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Ces2e (NM_172759) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG212946
Ces2e (tGFP-tagged) - Mouse carboxylesterase 5 (Ces5) transcript variant 1, (10ug)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP tGFP DDK
AAV Particle:
DDK
  $721.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ces2e
Synonyms 9030624L02Rik; Ces5
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG212946 representing NM_172759
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCACTATACAAACTTCTTGGATGGCTGAATGCTGTGGCCTGTGGGGTCCTGCTTCTCGTTCTGCATG
TGCAAGGTCAGGACTCAGCCAGCCCCATCAGGAACACACACACAGGCCAGGTGAGAGGCAGCCTTGTCCA
CGTGAAGGACACTGACATCGCTGTTCATACCTTCCTGGGAATCCCCTTTGCCAAGCCTCCTGTAGGACCA
CTGCGCTTTGCACCTCCTGAGGCCCCTGAGCCATGGAGTGGGGTGAGGGATGGGACCTCACATCCAAACA
TGTGTCTACAAAATGACAATTTGATGGGTTCAGAGGATCTGAAGATGATGAACTTGATCCTGCCTCCCAT
CTCTATGTCTGAGGACTGCCTGTATCTCAACATCTATGTACCAGCCCATGCCCATGAGGGTTCTAACCTA
CCTGTGATGGTATGGATCCATGGTGGTGCACTCACTGTTGGCATGGCTTCCATGTATGATGGATCCATGC
TGGCAGCAACTGAGGATGTGGTGGTGGTCGCTATCCAGTACCGCCTGGGTGTTCTGGGATTCTTCAGCAC
TGGAGACCAGCATGCCAAAGGCAACTGGGGATACCTGGACCAAGTGGCTGCCCTTCGTTGGGTCCAGCAA
AACATTGTCCACTTTGGAGGCAACCCTGACCGAGTCACCATTTTTGGAGAGTCAGCAGGTGGCACAAGTG
TGTCTTCACATGTTGTGTCCCCCATGTCCCAGGGACTCTTCCATGGTGCCATCATGGAGAGTGGAGTAGC
TGTGCTCCCTGATCTTATCTCCAGCTCCTCTGAGATGGTTCACAGAATAGTGGCCAATCTATCTGGCTGT
GCAGCTGTGAACTCAGAGACCCTGATGTGCTGCCTAAGAGGCAAGAATGAAGCAGAGATGCTGGCTATTA
ATAAGGTCTTCAAAATCATCCCTGGTGTGGTGGATGGAGAGTTCCTACCCAAGCATCCTCAGGAGCTGAT
GGCCTCTAAGGATTTTCACCCTGTCCCCAGCATCATTGGTATCAACAATGATGAATATGGTTGGATTCTT
CCTACGATCATGGACCCAGCTCAGAAAATAGAGGAAATAACCAGAAAGACCCTGCCAGCTGTTCTAAAAA
GCACAGCCCTAAAAATGATGCTGCCTCCTGAGTGTGGTGACCTGCTAATGGAGGAGTACATGGGGGACAC
TGAGGACCCAGAGACTCTGCAAGCACAGTTCAGAGAGATGAAGGGGGACTTCATGTTCGTGATCCCTGCA
CTACAAGTAGCACATTTTCAACGTTCCCATGCTCCTGTCTACTTCTATGAGTTCCAACATCGACCCAGCT
TCTTCAAGGATTTCAGGCCACCCTATGTGAAGGCTGACCATGGTGATGAAATTTTCCTTGTCTTTGGCTA
CCAGTTCGGTAACATAAAACTTCCTTACACTGAGGAAGAGGAGCAACTAAGCAGAAGGATAATGAAGTAC
TGGGCCAACTTTGCACGGCACGGGAACCCCAACAGTGAGGGTCTACCCTACTGGCCTGTGATGGACCATG
ATGAGCAGTACCTGCAGCTGGACATCCAGCCTTCTGTGGGTCGAGCCCTGAAGGCCAGAAGGCTGCAGTT
CTGGACCAAGACTCTGCCCCAGAAGATCCAGGAGCTAAAGGGGTCTCAGGAGAGGCACAAAGAGTTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG212946 representing NM_172759
Red=Cloning site Green=Tags(s)

MPLYKLLGWLNAVACGVLLLVLHVQGQDSASPIRNTHTGQVRGSLVHVKDTDIAVHTFLGIPFAKPPVGP
LRFAPPEAPEPWSGVRDGTSHPNMCLQNDNLMGSEDLKMMNLILPPISMSEDCLYLNIYVPAHAHEGSNL
PVMVWIHGGALTVGMASMYDGSMLAATEDVVVVAIQYRLGVLGFFSTGDQHAKGNWGYLDQVAALRWVQQ
NIVHFGGNPDRVTIFGESAGGTSVSSHVVSPMSQGLFHGAIMESGVAVLPDLISSSSEMVHRIVANLSGC
AAVNSETLMCCLRGKNEAEMLAINKVFKIIPGVVDGEFLPKHPQELMASKDFHPVPSIIGINNDEYGWIL
PTIMDPAQKIEEITRKTLPAVLKSTALKMMLPPECGDLLMEEYMGDTEDPETLQAQFREMKGDFMFVIPA
LQVAHFQRSHAPVYFYEFQHRPSFFKDFRPPYVKADHGDEIFLVFGYQFGNIKLPYTEEEEQLSRRIMKY
WANFARHGNPNSEGLPYWPVMDHDEQYLQLDIQPSVGRALKARRLQFWTKTLPQKIQELKGSQERHKEL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172759
ORF Size 1677 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172759.3, NP_766347.1
RefSeq Size 2699 bp
RefSeq ORF 1680 bp
Locus ID 234673
UniProt ID Q8BK48
Cytogenetics 8 D3
Summary Carboxylesterase that catalyzes the hydrolysis of pyrethroids pesticides. Hydrolyzes trans-permethrin at a rate about 22-fold higher than cis-permethrin. Also hydrolyzes trans-cypermethrin.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_172759" in other vectors (5)
SKU Description Size Price
MC205461 Ces2e (untagged) - Mouse carboxylesterase 2E (Ces2e), transcript variant 1, (10ug) 10 ug
$521.00
MG208871 Ces2e (tGFP-tagged) - Mouse carboxylesterase 5 (Ces5) 10 ug
$721.00
MR212946 Ces2e (Myc-DDK-tagged) - Mouse carboxylesterase 2E (Ces2e), transcript variant 1 10 ug
$289.00 $521.00
MR212946L3 Lenti ORF clone of Ces2e (Myc-DDK-tagged) - Mouse carboxylesterase 2E (Ces2e), transcript variant 1 10 ug
$821.00
MR212946L4 Lenti ORF clone of Ces2e (mGFP-tagged) - Mouse carboxylesterase 2E (Ces2e), transcript variant 1 10 ug
$821.00

Plasmid Map for MG212946

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG212946
Circular map for MG212946

file-alt Citations

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