Eri3 (NM_080469) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG219956
Eri3 (tGFP-tagged) - Mouse exoribonuclease 3 (Eri3), (10ug)
  $657.00
3 Weeks*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Eri3
Synonyms PINT1; Prnpip1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG219956 representing NM_080469
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGACAGCCTCTCCGGCAGCTGACGGGGGGCGGGGGCGGCCCTGGGAAGGAGGGCTGGTCTCCTGGC
CCCCTGCCCCTCCCCTTACGCTTCCCTGGACCTGGATGGGCCCGAGCTGGGGGCAACACCCTGGGCATTG
GGGCTTTCCAGCTCTCACAGACCCTTCAGCATCTCCAGCTGCCAGTCTTGGCATCTTCGAAGTAAGGAGA
GTTTTAGATGCCTCTGGATGTTCAATGCTAGCGCCGTTACAGACGGGAGCGGCTCGGTTCTCTTCATATT
TACTTTCAAGAGCAAGAAAAGTGCTGGGCTCCCACTTGTTATCTCCCTGTGGCGTTCCGGAGCTCTGTTC
CATATCCACCAGAAAGCTGGCGGCCCACGGCTTTGGCGCGGCAATGGCGGCAATGGTGCCCTTCCCTCCC
CAGAGGTATCACTACTTTTTAGTGCTGGACTTTGAGGCCACATGCGACAAGCCACAGATCCACCCTCAGG
AAATCATTGAGTTCCCCATCCTGAAGTTAAATGGCCGGACCATGGAAATCGAGTCTACCTTTCACATGTA
TGTCCAGCCTGTAGTCCACCCACAGCTTACCCCCTTCTGTACAGAGCTCACCGGGATCATTCAAGCCATG
GTGGATGGCCAGCCAAGCCTGCAGCAAGTGCTGGAGAGGGTCGATGAGTGGATGGCGAAGGAAGGCCTCT
TAGATCCAAACGTCAAGTCAATCTTTGTCACCTGCGGAGACTGGGACCTGAAAGTCATGCTTCCAGGACA
GTGCCATTATCTAGGCTTGCCAGTGGCGGATTACTTCAAGCAGTGGATTAATCTAAAAAAGGCTTACAGC
TTCGCCATGGGCTGCTGGCCCAAGAATGGACTACTAGACATGAACAAGGGTCTCAGCCTGCAGCACATAG
GCCGGCCCCACAGCGGCATTGATGACTGCAAGAACATTGCCAACATCATGAAGACATTGGCATACCGAGG
CTTCATCTTCAAGCAGACATCAAAGCCCTTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG219956 representing NM_080469
Red=Cloning site Green=Tags(s)

MATASPAADGGRGRPWEGGLVSWPPAPPLTLPWTWMGPSWGQHPGHWGFPALTDPSASPAASLGIFEVRR
VLDASGCSMLAPLQTGAARFSSYLLSRARKVLGSHLLSPCGVPELCSISTRKLAAHGFGAAMAAMVPFPP
QRYHYFLVLDFEATCDKPQIHPQEIIEFPILKLNGRTMEIESTFHMYVQPVVHPQLTPFCTELTGIIQAM
VDGQPSLQQVLERVDEWMAKEGLLDPNVKSIFVTCGDWDLKVMLPGQCHYLGLPVADYFKQWINLKKAYS
FAMGCWPKNGLLDMNKGLSLQHIGRPHSGIDDCKNIANIMKTLAYRGFIFKQTSKPF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_080469
ORF Size 1011 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_080469.4, NP_536717.2
RefSeq Size 1574 bp
RefSeq ORF 1014 bp
Locus ID 140546
UniProt ID Q8C460
Cytogenetics 4 D1-D2.1
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Documents
Resources
"NM_080469" in other vectors (4)
SKU Description Size Price
MC212309 Eri3 (untagged) - Mouse exoribonuclease 3 (Eri3), (10ug) 10 ug
$503.00
MR219956 Eri3 (Myc-DDK-tagged) - Mouse exoribonuclease 3 (Eri3) 10 ug
$289.00 $457.00
MR219956L3 Lenti ORF clone of Eri3 (Myc-DDK-tagged) - Mouse exoribonuclease 3 (Eri3) 10 ug
$757.00
MR219956L4 Lenti ORF clone of Eri3 (mGFP-tagged) - Mouse exoribonuclease 3 (Eri3) 10 ug
$757.00

Plasmid Map for MG219956

Download sequence for

Cloning scheme for MG219956

Circular map for MG219956

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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