Terf2 (NM_009353) Mouse Tagged ORF Clone
- TrueORF®
SKU
MG226635
Terf2 (tGFP-tagged) - Mouse telomeric repeat binding factor 2 (Terf2) transcript variant 1, (10ug)
$1,004.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Terf2 |
| Synonyms | TRF2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_009353 |
| ORF Size | 1620 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_009353.2, NP_033379.2 |
| RefSeq Size | 2664 bp |
| RefSeq ORF | 1623 bp |
| Locus ID | 21750 |
| Cytogenetics | 8 53.59 cM |
| Summary | Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_009353" in other vectors (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MR226635 | Terf2 (Myc-DDK-tagged) - Mouse telomeric repeat binding factor 2 (Terf2), transcript variant 1 | 10 ug |
$804.00
|
|
| MR226635L3 | Lenti ORF clone of Terf2 (Myc-DDK-tagged) - Mouse telomeric repeat binding factor 2 (Terf2), transcript variant 1 | 10 ug |
$1,104.00
|
|
| MR226635L4 | Lenti ORF clone of Terf2 (mGFP-tagged) - Mouse telomeric repeat binding factor 2 (Terf2), transcript variant 1 | 10 ug |
$1,104.00
|
|
Citations
Transcription regulation of CDKN1A (p21/CIP1/WAF1) by TRF2 is epigenetically controlled through the REST repressor complex
Hussain, Saha, Purohit et al
Sci Rep (2017) 7 (1), 11541 DOI: 10.1038/s41598-017-11177-1
Hussain, Saha, Purohit et al
Sci Rep (2017) 7 (1), 11541 DOI: 10.1038/s41598-017-11177-1
AI Summary: OriGene products contributed to this study by providing the REST antibody (OriGene#TA330562) for protein detection and the pCMV6-TRF2 plasmid for TRF2 overexpression experiments, enabling investigation of the molecular mechanisms underlying TRF2-mediated repression of p21.
Snippet: … REST (Origene#TA330562), co-REST (Abcam#32631), anti-Rap1 (sc-28197), anti-H3K4Me (Abcam 106165), anti-H3K4Me2 (Abcam 32356). … pCMV6- TRF2 plasmid was purchased from Origene. … REST (Origene#TA330562), co-REST (Abcam#32631), anti-Rap1 (sc-28197), anti-H3K4Me (Abcam 106165), anti-H3K4Me2 (Abcam 32356). … pCMV6- TRF2 plasmid was purchased from Origene. … REST (Origene#TA330562), co-REST (Abcam#32631), anti-Rap1 (sc-28197), anti-H3K4Me (Abcam 106165), anti-H3K4Me2 (Abcam 32356). … pCMV6- TRF2 plasmid was purchased from Origene. …
Variant repeats are interspersed throughout the telomeres and recruit nuclear receptors in ALT cells
Conomos, Stutz, Hills et al
J. Cell Biol. (2012) 199 (6), 893-906 DOI: 10.1083/jcb.201207189
Conomos, Stutz, Hills et al
J. Cell Biol. (2012) 199 (6), 893-906 DOI: 10.1083/jcb.201207189
Cited by 170
AI Summary: OriGene products contributed to this study by providing the pCMV6-TR4 (RC211232) and pCMV6-COUP-TF2 (RC210598) expression plasmids, which were used to investigate the recruitment of nuclear receptors to telomeres in ALT cells.
Snippet: dependent on telomere length. Variant repeats present in the proximal regions of the telomeres will therefore be overrepresented in mortal and telomerase-positive cell lines with short telomeres. Consequently, the abundance
Showing 1-2 of 2 papers.
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