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Pigm (NM_026234) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG216263
Pigm (tGFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis class M (Pigm), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Pigm or NM_026234"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pigm
Synonyms 4933437L05Rik; AI644422; AV302080; C920011G20Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG216263 representing NM_026234
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCTACCCCATGCACTGGGGGGAATGGATCCTGAACTTTCGGGTGCCGCCCGCCGGGGTGTTCGGCG
TGGCCTTCCTGGCCCGGGTCGCCCTGGTTTTCTACGGTGTCTTCCAGGACCGGACTCTGCTGGTGAGGTA
CACCGACATAGACTACCACGTGTTCACGGACGCCGCGCGCTTCGTCACGGAAGGGCGCTCCCCGTACCTG
CGGGCGACCTACCGCTACACCCCGCTCCTGAGCTGGCTCCTCACCCCCAACGTCTACCTCAGCGAACTCT
TCGGCAAGTTTCTCTTCATCAGCTGCGATCTCCTCACCGCCTTCCTCCTGTACCGCCTGCTGCTGCTCAA
GGGGCTGGGGCGCCGCCAAGCCTGTGGCTACTGCGTCTTCTGGCTGCTCAACCCTTTGCCCATGGCGGTG
TCCAGCCGGGGCAACGCGGACTCCATCGTCGCCTCCCTGGTTCTCTCGACCCTGTACTTCATAGAGAAAC
GGCTCATCGCGTGCGCCGCGGTGTTCTATGGCTTCGCGGTGCACATGAAGATGTACCCGGTGACCTACAT
CCTCCCCATAGCGCTCCATCTGCGGCCCGAGCGCGACGATGACGAGCGTCTCCGGCATGCCCGGTTCTCT
TTCCAGGCTCGTTTGTACGACTTCCTGAGGAGGCTGTGTAGCTGGGCAGTGCTGCTTTTCGTAGCGGTTG
CAGGGCTCACGTTTGTTGCCCTGAGCTTCGGTTTTTACTATAAGTACGGTTGGGAGTTTTTGGAGCACAC
CTACTTTTATCACCTAACTAGGAGGGATATCCGCCATAATTTCTCTCCGTACTTCTATATGCTGTATTTG
ACTGCCGAGAGCAAGTGGAGTTTTACTCTGGGGATTGCGGCGTTCCTGCCACAGTTCATCTTGCTGTCAG
CCGCATCTTTCGCCTATTACAGAGACCTTGTCTTCTGCTGTTTCCTTCATACGTCCATTTTTGTGACTTT
TAACAAAGTCTGCACCTCTCAGTACTTTCTTTGGTACCTCTGCCTTCTGCCTCTGGTGATGCCACTGGTG
AGGATGCCTTGGAAAAGAGCCGTAGTCCTCTTACTGTTCTGGTTCATAGGACAGGCCTTATGGCTGGCTC
CTGCATATGTTCTAGAGTTTCAAGGAAAGAACACCTTCCTGTTTATTTGGTTAGCCGGTTTGTTCTTCCT
TCTGATCAACTGTTCCATACTGATTCAGATTATTTCCCATTACAAGGAGGACCGCCTAACGGAGAGAATC
AAATACGAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG216263 representing NM_026234
Red=Cloning site Green=Tags(s)

MSYPMHWGEWILNFRVPPAGVFGVAFLARVALVFYGVFQDRTLLVRYTDIDYHVFTDAARFVTEGRSPYL
RATYRYTPLLSWLLTPNVYLSELFGKFLFISCDLLTAFLLYRLLLLKGLGRRQACGYCVFWLLNPLPMAV
SSRGNADSIVASLVLSTLYFIEKRLIACAAVFYGFAVHMKMYPVTYILPIALHLRPERDDDERLRHARFS
FQARLYDFLRRLCSWAVLLFVAVAGLTFVALSFGFYYKYGWEFLEHTYFYHLTRRDIRHNFSPYFYMLYL
TAESKWSFTLGIAAFLPQFILLSAASFAYYRDLVFCCFLHTSIFVTFNKVCTSQYFLWYLCLLPLVMPLV
RMPWKRAVVLLLFWFIGQALWLAPAYVLEFQGKNTFLFIWLAGLFFLLINCSILIQIISHYKEDRLTERI
KYD

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026234
ORF Size 1269 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026234.4, NP_080510.1
RefSeq Size 7569 bp
RefSeq ORF 1272 bp
Locus ID 67556
UniProt ID Q8C2R7
Cytogenetics 1 79.73 cM
Summary Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the first alpha-1,4-mannose to GlcN-acyl-PI during GPI precursor assembly (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_026234" in other vectors (4)
SKU Description Size Price
MC202775 Pigm (untagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class M (Pigm), (10ug) 10 ug
$457.00
MR216263 Pigm (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class M (Pigm) 10 ug
$289.00 $457.00
MR216263L3 Lenti ORF clone of Pigm (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class M (Pigm) 10 ug
$757.00
MR216263L4 Lenti ORF clone of Pigm (mGFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class M (Pigm) 10 ug
$757.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.