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Col19a1 (NM_007733) Mouse Tagged ORF Clone

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SKU
MG215958
Col19a1 (tGFP-tagged) - Mouse collagen type XIX alpha 1 (Col19a1), (10ug)
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ORF Plasmid:
tGFP DDK
  $1,190.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Col19a1 or NM_007733"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Col19a1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215958 representing NM_007733
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGCACACTGGCTCTTGGAAATTGTGGACCTGGGTGACAACCTTCCTGCTTCCTGCATGTACCTGTC
TGACAGTCAGGGACAAGCCAGAAACAACCTGCCCTACACTGAGAACAGAGAGATATCAAGATGACAGAAA
CAAATCTGAATTGTCAGGCTTTGATCTGGGAGAAAGCTTTGCTCTGAGGCATGCGTTTTGCGAAGGTGAT
AAAACCTGTTTCAAGTTGGGAAGTGTGCTTCTTATCAGAGACACTGTTAAGATATTTCCCAAAGGCCTTC
CAGAGGAATACGCTATAGCTGTCATGTTTCGGGTACGAAGAAGCACCAAAAAGGAACGGTGGTTTTTATG
GAAGATTTTAAACCAGCAGAATATGGCCCAGATATCTGTAGTGATTGATGGTACGAAGAAGGTGGTAGAA
TTTATGTTCCGAGGTGCTGAGGGAGATCTGTTGAACTATGTTTTTAAGAATCGAGAACTCCGTCCACTCT
TTGATCGTCAATGGCATAAACTTGGCATTGGCGTGCAATCCCGAGTCCTTTCTCTCTATATGGATTGCAA
TTTAATTGCAAGTCGACACACGGAAGAAAAGAATTCTGTGGATTTTCAAGGAAGGACAATTATTGCTGCA
CGAGCTTCAGATGGCAAACCTGTGGATATCGAACTTCACCAACTTAGAATCTACTGCAATGCAAACTTCC
TAGCTGAAGAATCATGTTGCAACTTATCACCTACTAAGTGTCCAGAGCAAGACGACTTTGGAAGTACTAC
TTCATCCTGGGGGACCTCTAACACTGGTAAAATGTCTTCATATCTGCCAGGAAAGCAGGAACTTAAAGAC
ACATGCCAGTGTATTCCAAACAAGGAGGAAGCAGGATTACCAGGAACTCTCAGATCCATTGGACATAAGG
GCGACAAAGGAGAGCCGGGTGAACATGGCTTAAATGGTACTCCAGGATTACCTGGTCAAAAGGGAGAACA
AGGACTTGAAGGCATCAAAGGAGAAATTGGTGAAAAGGGTGAACCAGGAGCCAAAGGAGACTCTGGTCTG
GATGGCCTCAATGGACAAGATGGTTTGAAAGGTGACTCGGGTCCTCAAGGTCCACCTGGCCCAAAAGGAG
ACAAGGGTGATATGGGACCTCCAGGGCCACCAGCCTTAACTGGCTCCATAGGAATCCAAGGTCCCCAGGG
TCCACCTGGCAAAGAAGGCCAAAGGGGAAGACGAGGGAAAACAGGACCCCCAGGAAATCCAGGACCTCCA
GGACCACCCGGACCTCCTGGGTTACAAGGACTACAGCAGCCTTTTGGTGGATATTTCAATAAGGGAACTG
GTGAGCACGGAGCTAGTGGCCCCAAAGGAGAAAAGGGCGATACTGGGCTGCCTGGATTTCCAGGGTCTGT
TGGCCCTAAAGGACACAAAGGAGAACCTGGGGAGCCTTTAACAAAAGGTGAAAAGGGAGATAGAGGAGAA
CCCGGCCTATTAGGACCACAGGGAATAAAGGGGGAACCTGGAGATCCTGGTCCCCCTGGTTTACTAGGAA
GCCCAGGGCTAAAGGGTCAGCAAGGACCTGCAGGTTCCATGGGGCCCAGAGGACCACCAGGAGATGTTGG
ATTGCCAGGAGAACATGGTATTCCAGGAAAACAAGGCGTTAAGGGAGAGAAGGGAGATCCGGGTGGAAGG
CTAGGCCCTCCTGGACTTCCAGGTCTAAAGGGTGATGCTGGGCCTCCAGGGATAAGCCTGCCAGGGAAAC
CAGGGTTAGATGGAAATCCAGGATCACCTGGTCCACGTGGGCCAAAGGGTGAAAGAGGTCTGCCTGGGCT
CCACGGTTCCCCCGGGGACACAGGCCCACCAGGTGTAGGAATCCCTGGTAGAACAGGCTCCCAAGGACCA
GCCGGAGAGCCAGGTATTCAGGGTCCTCGAGGTCTGCCTGGATTGCCAGGAACTCCAGGGATGCCAGGGA
ATGACGGAGCTCCAGGGAAGGATGGAAAGCCAGGCCTTCCAGGCCCCCCAGGTGACCCGATCGCACTTCC
TCTCTTGGGAGACATTGGCGCCCTGCTGAAGAACTTCTGTGGCAACTGTCAAGCCAATGTCCCTGGGCTG
AAAAGCATCAAAGGCGATGACGGAAGCACTGGGGAGCCTGGAAAGTATGAGCCTGCAGCCAGGAAGGGTG
ATGTAGGGCCACGAGGTCCTCCGGGATTCCCAGGCAGAGAAGGGCCAAAGGGAAGCAAAGGAGAGCGGGG
CTACCCTGGAATACACGGGGAGAAAGGCGATGAGGGTCTTCAAGGAATCCCTGGCCTCTCGGGAGCTCCT
GGCCCAACTGGACCCCCTGGCTTAACAGGAAGAACTGGACATCCTGGTCCCACTGGAGCAAAAGGTGACA
AGGGTAGTGAGGGACCCCCTGGGAAACCTGGACCTCCTGGACCCCCTGGTGTCCCGCTCAATGAGGGAAA
CGGCATGAGCAGTTTATATAAAATCCAGGGAGGGGTGAATGTTCCCGGTTACCCAGGACCTCCGGGCCCC
CCAGGTCCAAAAGGTGATCCTGGCCCAGTGGGAGAGCCTGGTGCAATGGGCTTGCCAGGACTAGAAGGAT
TTCCTGGTGTAAAGGGAGATCGAGGCCCAGCAGGCCCCCCAGGCATAGCCGGCATATCGGGAAAACCAGG
TGCCCCAGGCCCTCCAGGAGTACCAGGGGAGCAGGGTGAAAGAGGACCTATTGGAGATACAGGTTTTCCT
GGACCAGAGGGACCCTCGGGGAAGCCAGGCATAAATGGAAAAGATGGATTACCAGGTGCTCAGGGCATCA
TGGGTAAACCTGGTGACAGAGGCCCCAAAGGAGAACGCGGTGATCAGGGAATTCCAGGAGACAGAGGCCC
TCAAGGTGAACGTGGGAAGCCAGGTCTTACGGGCATGAAGGGGGCTATAGGCCCTGTGGGACCAGCAGGA
AGCAAGGGCTCCACTGGGCCACCTGGCCACCAAGGCCCTCCAGGCAATCCTGGCATCCCTGGCACTCCGG
CTGATGCTGTTTCATTTGAAGAAATAAAACACTACATTAATCAAGAAGTCCTAAGGATTTTTGAAGAGAG
GATGGCTGTGTTTCTATCCCAGCTTAAACTGCCAGCAGCAATGTTGTCTGCTCAAGCTCATGGGAGGCCT
GGCCCACCAGGGAAGGACGGGTTACCTGGACCCCCAGGAGATCCAGGGCCCCAAGGCTACCGAGGACAGA
AGGGAGAAAGAGGTGAACCTGGAATTGGGCTGCCGGGGAGTCCGGGTCTTCCTGGATCTTCAGCAGTGGG
TTTGCCGGGCTCACCAGGTGCCCCGGGGCCTCAAGGACCCCCGGGACCCAGTGGAAGATGTAATCCAGAA
GATTGCCTCTATCCTGCACCTCCTCCCCACCAGCAGGCAGGCGGGAAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215958 representing NM_007733
Red=Cloning site Green=Tags(s)

MRHTGSWKLWTWVTTFLLPACTCLTVRDKPETTCPTLRTERYQDDRNKSELSGFDLGESFALRHAFCEGD
KTCFKLGSVLLIRDTVKIFPKGLPEEYAIAVMFRVRRSTKKERWFLWKILNQQNMAQISVVIDGTKKVVE
FMFRGAEGDLLNYVFKNRELRPLFDRQWHKLGIGVQSRVLSLYMDCNLIASRHTEEKNSVDFQGRTIIAA
RASDGKPVDIELHQLRIYCNANFLAEESCCNLSPTKCPEQDDFGSTTSSWGTSNTGKMSSYLPGKQELKD
TCQCIPNKEEAGLPGTLRSIGHKGDKGEPGEHGLNGTPGLPGQKGEQGLEGIKGEIGEKGEPGAKGDSGL
DGLNGQDGLKGDSGPQGPPGPKGDKGDMGPPGPPALTGSIGIQGPQGPPGKEGQRGRRGKTGPPGNPGPP
GPPGPPGLQGLQQPFGGYFNKGTGEHGASGPKGEKGDTGLPGFPGSVGPKGHKGEPGEPLTKGEKGDRGE
PGLLGPQGIKGEPGDPGPPGLLGSPGLKGQQGPAGSMGPRGPPGDVGLPGEHGIPGKQGVKGEKGDPGGR
LGPPGLPGLKGDAGPPGISLPGKPGLDGNPGSPGPRGPKGERGLPGLHGSPGDTGPPGVGIPGRTGSQGP
AGEPGIQGPRGLPGLPGTPGMPGNDGAPGKDGKPGLPGPPGDPIALPLLGDIGALLKNFCGNCQANVPGL
KSIKGDDGSTGEPGKYEPAARKGDVGPRGPPGFPGREGPKGSKGERGYPGIHGEKGDEGLQGIPGLSGAP
GPTGPPGLTGRTGHPGPTGAKGDKGSEGPPGKPGPPGPPGVPLNEGNGMSSLYKIQGGVNVPGYPGPPGP
PGPKGDPGPVGEPGAMGLPGLEGFPGVKGDRGPAGPPGIAGISGKPGAPGPPGVPGEQGERGPIGDTGFP
GPEGPSGKPGINGKDGLPGAQGIMGKPGDRGPKGERGDQGIPGDRGPQGERGKPGLTGMKGAIGPVGPAG
SKGSTGPPGHQGPPGNPGIPGTPADAVSFEEIKHYINQEVLRIFEERMAVFLSQLKLPAAMLSAQAHGRP
GPPGKDGLPGPPGDPGPQGYRGQKGERGEPGIGLPGSPGLPGSSAVGLPGSPGAPGPQGPPGPSGRCNPE
DCLYPAPPPHQQAGGK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007733
ORF Size 3408 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007733.2, NP_031759.2
RefSeq Size 10542 bp
RefSeq ORF 3411 bp
Locus ID 12823
UniProt ID Q0VF58
Cytogenetics 1 A5
Summary May act as a cross-bridge between fibrils and other extracellular matrix molecules. Involved in skeletal myogenesis in the developing esophagus. May play a role in organization of the pericellular matrix or the sphinteric smooth muscle.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_007733" in other vectors (4)
SKU Description Size Price
MC223679 Col19a1 (untagged) - Mouse collagen, type XIX, alpha 1 (Col19a1), (10ug) 10 ug
$1,089.00
MR215958 Col19a1 (Myc-DDK-tagged) - Mouse collagen, type XIX, alpha 1 (Col19a1) 10 ug
$589.00 $990.00
MR215958L3 Lenti ORF clone of Col19a1 (Myc-DDK-tagged) - Mouse collagen, type XIX, alpha 1 (Col19a1) 10 ug
$1,290.00
MR215958L4 Lenti ORF clone of Col19a1 (mGFP-tagged) - Mouse collagen, type XIX, alpha 1 (Col19a1) 10 ug
$1,290.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.