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Cacna2d2 (NM_020263) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG211721
Cacna2d2 (tGFP-tagged) - Mouse calcium channel, voltage-dependent, alpha 2/delta subunit 2 (Cacna2d2)
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ORF Plasmid:
tGFP tGFP DDK
  $1,206.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cacna2d2
Synonyms a2d2; Cacna2d; du; mKIAA0558; td; torpid
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG211721 representing NM_020263
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGTGCCGGCTCGGACCTGCGGCGCTTCTTGGCCCGGCCCGGTGCGGACCGCTCGCCCCTGGCCCG
GTCGCGGCCCCCGGCCCTGCCCTGACCCCCGGGGCCCAGCGTCCGGGCCCGCACGCCCGCTCTTGCTCTT
GCTGCCGCCTCTGTTGCTTTTACCTCTGCTCACCGCCCCCGGCGCCTCTGCCTACAGCTTCCCCCAGCAG
CACACGATGCAGCACTGGGCCCGGCGCCTGGAGCAGGAGATTGACGGTGTGATGCGGATTTTTGGAGGCG
TGCAGCAGCTCCGAGAGATCTACAAGGACAATCGGAACCTGTTTGAAGTGCAGGAGAATGAACCACAGAA
ATTGGTGGAGAAAGTGGCAGGGGACATCGAGAGCCTGCTGGACAGGAAGGTCCAGGCCCTGAAGAGACTG
GCTGACGCTGCAGAGAATTTCCAGAAAGCCCATCGCTGGCAGGACAACATCAAGGAGGAAGACATCATGT
ACTACGATGCCAAGGCTGACGCCGAGCTGGATGACCCTGAGAGTGAAGATATGGAGAGGGGATCCAAGAC
CAGCGCCTTAAGGCTGGACTTCATCGAGGACCCAAACTTCAAGAACAAAGTCAACTATTCATACACGGCT
GTGCAGATCCCCACAGACATCTACAAAGGCTCTACCGTCATCCTCAATGAGCTTAACTGGACAGAGGCCC
TGGAGAACGTCTTCATTGAGAACCGTAGGCAAGACCCTACACTGTTGTGGCAAGTCTTTGGCAGTGCCAC
GGGAGTCACTCGCTATTACCCAGCCACACCATGGCGAGCCCCCAAGAAGATTGACCTGTACGATGTCAGA
AGACGACCCTGGTATATACAGGGGGCCTCATCACCCAAGGACATGGTCATCATTGTGGATGTGAGTGGCA
GCGTGAGCGGCCTGACTCTGAAGCTGATGAAGACGTCCGTCTGTGAGATGCTAGACACGCTCTCTGATGA
TGACTATGTGAACGTGGCCTCATTCAACGAGAAGGCGCAGCCTGTGTCTTGCTTCACACACCTGGTGCAG
GCCAATGTGCGTAACAAGAAGGTGTTCAAGGAAGCTGTGCAGGGCATGGTGGCCAAGGGCACCACAGGCT
ACAAGGCCGGCTTTGAGTATGCCTTTGACCAGCTACAGAATTCCAACATCACGCGGGCTAACTGCAATAA
GATGATCATGATGTTCACGGATGGGGGTGAGGATCGCGTGCAGGATGTCTTCGAAAAGTACAATTGGCCC
AATCGGACGGTACGTGTGTTCACGTTCTCCGTAGGACAGCATAACTATGATGTCACACCCTTGCAGTGGA
TGGCCTGTACTAACAAAGGTTACTATTTTGAGATCCCTTCCATCGGAGCCATCCGCATCAACACACAGGA
ATACCTGGATGTGCTGGGTAGGCCCATGGTACTGGCAGGCAAGGACGCCAAGCAAGTGCAATGGACAAAC
GTGTATGAAGATGCACTGGGGCTGGGGTTGGTGGTAACAGGAACTCTCCCTGTTTTCAACCTGACACAGG
ATGGCCCTGGGGAAAAGAAGAACCAGTTAATCCTGGGTGTCATGGGCATCGATGTGGCCTTGAATGACAT
CAAAAGGCTGACTCCCAACTACACACTCGGCGCCAATGGCTATGTGTTCGCCATCGACCTGAACGGCTAC
GTGTTGCTACATCCCAATCTCAAGCCCCAGACTACCAACTTCCGGGAGCCTGTGACCTTGGACTTCCTGG
ACGCAGAGCTGGAAGATGAGAACAAGGAGGAGATCCGTCGCAGCATGATTGACGGCGACAAAGGCCACAA
GCAGATCAGAACCTTGGTCAAATCCCTGGATGAGAGGTACATAGACGAAGTGATTCGGAACTACACCTGG
GTGCCTATAAGGAGTACCAACTACAGCCTGGGGCTGGTGCTCCCACCCTACAGCACCTACTACCTCCAAG
CCAACCTCAGCGACCAGATCCTGCAGGTCAAGTTGCCAATCAGCAAACTGAAGGATTTTGAGTTCCTGCT
TCCCAGCAGCTTTGAGTCTGAAGGACACGTTTTCATTGCTCCGAGAGAGTATTGCAAGGACTTGAACGCC
TCGGACAACAACACCGAGTTCCTGAAGAACTTCATCGAGCTCATGGAGAAAGTGACTCCGGACTCCAAGC
AGTGTAATAACTTCCTTCTTCATAACTTGATTCTGGACACGGGCATTACACAGCAGTTAGTGGAACGTGT
GTGGCGGGACCAAGATCTCAACACGTACAGCCTGCTAGCCGTATTTGCTGCCACTGATGGTGGCATCACA
CGTGTCTTCCCGAACAAGGCAGCCGAAGACTGGACAGAAAACCCTGAACCCTTCAATGCCAGCTTCTACC
GTCGCAGCCTGGATAACCATGGTTATATCTTCAAGCCCCCACACCAGGACTCCCTGTTAAGACCACTGGA
GCTGGAGAATGACACAGTAGGTGTCCTCGTCAGCACAGCTGTGGAGCTCAGTCTAGGTCGTCGCACACTG
AGGCCAGCAGTGGTGGGTGTCAAACTGGACCTAGAGGCTTGGGCTGAAAAGTTCAAGGTGCTGGCCAGCA
ACCGTACCCATCAGGACCAACCTCAGAAGTGCGGCCCCAGCAGCCACTGTGAGATGGACTGCGAGGTTAA
CAACGAGGATCTACTCTGTGTCCTCATTGATGACGGAGGGTTCCTGGTGCTGTCAAACCAGAACCATCAG
TGGGACCAGGTTGGCAGATTCTTCAGTGAGGTGGATGCCAACCTGATGCTGGCACTGTACAATAACTCCT
TCTACACCCGAAAGGAATCCTATGACTATCAGGCAGCCTGTGCCCCTCAGCCTCCTGGGAACCTGGGTGC
TGCACCCCGGGGTGTCTTTGTGCCCACCATTGCAGATTTCCTTAACTTGGCCTGGTGGACCTCTGCTGCC
GCCTGGTCCTTATTCCAGCAGCTACTCTATGGTCTCATCTATCACAGCTGGTTCCAGGCAGACCCGGCAG
AAGCTGAGGGCAGCCCCGAGACGCGCGAGAGCAGCTGCGTCATGAAACAGACCCAGTACTACTTCGGCTC
GGTGAACGCATCCTATAATGCCATCATTGACTGCGGAAACTGCAGCAGGCTGTTCCATGCGCAGAGACTG
ACCAACACCAATCTCCTGTTCGTGGTGGCCGAGAAGCCGCTGTGCAGCCAGTGCGAGGCGGGCCGGCTGC
TGCAGAAGGAGACACACTCGGACGGCCCGGAGCAGTGTGAGCTGGTGCAGAGACCGAGATACCGAAGAGG
TCCGCACATCTGTTTTGACTACAATGCGACGGAAGATACCTCAGACTGTGGCCGCGGAGCCTCCTTCCCT
CCGTCGCTGGGCGTCTTGGTTTCCTTGCAGCTTTTGCTCCTCCTGGGCCTGCCACCTCGGCCGCAGCCTC
AAGTCCACTCCTTCGCTGCCTCTCGCCACCTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG211721 representing NM_020263
Red=Cloning site Green=Tags(s)

MAVPARTCGASWPGPVRTARPWPGRGPRPCPDPRGPASGPARPLLLLLPPLLLLPLLTAPGASAYSFPQQ
HTMQHWARRLEQEIDGVMRIFGGVQQLREIYKDNRNLFEVQENEPQKLVEKVAGDIESLLDRKVQALKRL
ADAAENFQKAHRWQDNIKEEDIMYYDAKADAELDDPESEDMERGSKTSALRLDFIEDPNFKNKVNYSYTA
VQIPTDIYKGSTVILNELNWTEALENVFIENRRQDPTLLWQVFGSATGVTRYYPATPWRAPKKIDLYDVR
RRPWYIQGASSPKDMVIIVDVSGSVSGLTLKLMKTSVCEMLDTLSDDDYVNVASFNEKAQPVSCFTHLVQ
ANVRNKKVFKEAVQGMVAKGTTGYKAGFEYAFDQLQNSNITRANCNKMIMMFTDGGEDRVQDVFEKYNWP
NRTVRVFTFSVGQHNYDVTPLQWMACTNKGYYFEIPSIGAIRINTQEYLDVLGRPMVLAGKDAKQVQWTN
VYEDALGLGLVVTGTLPVFNLTQDGPGEKKNQLILGVMGIDVALNDIKRLTPNYTLGANGYVFAIDLNGY
VLLHPNLKPQTTNFREPVTLDFLDAELEDENKEEIRRSMIDGDKGHKQIRTLVKSLDERYIDEVIRNYTW
VPIRSTNYSLGLVLPPYSTYYLQANLSDQILQVKLPISKLKDFEFLLPSSFESEGHVFIAPREYCKDLNA
SDNNTEFLKNFIELMEKVTPDSKQCNNFLLHNLILDTGITQQLVERVWRDQDLNTYSLLAVFAATDGGIT
RVFPNKAAEDWTENPEPFNASFYRRSLDNHGYIFKPPHQDSLLRPLELENDTVGVLVSTAVELSLGRRTL
RPAVVGVKLDLEAWAEKFKVLASNRTHQDQPQKCGPSSHCEMDCEVNNEDLLCVLIDDGGFLVLSNQNHQ
WDQVGRFFSEVDANLMLALYNNSFYTRKESYDYQAACAPQPPGNLGAAPRGVFVPTIADFLNLAWWTSAA
AWSLFQQLLYGLIYHSWFQADPAEAEGSPETRESSCVMKQTQYYFGSVNASYNAIIDCGNCSRLFHAQRL
TNTNLLFVVAEKPLCSQCEAGRLLQKETHSDGPEQCELVQRPRYRRGPHICFDYNATEDTSDCGRGASFP
PSLGVLVSLQLLLLLGLPPRPQPQVHSFAASRHL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_020263
ORF Size 3462 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_020263.2
RefSeq Size 5498 bp
RefSeq ORF 3465 bp
Locus ID 56808
UniProt ID Q6PHS9
Cytogenetics 9 58.02 cM
Summary The alpha-2/delta subunit of voltage-dependent calcium channels regulates calcium current density and activation/inactivation kinetics of the calcium channel. Acts as a regulatory subunit for P/Q-type calcium channel (CACNA1A), N-type (CACNA1B), L-type (CACNA1C OR CACNA1D) and possibly T-type (CACNA1G).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_020263" in other vectors (5)
SKU Description Size Price
MC223767 Cacna2d2 (untagged) - Mouse calcium channel, voltage-dependent, alpha 2/delta subunit 2 (Cacna2d2), transcript variant 2, (10ug) 10 ug
$1,106.00
MG222801 Cacna2d2 (tGFP-tagged) - Mouse calcium channel voltage-dependent alpha 2/delta subunit 2 (Cacna2d2) transcript variant 2, (10ug) 10 ug
$1,708.00
MR222801 Cacna2d2 (Myc-DDK-tagged) - Mouse calcium channel, voltage-dependent, alpha 2/delta subunit 2 (Cacna2d2), transcript variant 2 10 ug
$1,508.00
MR222801L3 Lenti ORF clone of Cacna2d2 (Myc-DDK-tagged) - Mouse calcium channel, voltage-dependent, alpha 2/delta subunit 2 (Cacna2d2), transcript variant 2 10 ug
$1,808.00
MR222801L4 Lenti ORF clone of Cacna2d2 (mGFP-tagged) - Mouse calcium channel, voltage-dependent, alpha 2/delta subunit 2 (Cacna2d2), transcript variant 2 10 ug
$1,808.00

Plasmid Map for MG211721

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG211721
Circular map for MG211721

file-alt Citations

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