Cckbr (NM_007627) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG222564
Cckbr (tGFP-tagged) - Mouse cholecystokinin B receptor (Cckbr), (10ug)
  $886.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cckbr
Synonyms CCK-BR; CCK2; CCK2-R; CCK2R; CCKR-; CCKR-2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG222564 representing NM_007627
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCTGCTCAAGCTGAACCGCAGCTTGCAGGGACCAGGACCCGGGTCAGGGTCTTCTTTGTGCCGCC
CGGGTGTCTCCCTCCTCAACAGCAGTAGCGCCGGCAACCTCAGCTGCGAGACCCCTCGTATCCGCGGAAC
CGGGACCAGAGAATTGGAGTTGACCATTCGAATCACCCTTTATGCGGTGATCTTTCTGATGAGTGTTGGC
GGAAACGTGCTCATCATTGTGGTCCTGGGACTGAGCCGACGCCTAAGAACGGTCACCAACGCCTTCCTAC
TCTCCCTGGCAGTCAGCGACCTCCTGCTGGCTGTCGCTTGCATGCCCTTCACACTCCTGCCCAACCTCAT
GGGCACATTCATCTTCGGCACAGTCATCTGCAAGGCCGTTTCCTACCTCATGGGGGTATCAGTGAGTGTG
TCCACTCTAAATCTCGCGGCCATCGCCCTGGAGCGATACAGCGCCATCTGCCGACCACTGCAAGCACGAG
TGTGGCAGACACGCTCCCACGCAGCTCGGGTGATCTTAGCCACGTGGCTGCTGTCTGGATTGCTTATGGT
CCCCTACCCTGTGTACACCGTGGTGCAGCCAGTGGGGCCTCGAATACTGCAGTGCATGCATCTCTGGCCC
AGTGAACGTGTCCAACAAATGTGGTCCGTGCTTCTGCTAATACTGCTGTTCTTCATCCCGGGCGTGGTTA
TGGCAGTGGCCTATGGACTCATCTCCCGCGAACTCTACCTAGGGCTCCGCTTTGATGGTGATAATGACAG
CGAGACCCAAAGCCGGGTCCGAAACCAAGGGGGTCTGCCGGGTGGGGCAGCACCAGGGCCTGTCCAACAG
AACGGGGGTTGCCGGCATGTGACCAGCCTGACTGGGGAAGACAGTGATGGCTGCTACGTGCAACTTCCAC
GTTCCCGACTGGAGATGACAACGCTGACCACGCCCACTACTGGGCCAGGCCCTGGCCCTCGGCCCAACCA
GGCCAAGCTGCTGGCTAAGAAGCGGGTGGTGCGAATGCTGCTGGTGATTGTTTTGCTTTTTTTCGTGTGT
TGGCTGCCAGTGTACAGCGCCAACACGTGGCGTGCCTTCGATGGCCCAGGCGCGCGCCGAGCACTCGCAG
GGGCCCCTATCTCTTTCATCCACTTGCTGAGCTACACCTCTGCTTGTGCCAACCCCCTGGTCTACTGTTT
CATGCACCGCCGCTTCCGCCAGGCCTGCCTGGACACATGTGCCCGCTGTTGCCCACGCCCTCCACGAGCT
CGCCCACGGCCTCTTCCGGATGAGGATCCTCCTACCCCCTCCATCGCTTCTCTGTCCAGGCTAAGCTATA
CCACCATCAGCACGCTGGGGCCTGGC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG222564 representing NM_007627
Red=Cloning site Green=Tags(s)

MDLLKLNRSLQGPGPGSGSSLCRPGVSLLNSSSAGNLSCETPRIRGTGTRELELTIRITLYAVIFLMSVG
GNVLIIVVLGLSRRLRTVTNAFLLSLAVSDLLLAVACMPFTLLPNLMGTFIFGTVICKAVSYLMGVSVSV
STLNLAAIALERYSAICRPLQARVWQTRSHAARVILATWLLSGLLMVPYPVYTVVQPVGPRILQCMHLWP
SERVQQMWSVLLLILLFFIPGVVMAVAYGLISRELYLGLRFDGDNDSETQSRVRNQGGLPGGAAPGPVQQ
NGGCRHVTSLTGEDSDGCYVQLPRSRLEMTTLTTPTTGPGPGPRPNQAKLLAKKRVVRMLLVIVLLFFVC
WLPVYSANTWRAFDGPGARRALAGAPISFIHLLSYTSACANPLVYCFMHRRFRQACLDTCARCCPRPPRA
RPRPLPDEDPPTPSIASLSRLSYTTISTLGPG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007627
ORF Size 1356 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007627.4, NP_031653.1
RefSeq Size 2483 bp
RefSeq ORF 1362 bp
Locus ID 12426
UniProt ID P56481
Cytogenetics 7 55.86 cM
Summary This gene encodes a multipass transmembrane receptor protein expressed in the central nervous system and gastrointestinal tract. Cholecystokinin and gastrin bind to the encoded protein to stimulate gastric acid secretion and mucosal growth in the gastrointestinal tract, and anxiety, pain sensation and memory in the brain. Mice lacking the encoded protein exhibit an increase in the basal gastric pH and gastrin levels in the bloodstream as well as mild hypocalcemia, secondary hyperparathyroidism and increased bone resorption. provided by RefSeq, Apr 2015
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Documents
Resources
"NM_007627" in other vectors (6)
SKU Description Size Price
MC215922 Cckbr (untagged) - Mouse cholecystokinin B receptor (Cckbr), (10ug) 10 ug
$457.00
MR222564 Cckbr (Myc-DDK-tagged) - Mouse cholecystokinin B receptor (Cckbr) 10 ug
$686.00
MR222564L1 Lenti ORF clone of Cckbr (Myc-DDK-tagged) - Mouse cholecystokinin B receptor (Cckbr) 10 ug
$986.00
MR222564L2 Lenti ORF clone of Cckbr (mGFP-tagged) - Mouse cholecystokinin B receptor (Cckbr) 10 ug
$986.00
MR222564L3 Lenti ORF clone of Cckbr (Myc-DDK-tagged) - Mouse cholecystokinin B receptor (Cckbr) 10 ug
$986.00
MR222564L4 Lenti ORF clone of Cckbr (mGFP-tagged) - Mouse cholecystokinin B receptor (Cckbr) 10 ug
$986.00

Plasmid Map for MG222564

Download sequence for

Cloning scheme for MG222564

Circular map for MG222564

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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