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Vsig2 (NM_020518) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216309
Vsig2 (tGFP-tagged) - Mouse V-set and immunoglobulin domain containing 2 (Vsig2), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $500.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Vsig2
Synonyms 1190004B15Rik; 2210413P10Rik; AU040484; Ctm; CTX
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216309 representing NM_020518
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTGGCCCCTGGTGGGGGCTTTCCTTTGCGGGCACTTGCTGGGCTTCGTGTGCCTGAGCGGGCTAG
CTGTGGAGGTGACTGTGCCCACTGAGCCACTGAGCGTGCCAAAGGGTAAGACGGCCGAGCTGAGCTGCAG
TTACAAAACATCGGTGGGCGACAACTTTGCCTTGGAGTGGAGCTTTGTGCAGCCTGGGAAGCCCATCTCT
GCGTCCGTGCCCGTTCTGTACTTCACCAATGGCCACCTGTATCCCACTGGCTCTAAAGCAGATCGGGCCA
TCCTTCTCCACGATCCACCCACAGGAGGACTGGCTACCCTGAAACTGACTGACCTCCGCCCTTCAGACAC
TGGGACCTACCTCTGCAATGTTAACAACCCACCAGATTTCTACACCAACGGGTTGGGGTTAATCAACCTT
ACTGTGCTGGTGCCCCCCAGTCACCCCTTATGCAGCCAGAGTGGGCAAACCTCTGTGGGAGGTTCTGCTG
CACTGGGATGCCGCTCCTCTGAGGGAGCACCCAAGCCAGTGTACAACTGGGAACGTCTCGGATCTTCTCC
TACACCTCCTCCTGGTAGCATGGTTCAAGATGAAGTGTCCGGCCAGCTCATTCTTACCAATCTCTCCCTG
ACCTCCTCTGGCACCTACCGATGTGTGGCCAGCAACCAGATGGGCAGTGCCTCCTGTGAGCTGAACCTCT
CCGTGACTGACTCATCTGAAGGCCGAGTAGCTGGGACTCTTATTGGGGTGCTGCTGGGAGTGCTGCTCCT
GTCAGTCGCCGCATTCTGCCTCATCAGGTTCCAGAAAGAGAGGAAAAAGGAGCCCAAGGAGACCTATGGG
GGTAGTGACCTTCGGGAGGATGCAACCGCTCCTGGGGTTTTTGAGCAAGCTTCTATGAGAGCCGATCATA
GCAAAGAGCTCCTGGAGAAGTCTCCATGTGCCAGCACCATGACCACCACTAAGTCCAAACTCTCTATGGT
TGTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216309 representing NM_020518
Red=Cloning site Green=Tags(s)

MAWPLVGAFLCGHLLGFVCLSGLAVEVTVPTEPLSVPKGKTAELSCSYKTSVGDNFALEWSFVQPGKPIS
ASVPVLYFTNGHLYPTGSKADRAILLHDPPTGGLATLKLTDLRPSDTGTYLCNVNNPPDFYTNGLGLINL
TVLVPPSHPLCSQSGQTSVGGSAALGCRSSEGAPKPVYNWERLGSSPTPPPGSMVQDEVSGQLILTNLSL
TSSGTYRCVASNQMGSASCELNLSVTDSSEGRVAGTLIGVLLGVLLLSVAAFCLIRFQKERKKEPKETYG
GSDLREDATAPGVFEQASMRADHSKELLEKSPCASTMTTTKSKLSMVV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_020518
ORF Size 984 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020518.2, NP_065264.2
RefSeq Size 1170 bp
RefSeq ORF 987 bp
Locus ID 57276
UniProt ID Q9Z109
Cytogenetics 9 A4
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Documents
Resources
"NM_020518" in other vectors (4)
SKU Description Size Price
MC210134 Vsig2 (untagged) - Mouse V-set and immunoglobulin domain containing 2 (Vsig2), (10ug) 10 ug
$330.00
MR216309 Vsig2 (Myc-DDK-tagged) - Mouse V-set and immunoglobulin domain containing 2 (Vsig2) 10 ug
$289.00 $300.00
MR216309L3 Lenti ORF clone of Vsig2 (Myc-DDK-tagged) - Mouse V-set and immunoglobulin domain containing 2 (Vsig2) 10 ug
$600.00
MR216309L4 Lenti ORF clone of Vsig2 (mGFP-tagged) - Mouse V-set and immunoglobulin domain containing 2 (Vsig2) 10 ug
$600.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.