth factor beta receptor II (Tgfbr2) transcript variant 2, (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
$489.00 $781.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tgfbr2
Synonyms 1110020H15Rik; AU042018; DNIIR; RIIDN; TbetaR-II; TbetaRII; TBR-II
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG226791 representing NM_029575
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTCGGGGGCTGCTCCGGGGCCTGTGGCCGCTGCATATCGTCCTGTGGACGCGCATCGCCAGCACGA
TCCCGCCGCACGTTCCCAAGTCGGTTAACAGTGATGTCATGGCCAGCGACAATGGCGGTGCGGTCAAGCT
TCCACAGCTGTGCAAGTTTTGCGATGTGAGACTGTCCACTTGCGACAACCAGAAGTCCTGCATGAGCAAC
TGCAGCATCACGGCCATCTGTGAGAAGCCGCATGAAGTCTGCGTGGCCGTGTGGAGGAAGAACGACAAGA
ACATTACTCTGGAGACGGTTTGCCACGACCCCAAGCTCACCTACCACGGCTTCACTCTGGAAGATGCCGC
TTCTCCCAAGTGTGTCATGAAGGAAAAGAAAAGGGCGGGCGAGACTTTCTTCATGTGTGCCTGTAACATG
GAAGAGTGCAACGATTACATCATCTTTTCGGAAGAATACACCACCAGCAGTCCCGACCTGTTGTTGGTCA
TTATCCAAGTGACGGGTGTCAGCCTCCTGCCTCCGCTGGGGATTGCCATAGCTGTCATCATCATCTTCTA
CTGCTACCGTGTCCACCGGCAGCAGAAGCTGAGCCCGTCCTGGGAGAGCAGCAAGCCCCGGAAACTGATG
GATTTCAGTGACAATTGTGCCATCATCCTGGAGGACGACCGCTCCGACATCAGCTCCACGTGCGCCAACA
ACATCAACCACAACACGGAGCTGCTGCCCATCGAGCTGGACACGCTGGTGGGGAAGGGCCGCTTCGCCGA
GGTCTACAAGGCCAAGCTGAAGCAGAACACCTCAGAGCAGTTTGAGACCGTGGCTGTCAAGATCTTCCCC
TACGAGGAGTACTCCTCGTGGAAAACAGAGAAGGACATCTTCTCCGATATCAACCTGAAGCATGAGAACA
TCCTGCAGTTCCTGACGGCCGAGGAGCGGAAGACAGAGCTGGGCAAGCAGTACTGGCTGATCACGGCGTT
CCACGCGAAGGGCAACCTGCAGGAGTACCTCACGAGGCATGTCATCAGCTGGGAGGACCTGAGGAAGCTG
GGCAGCTCCCTGGCCCGGGGCATCGCTCATCTCCACAGTGACCACACTCCTTGTGGGAGGCCCAAGATGC
CCATTGTTCACAGGGACCTCAAGAGCTCTAACATCCTAGTGAAGAACGACTTGACCTGTTGCCTGTGTGA
CTTCGGGCTGTCCTTGCGCCTGGACCCTACTCTGTCTGTGGATGACCTGGCCAACAGCGGGCAGGTGGGA
ACGGCAAGATACATGGCCCCGGAAGTTCTAGAATCCAGGATGAATCTGGAAAACGTGGAGTCGTTCAAGC
AGACGGATGTCTACTCCATGGCTCTGGTACTCTGGGAAATGACGTCCCGCTGCAATGCTGTGGGAGAAGT
GAAGGATTACGAGCCCCCATTTGGTTCCAAGGTGCGGGAGCACCCCTGTGTGGAGAGCATGAAAGACAGT
GTGCTGAGAGACCGAGGGCGGCCGGAAATTCCCAGCTTCTGGCTCAACCACCAGGGCATCCAGATCGTGT
GTGAGACTTTGACCGAGTGCTGGGACCATGACCCCGAAGCCCGTCTCACAGCACAGTGTGTGGCAGAGCG
CTTCAGTGAGCTGGAGCATCCGGAGAGACTCTCTGGGAGGAGCTGCTCCCAGGAGAAGATTCCAGAAGAT
GGCTCGCTGAACACTACCAAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG226791 representing NM_029575
Red=Cloning site Green=Tags(s)

MGRGLLRGLWPLHIVLWTRIASTIPPHVPKSVNSDVMASDNGGAVKLPQLCKFCDVRLSTCDNQKSCMSN
CSITAICEKPHEVCVAVWRKNDKNITLETVCHDPKLTYHGFTLEDAASPKCVMKEKKRAGETFFMCACNM
EECNDYIIFSEEYTTSSPDLLLVIIQVTGVSLLPPLGIAIAVIIIFYCYRVHRQQKLSPSWESSKPRKLM
DFSDNCAIILEDDRSDISSTCANNINHNTELLPIELDTLVGKGRFAEVYKAKLKQNTSEQFETVAVKIFP
YEEYSSWKTEKDIFSDINLKHENILQFLTAEERKTELGKQYWLITAFHAKGNLQEYLTRHVISWEDLRKL
GSSLARGIAHLHSDHTPCGRPKMPIVHRDLKSSNILVKNDLTCCLCDFGLSLRLDPTLSVDDLANSGQVG
TARYMAPEVLESRMNLENVESFKQTDVYSMALVLWEMTSRCNAVGEVKDYEPPFGSKVREHPCVESMKDS
VLRDRGRPEIPSFWLNHQGIQIVCETLTECWDHDPEARLTAQCVAERFSELEHPERLSGRSCSQEKIPED
GSLNTTK

TRTRPLE - GFP Tag - V
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_029575
ORF Size 1701 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_029575.3, NP_083851.3
RefSeq Size 4728 bp
RefSeq ORF 1704 bp
Locus ID 21813
Cytogenetics 9 68.39 cM
Summary Transmembrane serine/threonine kinase forming with the TGF-beta type I serine/threonine kinase receptor, TGFBR1, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFRB1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways (By similarity).UniProtKB/Swiss-Prot Function
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"NM_029575" in other vectors (4)
SKU Description Size Price
MC219060 Tgfbr2 (untagged) - Mouse transforming growth factor, beta receptor II (Tgfbr2), transcript variant 2, (10ug) 10 ug
$529.00
MR226791 Tgfbr2 (Myc-DDK-tagged) - Mouse transforming growth factor, beta receptor II (Tgfbr2), transcript variant 2 10 ug
$581.00
MR226791L3 Lenti ORF clone of Tgfbr2 (Myc-DDK-tagged) - Mouse transforming growth factor, beta receptor II (Tgfbr2), transcript variant 2 10 ug
$881.00
MR226791L4 Lenti ORF clone of Tgfbr2 (mGFP-tagged) - Mouse transforming growth factor, beta receptor II (Tgfbr2), transcript variant 2 10 ug
$881.00

Plasmid Map for MG226791

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG226791
Circular map for MG226791

file-alt Citations

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