Cdca8 (NM_026560) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG223484
Cdca8 (tGFP-tagged) - Mouse cell division cycle associated 8 (Cdca8), (10ug)
  $650.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cdca8
Synonyms 4831429J16Rik; AU044747; BOR; Borealin; D4Ertd421e; DasraB; MESRGP
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG223484 representing NM_026560
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCCCAAGAAACGCAGCAGCCGCGGAACCAGGACCAACACGCTGCGGAGCCGGAAGCTCGCCTCCT
TCCTGAAGGACTTCGACCGCGAGGTGCAAGTTCGAACCAAGCAAATTGAGTCCGACAGACAGACCCTCCT
CAAGGAGGTGGAAAATCTGTACAACATCGAGATCCTTCGGCTCCCCAAGGCGCTGCAAGGGATGAAGTGG
CTTGACTACTTCGCCCTAGGAGGAAACAAGCAGGCCCTGGAAGAGGCAGCAAAAGCTGATCGAGACATCA
CAGAAATAAACAATTTAACAGCTGAAGCTATTCAGACACCTTTGAAATCTGTTAAAAAGCGAAAGGTAAT
CGAGGTGGAGGAATCGATAAAGGAAGAAGAAGAAGAGGAAGAAGAAGGAGGAGGAGGAGGAGGAAGAACA
AAAAAGAGCCATAAGAATCTTCGATCTGCAAAAGTCAAAAGATGCCTTCCATCCAAGAAGAGAACCCAGT
CCATACAAGGAAGAGGCAGAAGTAAAAGGTTAAGCCATGACTTTGTGACGCCAGCTATGAGCAGGCTGGA
GCCGTCTCTGGTGAAACCAACCCCAGGCATGACACCTAGGTTTGACTCCCGGGTCTTCAAGACTCCAGGG
CTACGCACTCCAGCAGCCAAAGAGCAAGTTTACAACATCTCCATCAACGGCAGCCCTCTCGCAGACAGCA
AAGAGATCTCCCTCAGTGTGCCCATAGGTGGCGGTGCGAGCTTGCGGTTATTGGCCAGTGACTTGCAAAG
GATTGATATTGCTCAGCTGAATCCAGAGGCTCTGGGAAACATTAGAAAGCTCTCGAGCCGCCTCGCCCAG
ATCTGCAGCAGCATACGGACGGGCCGA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG223484 representing NM_026560
Red=Cloning site Green=Tags(s)

MAPKKRSSRGTRTNTLRSRKLASFLKDFDREVQVRTKQIESDRQTLLKEVENLYNIEILRLPKALQGMKW
LDYFALGGNKQALEEAAKADRDITEINNLTAEAIQTPLKSVKKRKVIEVEESIKEEEEEEEEGGGGGGRT
KKSHKNLRSAKVKRCLPSKKRTQSIQGRGRSKRLSHDFVTPAMSRLEPSLVKPTPGMTPRFDSRVFKTPG
LRTPAAKEQVYNISINGSPLADSKEISLSVPIGGGASLRLLASDLQRIDIAQLNPEALGNIRKLSSRLAQ
ICSSIRTGR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026560
ORF Size 867 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026560.4, NP_080836.3
RefSeq Size 1626 bp
RefSeq ORF 870 bp
Locus ID 52276
UniProt ID Q8BHX3
Cytogenetics 4 57.93 cM
Summary Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment (By similarity).UniProtKB/Swiss-Prot Function
Reviews
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_026560" in other vectors (6)
SKU Description Size Price
MC209893 Cdca8 (untagged) - Mouse cell division cycle associated 8 (Cdca8), (10ug) 10 ug
$450.00
MR223484 Cdca8 (Myc-DDK-tagged) - Mouse cell division cycle associated 8 (Cdca8) 10 ug
$450.00
MR223484L1 Lenti ORF clone of Cdca8 (Myc-DDK-tagged) - Mouse cell division cycle associated 8 (Cdca8) 10 ug
$750.00
MR223484L2 Lenti ORF clone of Cdca8 (mGFP-tagged) - Mouse cell division cycle associated 8 (Cdca8) 10 ug
$750.00
MR223484L3 Lenti ORF clone of Cdca8 (Myc-DDK-tagged) - Mouse cell division cycle associated 8 (Cdca8) 10 ug
$750.00
MR223484L4 Lenti ORF clone of Cdca8 (mGFP-tagged) - Mouse cell division cycle associated 8 (Cdca8) 10 ug
$750.00

Plasmid Map for MG223484

Download sequence for

Cloning scheme for MG223484

Circular map for MG223484

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products