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Cdkl1 (NM_183294) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG213588
Cdkl1 (tGFP-tagged) - Mouse cyclin-dependent kinase-like 1 (CDC2-related kinase) (Cdkl1), (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $657.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cdkl1
Synonyms 4933411O17Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG213588 representing NM_183294
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAAAATATGAAAAAATTGGAAAGATTGGAGAAGGCTCCTATGGGGTAGTGTTCAAGTGCAGAAACA
GGGACACGGGTCAGATCGTGGCCATCAAGAGGTTTCTGGAAACCGAAGATGACCCTGTCATAAAGAAAAT
CGCCCTTCGAGAAATCCGCATGCTCAAGCAACTCAAGCACCCCAACCTGGTCAACCTCCTGGAAGTCTTC
CGGAGGAAGCGGAGGCTTCACCTGGTGTTCGAGTACTGCGACCACACGGTGCTTCACGAGCTGGATCGGT
ATCAGAGGGGGGTACCAGAGCCTCTCGTGAAGAACATAACTTGGCAGACACTGCAGGCTGTAAATTTCTG
CCATAAACATAACTGCATACACAGAGACGTGAAGCCGGAAAATATTCTCATCACCAAACAGTCAGCCATT
AAGCTCTGTGACTTTGGGTTCGCACGGCTTCTCACTGGACCAGGTGACTACTACACGGACTACGTGGCCA
CCCGGTGGTACCGCTCACCCGAGCTGCTAGTGGGAGACACGCAGTATGGTCCCCCTGTAGATGTCTGGGC
AATTGGCTGTGTGTTTGCTGAGTTACTGTCCGGAGTGCCTCTATGGCCAGGAAAATCTGACGTGGATCAG
CTCTACCTGATAAGGAAAACCCTGGGGGACCTCATTCCCAGGCACCAGCAAGTATTTAGCATGAATCAAT
ACTTCAGTGGGGTGAAAATTCCAGACCCTGAAGACATGGAAACACTTGAGTTGAAATTTCCAAACATCTC
CTACTCTGCACTGGGCTTCTTAAAGGGCTGCCTCCACATGGATCCTGCTGAGAGGCTGACATGCGAACAG
CTGTTGCAGCATCCATATTTTGACAGCATTCGAGAAGTTGGGGAGTTGACGAGACAACATGACAAACCAG
CGAGGAAGACTCTGAGGCAGAGTAGAAAGCACTTGACTGGGCTGCAGTACCTACCTCAGCTAACCAGCAG
CCGCATCCTTCCAGCTTTGGACAATAAGAAGTACCACTGTAGTACCAAGAGATTTAACTACCATTTTCCA
AATATT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG213588 representing NM_183294
Red=Cloning site Green=Tags(s)

MEKYEKIGKIGEGSYGVVFKCRNRDTGQIVAIKRFLETEDDPVIKKIALREIRMLKQLKHPNLVNLLEVF
RRKRRLHLVFEYCDHTVLHELDRYQRGVPEPLVKNITWQTLQAVNFCHKHNCIHRDVKPENILITKQSAI
KLCDFGFARLLTGPGDYYTDYVATRWYRSPELLVGDTQYGPPVDVWAIGCVFAELLSGVPLWPGKSDVDQ
LYLIRKTLGDLIPRHQQVFSMNQYFSGVKIPDPEDMETLELKFPNISYSALGFLKGCLHMDPAERLTCEQ
LLQHPYFDSIREVGELTRQHDKPARKTLRQSRKHLTGLQYLPQLTSSRILPALDNKKYHCSTKRFNYHFP
NI

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_183294
ORF Size 1056 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_183294.2, NP_899117.1
RefSeq Size 1689 bp
RefSeq ORF 1059 bp
Locus ID 71091
UniProt ID Q8CEQ0
Cytogenetics 12 C2
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"NM_183294" in other vectors (4)
SKU Description Size Price
MC211138 Cdkl1 (untagged) - Mouse cyclin-dependent kinase-like 1 (CDC2-related kinase) (Cdkl1), (10ug) 10 ug
$503.00
MR213588 Cdkl1 (Myc-DDK-tagged) - Mouse cyclin-dependent kinase-like 1 (CDC2-related kinase) (Cdkl1) 10 ug
$289.00 $457.00
MR213588L3 Lenti ORF clone of Cdkl1 (Myc-DDK-tagged) - Mouse cyclin-dependent kinase-like 1 (CDC2-related kinase) (Cdkl1) 10 ug
$757.00
MR213588L4 Lenti ORF clone of Cdkl1 (mGFP-tagged) - Mouse cyclin-dependent kinase-like 1 (CDC2-related kinase) (Cdkl1) 10 ug
$757.00

Plasmid Map for MG213588

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG213588
Circular map for MG213588

file-alt Citations

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