Flot2 (NM_001040403) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG222238
Flot2 (tGFP-tagged) - Mouse flotillin 2 (Flot2) transcript variant 1, (10ug)
  $657.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Flot2
Synonyms AI573412; Esa; reggie-2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG222238 representing NM_001040403
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCAACTGCCACACGGTGGGCCCCAACGAGGCGCTGGTGGTCTCAGGAGGCTGTTGTGGTTCTGACT
ACAAACAGTATGTGTTTGGAGGCTGGGCTTGGGCCTGGTGGTGTATCTCGGACACTCAGAGGATTTCCCT
AGAGATTATGACGTTGCAGCCCCGCTGTGAGGACGTAGAGACGGCCGAGGGGGTAGCTTTAACTGTGACG
GGTGTCGCCCAGGTGAAGATCATGACGGAGAAGGAGCTCCTGGCTGTAGCCTGTGAACAGTTCCTGGGCA
AGAACGTACAGGACATTAAGAACGTCGTGCTGCAGACCCTGGAGGGGCATCTACGCTCTATCCTAGGGAC
TCTGACTGTAGAACAGATTTATCAGGACCGAGACCAGTTTGCCAAGCTGGTGCGGGAAGTGGCGGCCCCC
GATGTTGGCCGCATGGGCATCGAGATCCTCAGCTTCACCATCAAGGATGTTTATGACAAAGTAGACTATC
TGAGCTCCCTGGGCAAGACACAGACTGCTGTGGTGCAGAGAGATGCAGACATTGGTGTGGCAGAGGCAGA
ACGGGACGCAGGCATCCGGGAAGCTGAATGCAAGAAGGAGATGCTAGATGTGAAGTTCATGGCAGACACC
AAGATCGCTGACTCTAAGAGAGCCTTTGAGCTTCAAAAGTCAGCCTTCAGCGAGGAAGTCAACATCAAGA
CAGCTGAGGCCCAGTTGGCCTATGAGCTGCAAGGGGCCCGCGAGCAACAGAAGATCCGGCAGGAAGAGAT
TGAGATTGAGGTCGTACAGCGCAAGAAGCAGATCGCCGTGGAGGCGCAGGAGATCCTCCGCACCGACAAG
GAGCTCATCGCCACCGTGCGCCGCCCTGCAGAGGCTGAGGCCCACCGCATCCAGCAGATCGCTGAGGGCG
AAAAGGTGAAACAAGTCCTCTTGGCACAAGCAGAAGCTGAGAAGATTCGCAAAATTGGAGAGGCAGAGGC
AGCAGTCATTGAGGCAATGGGCAAGGCCGAGGCTGAGCGGATGAAGCTTAAGGCTGAGGCCTACCAGAAG
TATGGGGATGCAGCCAAGATGGCCTTGGTGCTGGAGGCCCTGCCCCAGATTGCTGCCAAGATCTCCGCGC
CCCTGACTAAAGTCGATGAGATTGTGGTTCTCAGTGGGGACAACAGCAAGGTGACATCAGAAGTAAACCG
GCTGCTAGCAGAACTGCCAGCCTCTGTGCACGCCCTCACCGGTGTGGACCTTTCAAAGATACCCCTGATC
AAGAACGCCACTGGTGCACAGGTG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG222238 representing NM_001040403
Red=Cloning site Green=Tags(s)

MGNCHTVGPNEALVVSGGCCGSDYKQYVFGGWAWAWWCISDTQRISLEIMTLQPRCEDVETAEGVALTVT
GVAQVKIMTEKELLAVACEQFLGKNVQDIKNVVLQTLEGHLRSILGTLTVEQIYQDRDQFAKLVREVAAP
DVGRMGIEILSFTIKDVYDKVDYLSSLGKTQTAVVQRDADIGVAEAERDAGIREAECKKEMLDVKFMADT
KIADSKRAFELQKSAFSEEVNIKTAEAQLAYELQGAREQQKIRQEEIEIEVVQRKKQIAVEAQEILRTDK
ELIATVRRPAEAEAHRIQQIAEGEKVKQVLLAQAEAEKIRKIGEAEAAVIEAMGKAEAERMKLKAEAYQK
YGDAAKMALVLEALPQIAAKISAPLTKVDEIVVLSGDNSKVTSEVNRLLAELPASVHALTGVDLSKIPLI
KNATGAQV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001040403
ORF Size 1284 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001040403.2
RefSeq Size 2627 bp
RefSeq ORF 1287 bp
Locus ID 14252
UniProt ID Q60634
Cytogenetics 11 46.74 cM
Summary May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001040403" in other vectors (3)
SKU Description Size Price
MR222238 Flot2 (Myc-DDK-tagged) - Mouse flotillin 2 (Flot2), transcript variant 1 10 ug
$289.00 $457.00
MR222238L3 Lenti ORF clone of Flot2 (Myc-DDK-tagged) - Mouse flotillin 2 (Flot2), transcript variant 1 10 ug
$757.00
MR222238L4 Lenti ORF clone of Flot2 (mGFP-tagged) - Mouse flotillin 2 (Flot2), transcript variant 1 10 ug
$757.00

Plasmid Map for MG222238

Download sequence for

Cloning scheme for MG222238

Circular map for MG222238

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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