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Adar (BC042505) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG211714
Adar (tGFP-tagged) - Mouse adenosine deaminase, RNA-specific (cDNA clone MGC:28306 IMAGE:4012994)
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$789.00 $1,204.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Adar
Synonyms Adar1, mZaADAR
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC042505
ORF Size 3458 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC042505, AAH42505
RefSeq Size 3764 bp
RefSeq ORF 3458 bp
Locus ID 56417
Cytogenetics 3 F1
Summary Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites (hyper-editing) or at specific sites (site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein (BLCAP), neurotransmitter receptors for glutamate (GRIA2) and serotonin (HTR2C) and GABA receptor (GABRA3). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alters their functional activities. Exhibits low-level editing at the GRIA2 Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Does not affect polyomavirus replication but provides protection against virus-induced cytopathic effects. Essential for embryonic development and cell survival and plays a critical role in the maintenance of hematopoietic stem cells.UniProtKB/Swiss-Prot Function
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Documents
Resources

Plasmid Map for MG211714

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file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG211714
Circular map for MG211714

file-alt Citations

Phenotypic Variation in Aicardi-Goutières Syndrome Explained by Cell-Specific IFN-Stimulated Gene Response and Cytokine Release
Cuadrado, Michailidou, van Bodegraven et al
J. Immunol. (2015)
Cited by 49
AI Summary: In this study, OriGene's rabbit anti-RNASEH2A antibody (TA306706) was used to detect RNASEH2A protein levels by immunoblotting, enabling the authors to assess the effects of gene silencing on protein expression in their investigation of Aicardi-Goutières syndrome.
Snippet: Blots were blocked in SuperMix (0.606% Tris, 0.9% NaCl, 0.25% gelatin [pH 7.4]) and incubated overnight with primary Abs: rabbit anti-TREX1 (1:300, Rab34, a gift of Dr. Tomas Lindahl), rabbit anti-RNASEH2A (1:2000, Origene, Rockville, MD, TA306706), mouse anti ... Cite SaveSaving
WB
Showing 1-1 of 1 papers.
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