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Spryd3 (NM_001033277) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG214663
Spryd3 (tGFP-tagged) - Mouse SPRY domain containing 3 (Spryd3), (10ug)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Other products for "Spryd3 or NM_001033277"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Spryd3
Synonyms BC008150
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG214663 representing NM_001033277
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGAGGACGCGGCGGCCCCGGTTTGTTCTCATGAACAAGATGGATGACCTCAACTTGCACTACCGGT
TTCTGAACTGGCGAAGGAGGATTCGGGAGATCCGAGAGGTCCGGGCCTTCCGATATCAGGAGAGGTTCAA
GCATATCCTTGTAGATGGTGACACTTTGAGTTACCATGGAAATTCTGGTGAAGTTGGCTGCTACGTGGCG
TCTCGACCCCTGACCAAGGACAGCAATTATTTTGAGGTATCTATCGTGGACAGTGGCGTCCGGGGCACCA
TTGCTGTGGGGCTGGTCCCTCAGTACTACAGCTTGGATCACCAGCCTGGCTGGTTGCCTGACTCCGTAGC
CTACCATGCTGATGATGGCAAGCTGTACAACGGCCGAGCCAAGGGGCGCCAGTTCGGGTCAAAGTGCAAC
TCTGGGGACCGGATCGGCTGTGGCATTGAGCCTGTGTCCTTTGATGTGCAGACTGCCCAGATCTTCTTCA
CCAAAAATGGGAAACGGGTGGGCTCCACCATCATGCCCATGTCCCCAGATGGGCTCTTCCCAGCAGTTGG
TATGCATTCCCTGGGCGAGGAGGTAAGGCTGCACCTCAATGCCGAGCTGGGCCGTGAGGATGACAGCGTC
ATGATGGTGGACAGTTACGAGGATGAATGGGGCCGGCTGCATGACGTCAGAGTCTGTGGGACGCTGCTGG
AGTATTTGGGGAAGGGCAAGAGCATCGTGGATGTGGGGCTGGCTCAGGCTCGGCACCCACTGAGCACCCG
CAGCCATTACTTTGAGGTGGAGATTGTGGATCCTGGAGAGAAGTGCTATATCGCCTTGGGGCTGGCCCGG
AAGGATTATCCCAAGAACAGACACCCTGGCTGGAGCAGAGGGTCTGTGGCCTACCACGCAGATGACGGGA
AGATCTTCCATGGCAGTGGTGTGGGGGACCCCTTTGGGCCACGCTGCTACAAAGGAGACATTATGGGCTG
TGGAATCATGTTCCCCCGGGACTACATTCTGGACAGCGAGGGGGATAGTGATGACAGTTGTGACACAGTG
ATCTTGTCCCCGACTGCCCGGGCTGTCCGGAACGTCCGAAATGTCATGTACCTGCACCAGGAAGGGGAAG
AGGAAGAGGAGGAAGAGGAGGAAGAGGAGGATGGGGAAGAGATCGAGCAGGAGCATGAGGGCAAGAAGGT
GGTGGTCTTCTTCACTCGGAATGGCAAGATCATCGGGAAGAAGGATGCTGTTGTGCCGTCTGGAGGTTTC
TTTCCTACCATTGGCATGCTCAGTTGTGGGGAGAAAGTGAAAGTAGACCTGCACCCCTTGAGTGGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG214663 representing NM_001033277
Red=Cloning site Green=Tags(s)

MRRTRRPRFVLMNKMDDLNLHYRFLNWRRRIREIREVRAFRYQERFKHILVDGDTLSYHGNSGEVGCYVA
SRPLTKDSNYFEVSIVDSGVRGTIAVGLVPQYYSLDHQPGWLPDSVAYHADDGKLYNGRAKGRQFGSKCN
SGDRIGCGIEPVSFDVQTAQIFFTKNGKRVGSTIMPMSPDGLFPAVGMHSLGEEVRLHLNAELGREDDSV
MMVDSYEDEWGRLHDVRVCGTLLEYLGKGKSIVDVGLAQARHPLSTRSHYFEVEIVDPGEKCYIALGLAR
KDYPKNRHPGWSRGSVAYHADDGKIFHGSGVGDPFGPRCYKGDIMGCGIMFPRDYILDSEGDSDDSCDTV
ILSPTARAVRNVRNVMYLHQEGEEEEEEEEEEEDGEEIEQEHEGKKVVVFFTRNGKIIGKKDAVVPSGGF
FPTIGMLSCGEKVKVDLHPLSG

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001033277
ORF Size 1326 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033277.3, NP_001028449.2
RefSeq Size 2454 bp
RefSeq ORF 1329 bp
Locus ID 223918
Cytogenetics 15 F2
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Documents
Resources
"NM_001033277" in other vectors (4)
SKU Description Size Price
MC215868 Spryd3 (untagged) - Mouse SPRY domain containing 3 (Spryd3), (10ug) 10 ug
$503.00
MR214663 Spryd3 (Myc-DDK-tagged) - Mouse SPRY domain containing 3 (Spryd3) 10 ug
$289.00 $457.00
MR214663L3 Lenti ORF clone of Spryd3 (Myc-DDK-tagged) - Mouse SPRY domain containing 3 (Spryd3) 10 ug
$757.00
MR214663L4 Lenti ORF clone of Spryd3 (mGFP-tagged) - Mouse SPRY domain containing 3 (Spryd3) 10 ug
$757.00

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.