Ctsa (NM_008906) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG220402
Ctsa (tGFP-tagged) - Mouse cathepsin A (Ctsa) transcript variant 1, (10ug)
  $932.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ctsa
Synonyms AU019505; P; Pp; PPCA; Ppgb
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG220402 representing NM_008906
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTTCCAGTCCAAAGGCGCCTCCTGGAGAGCAAGGACGCAAGGAAGCAGAGATGCCCGGAACCGCGC
TGTCTCCACTGCTCTTGTTGCTGCTCCTGTCCTGGGCGTCCCGGAACGAAGCAGCTCCGGACCAGGATGA
AATCGATTGTCTCCCCGGCCTGGCCAAGCAGCCCTCTTTCCGGCAATACTCCGGCTACCTCAGAGCATCG
GACTCCAAGCACTTCCACTACTGGTTTGTGGAGTCGCAGAACGACCCAAAGAACAGCCCCGTGGTGCTTT
GGCTTAACGGGGGTCCCGGCTGCAGCTCGCTCGATGGGCTGCTTACAGAGCACGGCCCCTTTCTGATCCA
GCCAGATGGTGTCACCCTGGAGTACAACCCCTATGCTTGGAACCTGATTGCCAACGTGCTGTATATCGAG
TCCCCAGCTGGGGTGGGCTTCTCCTACTCGGATGACAAGATGTACGTGACCAATGACACAGAGGTGGCGG
AGAACAATTATGAAGCCCTTAAAGACTTCTTCCGCCTCTTTCCGGAATACAAGGACAACAAACTTTTCCT
GACAGGAGAGAGCTATGCTGGCATCTACATCCCCACCTTGGCTGTACTGGTCATGCAGGATCCTAGCATG
AATCTTCAGGGGCTGGCTGTGGGCAATGGACTTGCCTCCTATGAGCAGAACGACAACTCCCTGGTCTACT
TTGCCTACTACCATGGCCTTCTGGGGAACAGACTTTGGACTTCACTGCAGACCCACTGCTGCGCTCAGAA
CAAGTGTAACTTCTATGACAACAAAGACCCAGAGTGTGTAAACAATCTCCTGGAAGTGTCTCGAATTGTG
GGCAAATCTGGCCTCAACATCTACAATCTCTATGCTCCGTGTGCTGGTGGGGTGCCCGGCAGACATAGAT
ATGAGGACACACTTGTAGTCCAGGATTTTGGCAACATCTTCACTCGCCTGCCACTTAAGCGGAGATTTCC
TGAGGCACTGATGCGTTCTGGGGACAAGGTACGCTTGGATCCTCCCTGCACCAACACCACAGCCCCTTCC
AACTACCTCAACAACCCCTATGTTCGGAAGGCTCTCCACATCCCCGAGTCGCTGCCCCGCTGGGACATGT
GCAACTTCTTGGTGAATTTACAGTACCGCCGCCTCTACCAAAGCATGAACTCCCAGTACCTGAAGCTGCT
CAGTTCACAGAAATACCAGATCCTGCTCTACAACGGAGATGTGGACATGGCCTGCAACTTCATGGGCGAT
GAGTGGTTTGTGGATTCGCTCAACCAGAAGATGGAGGTGCAGCGCCGGCCCTGGCTAGTGGACTACGGGG
AGAGCGGAGAACAGGTAGCTGGTTTCGTGAAGGAGTGTTCACACATCACCTTCCTCACCATCAAGGGTGC
CGGACACATGGTCCCCACGGACAAGCCTCGAGCTGCTTTTACCATGTTCTCGAGGTTCCTGAACAAAGAG
CCTTAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG220402 representing NM_008906
Red=Cloning site Green=Tags(s)

MTSSPKAPPGEQGRKEAEMPGTALSPLLLLLLLSWASRNEAAPDQDEIDCLPGLAKQPSFRQYSGYLRAS
DSKHFHYWFVESQNDPKNSPVVLWLNGGPGCSSLDGLLTEHGPFLIQPDGVTLEYNPYAWNLIANVLYIE
SPAGVGFSYSDDKMYVTNDTEVAENNYEALKDFFRLFPEYKDNKLFLTGESYAGIYIPTLAVLVMQDPSM
NLQGLAVGNGLASYEQNDNSLVYFAYYHGLLGNRLWTSLQTHCCAQNKCNFYDNKDPECVNNLLEVSRIV
GKSGLNIYNLYAPCAGGVPGRHRYEDTLVVQDFGNIFTRLPLKRRFPEALMRSGDKVRLDPPCTNTTAPS
NYLNNPYVRKALHIPESLPRWDMCNFLVNLQYRRLYQSMNSQYLKLLSSQKYQILLYNGDVDMACNFMGD
EWFVDSLNQKMEVQRRPWLVDYGESGEQVAGFVKECSHITFLTIKGAGHMVPTDKPRAAFTMFSRFLNKE
PY

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008906
ORF Size 1476 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008906.4, NP_032932.2
RefSeq Size 3674 bp
RefSeq ORF 1479 bp
Locus ID 19025
Cytogenetics 2 85.27 cM
Summary This gene encodes a glycoprotein with deamidase, esterase and carboxypeptidase activities. The encoded protein associates with and provides a protective function to the lysosomal enzymes beta-galactosidase and neuraminidase. Deficiency of the related gene in humans results in galactosialidosis. The proprotein is processed into two shorter chains. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. provided by RefSeq, Jan 2013
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Documents
Resources
"NM_008906" in other vectors (4)
SKU Description Size Price
MC216811 Ctsa (untagged) - Mouse cathepsin A (Ctsa), transcript variant 1, (10ug) 10 ug
$503.00
MR220402 Ctsa (Myc-DDK-tagged) - Mouse cathepsin A (Ctsa), transcript variant 1 10 ug
$732.00
MR220402L3 Lenti ORF clone of Ctsa (Myc-DDK-tagged) - Mouse cathepsin A (Ctsa), transcript variant 1 10 ug
$1,032.00
MR220402L4 Lenti ORF clone of Ctsa (mGFP-tagged) - Mouse cathepsin A (Ctsa), transcript variant 1 10 ug
$1,032.00

Plasmid Map for MG220402

Download sequence for

Cloning scheme for MG220402

Circular map for MG220402

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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