Bmp8a (NM_007558) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG219188
Bmp8a (tGFP-tagged) - Mouse bone morphogenetic protein 8a (Bmp8a), (10ug)
$489.00 $703.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Bmp8a
Synonyms Bmp7; Bmp7r1; O; OP-2; OP2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG219188 representing NM_007558
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTATGCGTCCCGGGCCACTCTGGCTATTGGGCCTTGCTCTGTGCGCGCTGGGAGGCGGCCACGGTC
CGCGTCCCCCGCACACCTGTCCCCAGCGTCGCCTGGGAGCGCGCGAGCGCCGCGACATGCAGCGTGAAAT
CCTGGCGGTGCTCGGGCTACCGGGACGGCCCCGACCCCGTGCACAACCCGCCGCTGCCCGGCAGCCAGCG
TCCGCGCCCCTCTTCATGTTGGACCTATACCACGCCATGACCGATGACGACGACGGCGGGCCACCACAGG
CTCACTTAGGCCGTGCCGACCTGGTCATGAGCTTCGTCAACATGGTGGAACGCGACCGTACCCTGGGCTA
CCAGGAGCCACACTGGAAGGAATTCCACTTTGACCTAACCCAGATCCCTGCTGGGGAGGCTGTCACAGCT
GCTGAGTTCCGGATCTACAAAGAACCCAGCACCCACCCGCTCAACACAACCCTCCACATCAGCATGTTCG
AAGTGGTCCAAGAGCACTCCAACAGGGAGTCTGACTTGTTCTTTTTGGATCTTCAGACGCTCCGATCTGG
GGACGAGGGCTGGCTGGTGCTGGACATCACAGCAGCCAGTGACCGATGGCTGCTGAACCATCACAAGGAC
CTGGGACTCCGCCTCTATGTGGAAACCGCGGATGGGCACAGCATGGATCCTGGCCTGGCTGGTCTGCTTG
GACGACAAGCACCACGCTCCAGACAGCCTTTCATGGTAACCTTCTTCAGGGCCAGCCAGAGTCCTGTGCG
GGCCCCTCGGGCAGCGAGACCACTGAAGAGGAGGCAGCCAAAGAAAACGAACGAGCTTCCGCACCCCAAC
AAACTCCCAGGGATCTTTGATGATGGCCACGGTTCCCGCGGCAGAGAGGTTTGCCGCAGGCATGAGCTCT
ACGTCAGCTTCCGTGACCTTGGCTGGCTGGACTGGGTCATCGCCCCCCAGGGCTACTCTGCCTATTACTG
TGAGGGGGAGTGTGCTTTCCCACTGGACTCCTGTATGAACGCCACCAACCATGCCATCTTGCAGTCTCTG
GTGCACCTGATGAAGCCAGATGTTGTCCCCAAGGCATGCTGTGCACCCACCAAACTGAGTGCCACCTCTG
TGCTGTACTATGACAGCAGCAACAATGTCATCCTGCGTAAACACCGTAACATGGTGGTCAAGGCCTGTGG
CTGCCAC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG219188 representing NM_007558
Red=Cloning site Green=Tags(s)

MAMRPGPLWLLGLALCALGGGHGPRPPHTCPQRRLGARERRDMQREILAVLGLPGRPRPRAQPAAARQPA
SAPLFMLDLYHAMTDDDDGGPPQAHLGRADLVMSFVNMVERDRTLGYQEPHWKEFHFDLTQIPAGEAVTA
AEFRIYKEPSTHPLNTTLHISMFEVVQEHSNRESDLFFLDLQTLRSGDEGWLVLDITAASDRWLLNHHKD
LGLRLYVETADGHSMDPGLAGLLGRQAPRSRQPFMVTFFRASQSPVRAPRAARPLKRRQPKKTNELPHPN
KLPGIFDDGHGSRGREVCRRHELYVSFRDLGWLDWVIAPQGYSAYYCEGECAFPLDSCMNATNHAILQSL
VHLMKPDVVPKACCAPTKLSATSVLYYDSSNNVILRKHRNMVVKACGCH

TRTRPLE - GFP Tag - V
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007558
ORF Size 1197 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007558.3, NP_031584.1
RefSeq Size 1917 bp
RefSeq ORF 1200 bp
Locus ID 12163
UniProt ID P34821
Cytogenetics 4 57.42 cM
Summary This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein may play a role in development of the reproductive system. Mice lacking a functional copy of this gene exhibit degeneration of germ cells and the epididymal epithelium. This gene may have arose from a gene duplication event and its gene duplicate is also present on chromosome 4. provided by RefSeq, Jul 2016
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Documents
Resources
"NM_007558" in other vectors (4)
SKU Description Size Price
MC208196 Bmp8a (untagged) - Mouse bone morphogenetic protein 8a (Bmp8a), (10ug) 10 ug
$503.00
MR219188 Bmp8a (Myc-DDK-tagged) - Mouse bone morphogenetic protein 8a (Bmp8a) 10 ug
$503.00
MR219188L3 Lenti ORF clone of Bmp8a (Myc-DDK-tagged) - Mouse bone morphogenetic protein 8a (Bmp8a) 10 ug
$803.00
MR219188L4 Lenti ORF clone of Bmp8a (mGFP-tagged) - Mouse bone morphogenetic protein 8a (Bmp8a) 10 ug
$803.00

Plasmid Map for MG219188

Download sequence for

Cloning scheme for MG219188

Circular map for MG219188

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