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Skint6 (NM_001103199) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG220125
Skint6 (tGFP-tagged) - Mouse selection and upkeep of intraepithelial T cells 6 (Skint6), (10ug). Note: ORF is codon optimized
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
  $1,387.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Skint6
Synonyms OTTMUSG00000008519
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG220125 representing NM_001103199, codon optimized.
Due to the complexity of NM_001103199, the ORF clone is codon optimized for mammalian Expression.
The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.

Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAACCATTGGGGTGCCTCTTACCGCACACTGTGTCGTGCTGTTTCTGCTTCAAATGGTCGCGCTGA
GCACCGAGCAGTTCACCGTTAACGGTCTTGAATCACCAGTGCTGGTACCTTTGGGTGGTAACCTGGAGCT
GAGCTGTCAGTTGTCCCCCCCACAGCAGGCCAAGCATATGGAGATTCGGTGGTTCAAGAATCGGTACAGC
GAGCCGGTATACCTTTACAGGAACGGAAAGGATTTGAACGAAGCCATAGTGTACAAGTATGTAGAGCGAA
CAGAGCTCCTCAAGGATGATATCGGCAAGGGTAAGGTGACTCTCAGAATTTTTAAAGTTACATCAGTTGA
CTCCGGATCCTACCACTGTTTTTTTAAGGATGATAAATTTTATGAAGAGCATATCATTGAAGTCAAAGTG
ACCGCTACATCTAGCGATATACAGATCCTGATGCACCCCCCTAATAGCACTGGGGTTAGGCTCGAATGCC
ACTCAGGCGGTTGGTTTCCACAACCCCATATGGAGTGGCGCGACTCCAAAGGCGAATACATCCCAGCTAC
CAGCAAGAGCCACTCTCAGGACGAGAATGAGTTCTTCAACATGACCATGGATCTGTTCATTAAGAGTAAC
AGTTACTGGAGCGCCGCCTGCTATATACAAAACTTTGTCACCCACCAAGAGGAAAGCATAAGCTTTGTGC
TGCCCGGGACAAAAGTACTGCTGCTCAAGAATATTCAAAGTATATTTCAGAGCTCCAGCGTGGATCTGGA
GAAGAAAGATATGCTTCTGAAGAGTATAAAATCACGCTTCCAATCATCATCAGTTGATCCAGAGAAGAAA
GAGCGGTTGCTGAAAATCATTCAGAGCAGAATCGAGAGCTCCTCCGTCGACCAGGAAACGAAAGCACTGT
TGCTGGAGTCTATCCAGTCTTCAATTGAAAATAGCACCGTGGATTTGGAGACGAAGGTATGGCTCCTGGA
ATCTATCAAGTCCAGTATTCAGAACTCTAGCGTAGATCCAGAAACTAAGGAATTGCTGTTGGAACGCATC
AAATCTTCTATCGAGAATTCCACCGTGGATCTGGGCACAAAGGAGTGGCTCCTCGAGAGTATACAGTCAA
GCATCCAATCAAGCAGCGTGGACCTGGGCACTAAAAAAATGCTCCTTAAGATCATACAGTCATCAATCCA
GTCATTTAGCGTATACATAGGAACCAAGGAATGGCTGTTGGAGAGGGTGCAGTCCAGCATCCAGTCCTCC
AGCGTCGACCTTGGCACAAAAGAACTCCTGCTGGAAATTATCCAAAGCAGCATCGAGGGCTATTCTGTCG
AGCTCGGCATCGAAGAGCTGCTTTCCAAGATTATCCTGAGTAGCATACAGTCCAGCCGCGTTGACCTCGG
GATCAAAGAGCTTCTGGTGAAGATCTGCCAGTCATCTATCCAGAGTACCTCAGTGATCCTTGAAACAAAA
GAATGGCTCGTCAAAATTTTCCAGTCCAACATTCAATCTTCATCAATGGACCTGGAGACAAAAGTGTGGC
TGGTCAAGTTCTTCCAGTCCAGCATTCAGTCCTCCTCAATGGATCTGGGTACTATCAAGTGGTTGGTCAA
GAACGTGCAATCCAACATACAAAGCTCCAGGGTGGATCTCGAGACAAAACGAATGTTGTTGGAGATCATC
CAGTCTAGTATTCAGAACAGCAGTGTGCATCGGGGAACCAAGAAAATGTTGCTCAAGGTGATTCAGTCAT
CCATTCAGTCTTTTAGCGTGGATCTGGAGACAAAAGAGTGGCTGCTGAAAATCATTCAGAGTTCAATCCA
AAGCAGCTCTGTAGATATTGGAACGAAAAGTATGCTCCTGAAGAAGATTGGCCTTATTCTGAGAAGCTCA
ATTGTGAACCCGGGCACGGAGCTCCTCTTCCAGATCATACAGTCTGATCTGCAGTCCTCCTCCGTGAATA
CAGAAACAAGGGAGATGCTGTTCGAGATTACACAGCCAACCGTCCAGTCATCCTCTGTGAACTCCGTGAC
TGAAGAACTGTTGGAAGAAAATTACCAACTGCTGCTGCAGTCCAGTAGTGTGAACTTGGAGGCAGAGAAT
ATCTTGAATGACACCACCCAGCGGATCCTGCAGCTTAGCAGTGTAAATCAGGGCCGAGAGAAACTGTTGC
TGGATAGGATCCAACAGATCCTGCAGTCTAGTTCCGTTAACCCTGAGACTAAATGCTGGCTGCTGAAACG
GATACAGTTTATCCTGGAAAACCCAAGCGTTCATCAGGAAAAGAAGGACCTGCTGCTTGAGCGGATTCAT
TTGATTCTGCAGTCAAGCTCAGTGCAGAAAGAGACAAAGTCTCTGCTGCTGGGTTCCATTCAGTCTATAC
TGCACTCATTCTCCGTTCAGAAGGAAACTAAATACCTCCTGCTTGAGAAGATTCAGCTGATACTCCAGAA
CAGCAGTGTTCATCAGGAGACAAAGGATCTGCTGCTGAAAATTATTCAGTTGATACTGCAGTACAGCTCT
GTCCATGAGGCGACGTCAAAATTGCTGTTGGAAAGGATACAGCTGATCCTCCAGTCTTCATCAGTCCAGA
AGGAAACAAAGGACCTGCTGCTCAATAGAATTTTCTCCATTTTGCAGAACTCATGCGTAGACCAAGGCGT
GAAGTATTTCCTGCTGAACATCATCCACCCTATCCTTCAGAAGACTTCTGTACACCAGGAAACAAAAAGT
ATGCTGCTGGATCGGATCGAATTGATCCTTCAGTCAAGCTCCATGCAGCAAGAAACTAAAAACCACCTCC
TTGACCAAATCGAGCAGATCCTCCAGGATACTACTGTGCATCAGGAGACAAAGGACCTGCTGCTCAATCG
CATTGAGCTGATTTTGCAGAATAGCTCTGTCCAGCAGGAGACTAAAGAGCTGCTCATGGATAAGATAGAC
TCTATTCTTCGCTACACTTGGGTGCACCAGGAAACAAAAGACCTCTTGCTGAATAGGATCGAGTTGATCC
TCCAAAGTAGCTCAGTTCAGGAAGAAACAAAGAACTTCCTGTGTAACATCATTGCCTCTATCCTCCAGAG
TACCTCCATGCACCAGGAAAAAAAAAAGTTGTTGCTGGGACGAATAGAACTTATTCTGCAGAGTAGCTCA
GTGCAGCAAGAGACAAAGAAACTCCTGCTGAAGATCGTCCAATCTAGTAGTCCCTCTAGCAGTGTGAGGC
TTGAAAGTTGCAATAAGAGGAACCCCTTTTGGAAGAAGCATGCCCTTGATTTGGGAATAAGTGTTTTCGC
TATCATCGTCGTCACACTCATACGCCATCTGAATCAGAGGGAGGCCGACCAACACTTCGAATTGGATACC
CTCTGGTCTAAAGATACCTCCGTTATCCTGTGTGTGCTCATCATGTTTAACAATAGGCTGAAAGCTCTCA
TCTATTTTAGGCTGTACGGCTACTCCCCTCCCGGGAAGACATACAAGTATATTGTTAACTACATATTGCG
CTTTAGCCAACCACTGTTCTTCATTGTATATTCAGCCATTATCCTTGTTATGCACCTGCAGATTCAGAAT
ACAGATTCCCTGTTCAGCCTGTACAATAGCTGGATGGTGGAGATGATCATGGTTCTCGGACTTTTGCTTG
CTATATTCAACGTCAAGAATATCGCAACCGCCCTGCTTCATCTGGGCCGCACCACCTTGCGCCTGTTTCG
GATCAAGGAT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG220125 representing NM_001103199
Red=Cloning site Green=Tags(s)

MGTIGVPLTAHCVVLFLLQMVALSTEQFTVNGLESPVLVPLGGNLELSCQLSPPQQAKHMEIRWFKNRYS
EPVYLYRNGKDLNEAIVYKYVERTELLKDDIGKGKVTLRIFKVTSVDSGSYHCFFKDDKFYEEHIIEVKV
TATSSDIQILMHPPNSTGVRLECHSGGWFPQPHMEWRDSKGEYIPATSKSHSQDENEFFNMTMDLFIKSN
SYWSAACYIQNFVTHQEESISFVLPGTKVLLLKNIQSIFQSSSVDLEKKDMLLKSIKSRFQSSSVDPEKK
ERLLKIIQSRIESSSVDQETKALLLESIQSSIENSTVDLETKVWLLESIKSSIQNSSVDPETKELLLERI
KSSIENSTVDLGTKEWLLESIQSSIQSSSVDLGTKKMLLKIIQSSIQSFSVYIGTKEWLLERVQSSIQSS
SVDLGTKELLLEIIQSSIEGYSVELGIEELLSKIILSSIQSSRVDLGIKELLVKICQSSIQSTSVILETK
EWLVKIFQSNIQSSSMDLETKVWLVKFFQSSIQSSSMDLGTIKWLVKNVQSNIQSSRVDLETKRMLLEII
QSSIQNSSVHRGTKKMLLKVIQSSIQSFSVDLETKEWLLKIIQSSIQSSSVDIGTKSMLLKKIGLILRSS
IVNPGTELLFQIIQSDLQSSSVNTETREMLFEITQPTVQSSSVNSVTEELLEENYQLLLQSSSVNLEAEN
ILNDTTQRILQLSSVNQGREKLLLDRIQQILQSSSVNPETKCWLLKRIQFILENPSVHQEKKDLLLERIH
LILQSSSVQKETKSLLLGSIQSILHSFSVQKETKYLLLEKIQLILQNSSVHQETKDLLLKIIQLILQYSS
VHEATSKLLLERIQLILQSSSVQKETKDLLLNRIFSILQNSCVDQGVKYFLLNIIHPILQKTSVHQETKS
MLLDRIELILQSSSMQQETKNHLLDQIEQILQDTTVHQETKDLLLNRIELILQNSSVQQETKELLMDKID
SILRYTWVHQETKDLLLNRIELILQSSSVQEETKNFLCNIIASILQSTSMHQEKKKLLLGRIELILQSSS
VQQETKKLLLKIVQSSSPSSSVRLESCNKRNPFWKKHALDLGISVFAIIVVTLIRHLNQREADQHFELDT
LWSKDTSVILCVLIMFNNRLKALIYFRLYGYSPPGKTYKYIVNYILRFSQPLFFIVYSAIILVMHLQIQN
TDSLFSLYNSWMVEMIMVLGLLLAIFNVKNIATALLHLGRTTLRLFRIKD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001103199
ORF Size 3720 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001103199.1, NP_001096669.1
RefSeq Size 3824 bp
RefSeq ORF 3723 bp
Locus ID 230622
UniProt ID A7XUZ6
Cytogenetics 4 D1
Summary May act by engaging a cell surface molecule on immature T-cells in the embryonic thymus.UniProtKB/Swiss-Prot Function
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"NM_001103199" in other vectors (2)
SKU Description Size Price
MC224041 Skint6 (untagged) - Mouse selection and upkeep of intraepithelial T cells 6 (Skint6), (10ug) 10 ug
$1,188.00
MR220125 Skint6 (Myc-DDK-tagged) - Mouse selection and upkeep of intraepithelial T cells 6 (Skint6). Note: ORF is codon optimized 10 ug
$1,187.00

Plasmid Map for MG220125

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG220125
Circular map for MG220125

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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