Tgfb2 (NM_009367) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG225633
Tgfb2 (tGFP-tagged) - Mouse transforming growth factor beta 2 (Tgfb2), (10ug)
  $886.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tgfb2
Synonyms BB105277; Tgf-beta; Tgf-beta2; Tgfb-2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG225633 representing NM_009367
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCACTACTGTGTGCTGAGCACCTTTTTGCTCCTGCATCTGGTCCCGGTGGCGCTCAGTCTGTCTACCT
GCAGCACCCTCGACATGGATCAGTTTATGCGCAAGAGGATCGAGGCCATCCGCGGGCAGATCCTGAGCAA
GCTGAAGCTCACCAGCCCCCCGGAAGACTATCCGGAGCCGGATGAGGTCCCCCCGGAGGTGATTTCCATC
TACAACAGTACCAGGGACTTACTGCAGGAGAAGGCAAGCCGGAGGGCAGCCGCCTGCGAGCGCGAGCGGA
GCGACGAGGAGTACTACGCCAAGGAGGTTTATAAAATCGACATGCCGTCCCACCTCCCCTCCGAAAATGC
CATCCCGCCCACTTTCTACAGACCCTACTTCAGAATCGTCCGCTTTGATGTCTCAACAATGGAGAAAAAT
GCTTCGAATCTGGTGAAGGCAGAGTTCAGGGTCTTCCGCTTGCAAAACCCCAAAGCCAGAGTGGCCGAGC
AGCGGATTGAACTGTATCAGATCCTTAAATCCAAAGACTTAACATCTCCCACCCAGCGCTACATCGATAG
CAAGGTTGTGAAAACCAGAGCGGAGGGTGAATGGCTCTCCTTCGACGTGACAGACGCTGTGCAGGAGTGG
CTTCACCACAAAGACAGGAACCTGGGGTTTAAAATAAGTTTACACTGCCCCTGCTGTACCTTCGTGCCGT
CTAATAATTACATCATCCCGAATAAAAGCGAAGAGCTCGAGGCGAGATTTGCAGGTATTGATGGCACCTC
TACATATGCCAGTGGTGATCAGAAAACTATAAAGTCCACTAGGAAAAAAACCAGTGGGAAGACCCCACAT
CTCCTGCTAATGTTGTTGCCCTCCTACAGACTGGAGTCACAACAGTCCAGCCGGCGGAAGAAGCGCGCTT
TGGATGCTGCCTACTGCTTTAGAAATGTGCAGGATAATTGCTGCCTTCGCCCTCTTTACATTGATTTTAA
GAGGGATCTTGGATGGAAATGGATCCATGAACCCAAAGGGTACAATGCTAACTTCTGTGCTGGGGCATGC
CCATATCTATGGAGTTCAGACACTCAACACACCAAAGTCCTCAGCCTGTACAACACCATAAATCCCGAAG
CTTCCGCTTCCCCTTGCTGTGTGTCCCAGGATCTGGAACCACTGACCATTCTCTATTACATTGGAAATAC
GCCCAAGATCGAACAGCTTTCCAATATGATTGTCAAGTCTTGTAAATGCAGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG225633 representing NM_009367
Red=Cloning site Green=Tags(s)

MHYCVLSTFLLLHLVPVALSLSTCSTLDMDQFMRKRIEAIRGQILSKLKLTSPPEDYPEPDEVPPEVISI
YNSTRDLLQEKASRRAAACERERSDEEYYAKEVYKIDMPSHLPSENAIPPTFYRPYFRIVRFDVSTMEKN
ASNLVKAEFRVFRLQNPKARVAEQRIELYQILKSKDLTSPTQRYIDSKVVKTRAEGEWLSFDVTDAVQEW
LHHKDRNLGFKISLHCPCCTFVPSNNYIIPNKSEELEARFAGIDGTSTYASGDQKTIKSTRKKTSGKTPH
LLLMLLPSYRLESQQSSRRKKRALDAAYCFRNVQDNCCLRPLYIDFKRDLGWKWIHEPKGYNANFCAGAC
PYLWSSDTQHTKVLSLYNTINPEASASPCCVSQDLEPLTILYYIGNTPKIEQLSNMIVKSCKCS

TRTRPLE - GFP Tag - V
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009367
ORF Size 1242 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009367.4
RefSeq Size 4725 bp
RefSeq ORF 1245 bp
Locus ID 21808
UniProt ID P27090
Cytogenetics 1 89.95 cM
Summary This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGF-beta family members. Mice lacking a functional copy of this gene display developmental defects in multiple organs and perinatal lethality. Heterozygous mutant mice exhibit aortic root aneurysm. This gene encodes multiple isoforms that may undergo similar proteolytic processing. provided by RefSeq, Aug 2016
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_009367" in other vectors (4)
SKU Description Size Price
MC200993 Tgfb2 (untagged) - Mouse transforming growth factor, beta 2 (Tgfb2), (10ug) 10 ug
$686.00
MR225633 Tgfb2 (Myc-DDK-tagged) - Mouse transforming growth factor, beta 2 (Tgfb2) 10 ug
$686.00
MR225633L3 Lenti ORF clone of Tgfb2 (Myc-DDK-tagged) - Mouse transforming growth factor, beta 2 (Tgfb2) 10 ug
$986.00
MR225633L4 Lenti ORF clone of Tgfb2 (mGFP-tagged) - Mouse transforming growth factor, beta 2 (Tgfb2) 10 ug
$986.00

Plasmid Map for MG225633

Download sequence for

Cloning scheme for MG225633

Circular map for MG225633

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products