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Heph (NM_010417) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG215386
Heph (tGFP-tagged) - Mouse hephaestin (Heph) transcript variant 1, (10ug)
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ORF Plasmid:
tGFP DDK
  $1,207.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Heph or NM_010417"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Heph
Synonyms C130006F04Rik; Cpl; mKIAA0698; sla
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG215386 representing NM_010417
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGCAGGCCATCTTCTCTGGGCTTTACTGTTGATGCACTCCTTGTGGTCTATACCAACTGATGGGG
CCATTCGAAACTACTACTTGGGCATCCAGGATATGCAGTGGAACTATGCTCCCAAAGGAAGAAATGTCAT
CACAAATCAGACTCTCAACAATGACACAGTGGCTTCCAGTTTCCTGAAGTCTGGCAAAAACAGGATAGGG
AGTAGTTACAAGAAGACTGTTTATAAGGAATACAGTGATGGCACATACACTGAAGAAATAGCCAAGCCTG
CCTGGTTGGGCTTCCTAGGACCACTGTTACAGGCTGAGGTGGGGGATGTCATCTTGATTCACCTGAAGAA
TTTTGCGAGCCGACCTTACACCATTCACCCTCACGGTGTTTTTTATGAGAAGGACTCAGAAGGCTCACTA
TACCCAGATGGTTCTTCTGGGTATCTGAAAGCGGATGATTCTGTCCCCCCTGGGGGCAGCCATGTCTACA
ACTGGAGTATTCCAGAAAGTCATGCCCCCACTGAGGCAGACCCAGCATGCCTCACCTGGATTTACCACTC
GCATGTAGATGCTCCAAGAGACATTGCAACTGGTCTCATTGGACCTCTTATCACCTGTAAAAGAGGGACC
CTGGATGGTAATTCCCCACCTCAGAGGAAGGATGTGGACCATAATTTCTTCCTCCTCTTCAGTGTGATAG
ATGAGAACCTTAGCTGGCACCTTGATGACAACATTGCTACTTACTGCTCAGACCCTGCCTCGGTGGACAA
AGAAGATGGAGCCTTTCAAGACAGCAACAGGATGCATGCAATCAATGGGTTTGTCTTTGGGAACTTACCA
GAGTTGAGCATGTGTGCACAGAAGCATGTGGCCTGGCACTTGTTTGGCATGGGCAATGAAATAGATGTCC
ACACAGCTTTCTTCCATGGACAGATGCTGAGTATCCGTGGACACCACACTGATGTTGCAAACATTTTTCC
AGCTACCTTTGTGACTGCTGAGATGGTGCCCCAAAAGTCTGGAACCTGGCTAATTAGCTGTGAAGTGAAC
AGCCACTTGAGAAGTGGCATGCAGGCCTTCTACAAGGTTGACTCTTGCTCCATGGACCCACCTGTGGACC
AGCTCACTGGCAAAGTTCGTCAGTACTTCATTCAGGCCCATGAGATTCAATGGGACTATGGTCCAATAGG
GTATGATGGCAGAACTGGGAAGAGTTTGAGAGAGCCAGGAAGTGGCCCAGATAAGTACTTCCAGAAGAGC
TCTAGTCGAATTGGAGGTACTTACTGGAAAGTTCGATATGAAGCCTTTCAAGATGAGACATTCCAGGAAA
GGGTACATCAGGAAGAAGAAACACATCTTGGAATACTGGGACCAGTGATAAGGGCTGAAGTGGGTGACAC
CATCCAGGTGGTCTTCTATAACCGTGCCTCCCAGCCATTCAGCATACAGCCCCATGGTGTCTTTTATGAG
AAAAACTCTGAGGGCACCGTGTACAATGATGGCACATCTCATCCCAAAGTAGCCAAGTCATTTGAAAAAG
TCACATACTACTGGACGGTTCCTCCCCATGCTGGGCCCACTGCTCAGGATCCTGCCTGTCTAACCTGGAT
GTACTTCTCTGCTGCAGATCCCACAAGAGATACAAATTCTGGCCTGGTGGGCCCTCTACTGGTGTGCAAG
GCTGGGGCCTTGGGTGCAGATGGCAAGCAGAAAGGAGTGGATAAAGAATTTTTTCTCCTCTTCACTGTGT
TTGATGAGAATGAGAGCTGGTACAACAGTGCCAATCAGGCAGCTGGTATGTTGGATTCCCGACTGCTCTC
AGAGGATGTCGAGGGCTTCCAGGACTCCAATCGAATGCATGCTATTAATGGATTTCTGTTCTCTAACCTG
CCCAGGCTGGACATGTGCAAGGGTGATACTGTGGCCTGGCACCTGCTTGGCCTGGGCACAGAGACTGATG
TACATGGGGTAATGTTCGAGGGCAACACTGTGCAGCTTCAGGGCATGAGGAAAGGTGCAGTCATGCTCTT
TCCTCACACCTTTGTGACGGCCATCATGCAGCCTGACAATCCTGGAATATTTGAAATCTACTGCCAAGCA
GGCAGCCACCGAGAGGAAGGGATGCAGGCAATTTATAATGTCTCTCAGTGTTCTAGTCATCAAGACAGCC
CACGCCAACACTACCAAGCTTCAAGAGTCTACTATATCATGGCAGAAGAGATAGAGTGGGATTACTGCCC
TGATAGAAGCTGGGAACTGGAATGGCATAACACATCTGAGAAAGACAGCTATGGCCATGTTTTCCTGAGC
AATAAAGATGGGCTCCTGGGTTCCAAATATAAGAAAGCTGTATTCAGGGAATACACTGATGGTACTTTCA
GAATACCTCGGCCAAGGTCTGGACCAGAGGAGCACTTGGGAATCCTGGGTCCACTTATCAGAGGAGAGGT
TGGTGATATCTTGACTGTAGTGTTCAAGAATAAGGCCAGTCGACCATATTCTATACATGCCCATGGAGTT
CTAGAATCTAACACTGGCGGGCCACAGGCTGCTGAGCCTGGTGAAGTACTTACTTACCAGTGGAACATCC
CAGAAAGATCTGGTCCTGGTCCTAGTGACTCTGCTTGTGTTTCCTGGATTTATTATTCTGCAGTGGATCC
CATCAAGGACATGTATAGTGGTCTGGTTGGACCCCTAGTCATCTGCAGAAATGGTATCTTGGAACCCAAT
GGAGGCCGGAATGATATGGACCGGGAATTTGCCTTGTTGTTTTTGATCTTTGATGAGAACCAATCTTGGT
ATCTGAAGGAGAATATTGCAACATATGGACCTCAAGAATCAAGTCATGTTAACTTGAAGGATGCCACCTT
CCTAGAGAGCAATAAAATGCATGCTATCAATGGGAAACTCTATGCAAACCTCAGGGGTCTTACTGTATAC
CAAGGAGAACGAGTAGCCTGGTACATGCTAGCCATGGGCCAAGATACTGACATTCACACTGTACACTTCC
ATGCAGAGAGTTTCCTCTATCAGAATGGGCAAAGTTACAGGGCAGATGTGGTGGATCTCTTCCCAGGAAC
ATTTGAAGTTGTGGAGATGGTAGCCAGCAACCCTGGGACATGGCTGATGCACTGCCATGTGACTGACCAT
GTTCATGCTGGCATGGAGACCATCTTTACGGTCTTGTCTCATGAAGAACATTTCAGCACTATGACCACTA
TTACTAAAGAGATTGGAAAAGTGATTCTAAGGGACATTGGAGGTGACAATGTGAAGATGCTGGGCATGAA
CATTCCCATAAAGGATGTAGAGATTCTGTCTTCTGCTTTGATTGCCATATGTGTGCTTCTGTTGCTCATT
GCTCTGGCTCTTGGTGGTGTAGTCTGGTACCAGCATCGACAAAGAAAGCTTCGGCGCAACAGGAGGTCCA
TTCTTGATGATAGCTTCAAGCTTCTCTCTCTCAAGCAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG215386 representing NM_010417
Red=Cloning site Green=Tags(s)

MKAGHLLWALLLMHSLWSIPTDGAIRNYYLGIQDMQWNYAPKGRNVITNQTLNNDTVASSFLKSGKNRIG
SSYKKTVYKEYSDGTYTEEIAKPAWLGFLGPLLQAEVGDVILIHLKNFASRPYTIHPHGVFYEKDSEGSL
YPDGSSGYLKADDSVPPGGSHVYNWSIPESHAPTEADPACLTWIYHSHVDAPRDIATGLIGPLITCKRGT
LDGNSPPQRKDVDHNFFLLFSVIDENLSWHLDDNIATYCSDPASVDKEDGAFQDSNRMHAINGFVFGNLP
ELSMCAQKHVAWHLFGMGNEIDVHTAFFHGQMLSIRGHHTDVANIFPATFVTAEMVPQKSGTWLISCEVN
SHLRSGMQAFYKVDSCSMDPPVDQLTGKVRQYFIQAHEIQWDYGPIGYDGRTGKSLREPGSGPDKYFQKS
SSRIGGTYWKVRYEAFQDETFQERVHQEEETHLGILGPVIRAEVGDTIQVVFYNRASQPFSIQPHGVFYE
KNSEGTVYNDGTSHPKVAKSFEKVTYYWTVPPHAGPTAQDPACLTWMYFSAADPTRDTNSGLVGPLLVCK
AGALGADGKQKGVDKEFFLLFTVFDENESWYNSANQAAGMLDSRLLSEDVEGFQDSNRMHAINGFLFSNL
PRLDMCKGDTVAWHLLGLGTETDVHGVMFEGNTVQLQGMRKGAVMLFPHTFVTAIMQPDNPGIFEIYCQA
GSHREEGMQAIYNVSQCSSHQDSPRQHYQASRVYYIMAEEIEWDYCPDRSWELEWHNTSEKDSYGHVFLS
NKDGLLGSKYKKAVFREYTDGTFRIPRPRSGPEEHLGILGPLIRGEVGDILTVVFKNKASRPYSIHAHGV
LESNTGGPQAAEPGEVLTYQWNIPERSGPGPSDSACVSWIYYSAVDPIKDMYSGLVGPLVICRNGILEPN
GGRNDMDREFALLFLIFDENQSWYLKENIATYGPQESSHVNLKDATFLESNKMHAINGKLYANLRGLTVY
QGERVAWYMLAMGQDTDIHTVHFHAESFLYQNGQSYRADVVDLFPGTFEVVEMVASNPGTWLMHCHVTDH
VHAGMETIFTVLSHEEHFSTMTTITKEIGKVILRDIGGDNVKMLGMNIPIKDVEILSSALIAICVLLLLI
ALALGGVVWYQHRQRKLRRNRRSILDDSFKLLSLKQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010417
ORF Size 3468 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010417.2, NP_034547.2
RefSeq Size 4693 bp
RefSeq ORF 3474 bp
Locus ID 15203
UniProt ID Q9Z0Z4
Cytogenetics X 42.69 cM
Summary May function as a ferroxidase for ferrous (II) to ferric ion (III) conversion and may be involved in copper transport and homeostasis. Implicated in iron homeostasis and may mediate iron efflux associated to ferroportin 1.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_010417" in other vectors (4)
SKU Description Size Price
MC223763 Heph (untagged) - Mouse hephaestin (Heph), transcript variant 1, (10ug) 10 ug
$1,109.00
MR215386 Heph (Myc-DDK-tagged) - Mouse hephaestin (Heph), transcript variant 1 10 ug
$589.00 $1,007.00
MR215386L3 Lenti ORF clone of Heph (Myc-DDK-tagged) - Mouse hephaestin (Heph), transcript variant 1 10 ug
$1,307.00
MR215386L4 Lenti ORF clone of Heph (mGFP-tagged) - Mouse hephaestin (Heph), transcript variant 1 10 ug
$1,307.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.