Cd9 (NM_007657) Mouse Tagged ORF Clone
$650.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Cd9 |
| Synonyms | Tspan29 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_007657 |
| ORF Size | 678 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_007657.4 |
| RefSeq Size | 1306 bp |
| RefSeq ORF | 681 bp |
| Locus ID | 12527 |
| UniProt ID | P40240 |
| Cytogenetics | 6 59.32 cM |
| Summary | Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion (PubMed:10700183, PubMed:10634790, PubMed:10634791, PubMed:14715942). Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion (PubMed:10700183, PubMed:10634790, PubMed:10634791, PubMed:21690351). In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration (PubMed:23575678). In macrophages, associates with CD9 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles. Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption (PubMed:12796480). Acts as a receptor for PSG17 (PubMed:11805154). Involved in platelet activation and aggregation (PubMed:14715942). Regulates paranodal junction formation (PubMed:14715942). Involved in cell adhesion, cell motility and tumor metastasis (By similarity). Also regulates integrin-dependent migration of macrophages, particularly relevant for inflammatory response in the lung (PubMed:18662991).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_007657" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC208269 | Cd9 (untagged) - Mouse CD9 antigen (Cd9), (10ug) | 10 ug |
$480.00
|
|
| MR226288 | Cd9 (Myc-DDK-tagged) - Mouse CD9 antigen (Cd9) | 10 ug |
$450.00
|
|
| MR226288L3 | Lenti ORF clone of Cd9 (Myc-DDK-tagged) - Mouse CD9 antigen (Cd9) | 10 ug |
$750.00
|
|
| MR226288L4 | Lenti ORF clone of Cd9 (mGFP-tagged) - Mouse CD9 antigen (Cd9) | 10 ug |
$750.00
|
|
Citations
Juno and CD9 protein network organization in oolemma of mouse oocyte
Frolikova, Sur, Novotny et al
Front Cell Dev Biol (2023) 11, 1110681 DOI: 10.3389/fcell.2023.1110681
Frolikova, Sur, Novotny et al
Front Cell Dev Biol (2023) 11, 1110681 DOI: 10.3389/fcell.2023.1110681
Cited by 6
AI Summary: OriGene products, specifically the mouse CD9-GFP plasmid DNA (MG226288) and mouse Juno plasmid DNA (MC207552), were used to transfect HEK293T/17 cells, enabling the study of the interaction and colocalization of Juno and CD9 proteins in the mouse oolemma.
Snippet: …When cells reached 70–80% of confluence, they were co-transfected by 3 µg of mouse CD9-GFP plasmid DNA (MG226288, Origene, MD, USA) and 3 µg of mouse Juno plasmid DNA (MC207552, Origene) and left for 16 h in DMEM cultivation medium with 10% of FBS without antibiotics. …
Sperm-oocyte signaling: the role of IZUMO1R and CD9 in PTK2B activation and actin remodeling at the sperm binding site
Wang, Hong, Kinsey
Biol Reprod (2021)
Wang, Hong, Kinsey
Biol Reprod (2021)
Cited by 16
AI Summary: OriGene’s mouse cd9 clone (#MG226288) was used to express murine CD9 in cd9-null oocytes, enabling the authors to investigate CD9’s role in PTK2B activation and actin remodeling during sperm-oocyte signaling.
Snippet: Expression of murine cd9 in oocytes null for mouse cd9 The mouse cd9 clone (# MG226288, ORIGENE (Rockville, MD)) was used to transfect E. coli (CopyCutter C400CH10, Lucigen Corporation (Middleton, WI)) and the sequence of the insert was confirmed;- Expression of murine cd9 in oocytes null for mouse cd9. The mouse cd9 clone (# MG226288, ORIGENE (Rockville, MD)) was used to transfect E. coli (CopyCutter C400CH10, Lucigen Corporation (Middleton, WI)) and the sequence of the insert was confirmed
A role for tetraspanin proteins in regulating fusion induced by Burkholderia thailandensis
Elgawidi, Mohsin, Ali et al
Med. Microbiol. Immunol. (2020)
Elgawidi, Mohsin, Ali et al
Med. Microbiol. Immunol. (2020)
Cited by 13
AI Summary: OriGene products pCMV6-AV-mCD9-GFP and pCMV-AV-GFP were used to generate J774.2 macrophage cell lines overexpressing CD9-GFP or GFP, enabling the study of CD9's role in Burkholderia thailandensis-induced cell fusion.
Snippet: J774.2 stably overexpressing mouse CD9- GFP and GFP were generated by transfection with pCMV6- AV-mCD9-GFP or pCMV-AV-GFP (Origene) using elec- troporation followed by selection in G418-containing medium and further selection by fluorescence activated;- J774.2 stably overexpressing mouse CD9- GFP and GFP were generated by transfection with pCMV6- AV-mCD9-GFP or pCMV-AV-GFP (Origene) using elec- troporation followed by selection in G418-containing medium and further selection by fluorescence activated
Showing 1-3 of 3 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
