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Scrn3 (NM_029022) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216463
Scrn3 (tGFP-tagged) - Mouse secernin 3 (Scrn3), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Scrn3
Synonyms 4833415E20Rik; AI427019; AV002033; Ses3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216463 representing NM_029022
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAACCCTATTCCTGTGACACTTTTGTGGCATTACCTCCAGCAACAGTAGGAAATAGGGTTATTTTTG
GAAAAAATTCAGACAGACTCTTTGATGAAGTACAAGAAGTAATTTACTGTCCAGCTGCAGTTCATAATGA
TTTGGAAAAACGTCTCAAGTGTACTTATATAGAAGTTGATCAAGTTCCTGAAACATATGCTGTTGTCCTT
AGTCGCCCAGCTTGGCTGTGGGGGGCAGAAATGGGTGCCAATGAGCATGGAGTCTGCATTGGGAATGAGG
CTGTATGGGGAAGAGAAGACATTTCTAAGGAGGAAGCACTTTTGGGCATGGACCTTGTCAGGCTGGGCCT
TGAGAGAGCTGACACGGCTGAGAAAGCTCTGGATGTCATTGTTGACTTACTAGAAAAGTATGGCCAAGGT
GGAAACTGCGCAGAGGGCAAAGAGTTCAGCTATTACAACAGTTTCCTGATAGCTGACAGGAATGAAGCCT
GGATCCTGGAGACGTCGGGGAAGTACTGGGCAGCAGAGAGAGTCCAAGGCGTTCGTAATATTTCCAATCA
GCTTTCCATAACAACCAAGATTGACCGGGAACATCCAGACATGAGGAACTACGCTAAGCAGAGGGGCTGG
TGGGATGGCAAAGAAGAATTTGATTTTACTGCGGCCTATTCTTACATTGACACGGCTGCTATGATGACTT
CACCAAGCAGGTACTGCCAGGGCTACAAGCTTCTGGATAAACACAGAGGAAACATAACTTTTGAAACGAT
GATGGAAATTCTTCGAGACAGACCCAGTGGCATCAACATGAAGGGAGAATTCCTGACCACGGCAAGCATG
GTTTCTGTTTTACCTCAGGATCCCAGCCTTCCTTGCATTCACCTCTTTACTGCAACTCCGCACCCTGAGA
GGTCTGTTTTTAAGCCTTTCATATTTGTACCACACATTTCACCACTGTTGGATACAAAATCACCAACATT
TGAACCTGAAAGGCCAGTGGCAAAGAAGCCATATGTCAAGCCTGACAGAAGACACCCACTCTACCAAAAA
CATCAAGAGGCCTTGGAAATGATAAGTAACAGTAAGGAAAAAGGCAAGACAATATTGGACAAGATGAGGA
AGCTGGAAAAAGCAGTATCTGAAGAGATAGAATCAATCCTCCAGAGTGGACATCTCGACGAGGAGAAAAC
TGTTAATCTCTTTCCTCAATATGTGAAAGATGAAATTAAAATTTATCAGTCAAATATTAGTTCT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216463 representing NM_029022
Red=Cloning site Green=Tags(s)

MEPYSCDTFVALPPATVGNRVIFGKNSDRLFDEVQEVIYCPAAVHNDLEKRLKCTYIEVDQVPETYAVVL
SRPAWLWGAEMGANEHGVCIGNEAVWGREDISKEEALLGMDLVRLGLERADTAEKALDVIVDLLEKYGQG
GNCAEGKEFSYYNSFLIADRNEAWILETSGKYWAAERVQGVRNISNQLSITTKIDREHPDMRNYAKQRGW
WDGKEEFDFTAAYSYIDTAAMMTSPSRYCQGYKLLDKHRGNITFETMMEILRDRPSGINMKGEFLTTASM
VSVLPQDPSLPCIHLFTATPHPERSVFKPFIFVPHISPLLDTKSPTFEPERPVAKKPYVKPDRRHPLYQK
HQEALEMISNSKEKGKTILDKMRKLEKAVSEEIESILQSGHLDEEKTVNLFPQYVKDEIKIYQSNISS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_029022
ORF Size 1254 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_029022.1, NP_083298.1
RefSeq Size 3247 bp
RefSeq ORF 1257 bp
Locus ID 74616
UniProt ID Q3TMH2
Cytogenetics 2 C3
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Documents
Resources
"NM_029022" in other vectors (4)
SKU Description Size Price
MC211545 Scrn3 (untagged) - Mouse secernin 3 (Scrn3), (10ug) 10 ug
$503.00
MR216463 Scrn3 (Myc-DDK-tagged) - Mouse secernin 3 (Scrn3) 10 ug
$289.00 $457.00
MR216463L3 Lenti ORF clone of Scrn3 (Myc-DDK-tagged) - Mouse secernin 3 (Scrn3) 10 ug
$757.00
MR216463L4 Lenti ORF clone of Scrn3 (mGFP-tagged) - Mouse secernin 3 (Scrn3) 10 ug
$757.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.