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Ppip5k2 (NM_173760) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG211662
Ppip5k2 (tGFP-tagged) - Mouse histidine acid phosphatase domain containing 1 (Hisppd1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
  $1,183.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ppip5k2
Synonyms AW555814; Cfap160; D330021B20; Hisppd1; mKIAA0433; Vip2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG211662 representing NM_173760
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGAACCCCCCAGATTCTTTGTTGGGCCCGAAGATGCGGAAATCAATCCTGGAAACTATCGTCGCT
TCTTCCACCATGCTGAAGAGGAGGAGGAGGAGGAGGATGAGTCTCCACCAGAAAGGCAGATTGTGGTTGG
AATATGTTCCATGGCAAAGAAATCCAAATCCAAACCCATGAAGGAAATTCTGGAACGGATCTCCTTGTTT
AAGTACATCACAGTAGTGGTTTTTGAAGAGGAAATTATTTTGAATGAACCCGTGGAAAACTGGCCTTTAT
GTGATTGTCTTATTTCTTTCCATTCTAAAGGATTTCCACTGGACAAAGCAGTTGCCTATGCAAAACTCAG
GAATCCATTTGTAATCAATGACTTGAATATGCAGTATCTCATACAAGATAGAAGAGACGTGTATAGCATT
CTTCAAGCTGAAGGCATCCTACTTCCTCGGTATGCAATCCTGAACCGTGATCCAAATAACCCGAAAGAAT
GCAACCTGATTGAAGGAGAAGATCATGTAGAAGTGAATGGGGAAGTTTTCCAAAAGCCCTTTGTGGAGAA
GCCGGTCAGTGCAGAGGACCACAATGTTTACATTTATTATCCAACATCCGCCGGAGGTGGGAGTCAGAGA
CTTTTCCGGAAGATTGGCAGTAGAAGTAGTGTGTATTCCCCAGAGAGCAACGTTCGAAAAACGGGGTCAT
ATATATATGAAGAGTTTATGCCTACAGATGGTACTGATGTTAAGGTTTATACCGTGGGTCCAGACTATGC
ACATGCTGAAGCTCGAAAATCTCCAGCTCTCGATGGCAAGGTAGAGCGAGACAGCGAAGGGAAGGAAGTA
AGATACCCCGTCATCCTCAATGCACGGGAGAAGCTGATTGCGTGGAAAGTCTGCCTTGCTTTTAAGCAAA
CGGTTTGTGGCTTTGATTTGTTACGTGCCAATGGACAATCCTATGTATGCGATGTCAATGGCTTCAGTTT
TGTGAAAAATTCCATGAAGTACTATGATGACTGTGCAAAAATTCTTGGCAATATCGTAATGCGGGAGCTT
GCACCACAGTTTCATATCCCGTGGTCAATACCATTAGAAGCTGAAGATATTCCAATTGTACCAACTACCT
CTGGAACTATGATGGAACTTCGATGTGTCATCGCTGTGATACGACATGGGGATCGAACACCGAAACAGAA
AATGAAAATGGAAGTGAGGCATCAGAAGTTTTTTGACCTTTTTGAAAAGTGTGATGGATATAAATCAGGG
AAGTTAAAGCTCAAGAAGCCAAAACAATTACAGGAAGTCCTGGATATTGCAAGGCAGCTTCTTATGGAGC
TGGGGCAAAATAATGACTCTGAAATTGAAGAAAACAAGTCAAAACTTGAACAACTTAAGACTGTGTTAGA
GATGTATGGTCATTTTTCAGGAATAAACCGTAAGGTCCAATTAACCTATCTTCCTCATGGCTGTCCGAAA
ACATCCAGTGAGGAGGAAGATAACCGAAGAGAGGAGCCGTCCTTACTTTTGGTTCTAAAATGGGGAGGAG
AACTGACCCCTGCGGGCAGGGTTCAGGCTGAAGAGCTGGGAAGAGCTTTTCGGTGTATGTACCCTGGAGG
TCAAGGAGATTATGCAGGATTTCCTGGTTGTGGTTTACTCCGACTACATAGCACCTACAGGCATGACCTC
AAAATATATGCCTCTGATGAAGGGCGGGTCCAGATGACGGCGGCTGCTTTCGCAAAGGGTCTCTTGGCTC
TAGAAGGAGAGCTTACCCCCATTCTTGTTCAGATGGTGAAAAGTGCAAACATGAACGGCCTTTTGGATAG
TGATAGTGACTCTTTGAGTAGCTGTCAGCAGCGTGTGAAAGCGAGGCTTCATGAAATACTTCAGAAAGAC
AGGGATTTCACAGCTGAGGACTACGAGAAGCTTACTCCATCTGGAAGCATTTCTGTTATCAAATCAATGC
ATTTGATTAAAAATCCAGTGAAAACCTGTGACAAAGTGTATTCCTTGATTCAAAGTTTGACTTCCCAAAT
TAGATATCGGATGGAAGATCCCAAATCAGCTGATATTCAGCTTTACCACAGTGAGACCCTGGAACTTATG
CTGCGTAGATGGTCCAAGTTGGAGAAGGATTTTAAAACAAAGAATGGAAGATATGATATTAGCAAGATCC
CTGACATATATGACTGCATCAAATATGACGTTCAGCATAACGGTTCCTTGAAGTTAGAAAACACAATGGA
ACTATATAGGCTTTCAAAGGCATTAGCAGATATTGTTATCCCTCAGGAATATGGTATAACTAAAGCTGAA
AAACTGGAGATTGCCAAAGGCTACTGTACTCCACTAGTTAGAAAAATCCGATCAGATCTTCAGAGGACAC
AAGATGATGATACTGTCAATAAACTCCATCCTGTGTATTCTAGAGGTGTTCTGTCTCCTGAGCGGCACGT
CCGCACCAGATTATATTTTACCAGTGAGAGTCATGTGCATTCTTTACTATCCATTCTTCGGTATGGCGCC
TTATGCGATGACTCAAAGGATGAGCAGTGGAAACGAGCTATGGATTATCTAAACGTCGTCAATGAGCTCA
ACTACATGACGCAGATTGTTATCATGCTCTATGAGGATCCCAACAAGGACCTTTCCTCAGAGGAACGCTT
TCATGTTGAGTTACATTTTAGTCCTGGAGCCAAAGGCTGTGAAGAAGATAAGAATTTGCCATCTGGCTAT
GGGTATAGGCCAGCATCCAGGGAGAATGAAGGCAGAAGGTCTCTTAAAACTGACGATGATGAGCCTCACA
CTTCTAAACGAGATGAAGTTGACCGGGCCGTGATGTTGTTTAAACCATTGGTGTCTGAGCCAATTCATAT
ACACAGGAAATCACCGCTTCCGAGGTCCAGGAAGATTACTGCAAATGAGGTTGTGTCTGAGAATGCCAAC
TATCTCCGAACACCAAGAAATCTTGTGGAACAGAAGCAGAACCCTACAGTAGGCTTCGAGCTGTATTCCA
TGGTACCATCCATCTGTCCTCTAGAAACACTTCACAATGCCCTGTTTTTAAAGCAAGTGGATGACTTTCT
GGCTTCCATTGCTTCTCCATCAACGGAGGTTCTGCGCAAGGTCCCCGAAATGTCCTCTATGGCCACACGT
TCCAGTCCAGGAATGAGAAGAAAAATTTCCTTAAATACGTATACACCTACAAAGATCCTCCCAACACCAC
CTGCTGCTTTAAAGAGCAGCAAAGCAAGCAGCAAAGCAGCAGCCGGCGGACCATCTCAGGCAATGGCTCC
CCATACCTCATCTCGGAAGAAGAGCATAAATAGCAAAACCGAGGGGCATGAACCCAAGAAAAGCACTGGG
AAGAAAAGA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG211662 representing NM_173760
Red=Cloning site Green=Tags(s)

MSEPPRFFVGPEDAEINPGNYRRFFHHAEEEEEEEDESPPERQIVVGICSMAKKSKSKPMKEILERISLF
KYITVVVFEEEIILNEPVENWPLCDCLISFHSKGFPLDKAVAYAKLRNPFVINDLNMQYLIQDRRDVYSI
LQAEGILLPRYAILNRDPNNPKECNLIEGEDHVEVNGEVFQKPFVEKPVSAEDHNVYIYYPTSAGGGSQR
LFRKIGSRSSVYSPESNVRKTGSYIYEEFMPTDGTDVKVYTVGPDYAHAEARKSPALDGKVERDSEGKEV
RYPVILNAREKLIAWKVCLAFKQTVCGFDLLRANGQSYVCDVNGFSFVKNSMKYYDDCAKILGNIVMREL
APQFHIPWSIPLEAEDIPIVPTTSGTMMELRCVIAVIRHGDRTPKQKMKMEVRHQKFFDLFEKCDGYKSG
KLKLKKPKQLQEVLDIARQLLMELGQNNDSEIEENKSKLEQLKTVLEMYGHFSGINRKVQLTYLPHGCPK
TSSEEEDNRREEPSLLLVLKWGGELTPAGRVQAEELGRAFRCMYPGGQGDYAGFPGCGLLRLHSTYRHDL
KIYASDEGRVQMTAAAFAKGLLALEGELTPILVQMVKSANMNGLLDSDSDSLSSCQQRVKARLHEILQKD
RDFTAEDYEKLTPSGSISVIKSMHLIKNPVKTCDKVYSLIQSLTSQIRYRMEDPKSADIQLYHSETLELM
LRRWSKLEKDFKTKNGRYDISKIPDIYDCIKYDVQHNGSLKLENTMELYRLSKALADIVIPQEYGITKAE
KLEIAKGYCTPLVRKIRSDLQRTQDDDTVNKLHPVYSRGVLSPERHVRTRLYFTSESHVHSLLSILRYGA
LCDDSKDEQWKRAMDYLNVVNELNYMTQIVIMLYEDPNKDLSSEERFHVELHFSPGAKGCEEDKNLPSGY
GYRPASRENEGRRSLKTDDDEPHTSKRDEVDRAVMLFKPLVSEPIHIHRKSPLPRSRKITANEVVSENAN
YLRTPRNLVEQKQNPTVGFELYSMVPSICPLETLHNALFLKQVDDFLASIASPSTEVLRKVPEMSSMATR
SSPGMRRKISLNTYTPTKILPTPPAALKSSKASSKAAAGGPSQAMAPHTSSRKKSINSKTEGHEPKKSTG
KKR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_173760
ORF Size 3369 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_173760.4
RefSeq Size 5565 bp
RefSeq ORF 3390 bp
Locus ID 227399
UniProt ID Q6ZQB6
Cytogenetics 1 D
Summary Bifunctional inositol kinase that acts in concert with the IP6K kinases IP6K1, IP6K2 and IP6K3 to synthesize the diphosphate group-containing inositol pyrophosphates diphosphoinositol pentakisphosphate, PP-InsP5, and bis-diphosphoinositol tetrakisphosphate, (PP)2-InsP4. PP-InsP5 and (PP)2-InsP4, also respectively called InsP7 and InsP8, regulate a variety of cellular processes, including apoptosis, vesicle trafficking, cytoskeletal dynamics, exocytosis, insulin signaling and neutrophil activation. Phosphorylates inositol hexakisphosphate (InsP6) at positions 1 or 3 to produce PP-InsP5 which is in turn phosphorylated by IP6Ks to produce (PP)2-InsP4. Alternatively, phosphorylates at position 1 or 3 PP-InsP5, produced by IP6Ks from InsP6, to produce (PP)2-InsP4.UniProtKB/Swiss-Prot Function
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Resources
"NM_173760" in other vectors (4)
SKU Description Size Price
MC204729 Ppip5k2 (untagged) - Mouse diphosphoinositol pentakisphosphate kinase 2 (Ppip5k2), (10ug) 10 ug
$978.00
MR211662 Ppip5k2 (Myc-DDK-tagged) - Mouse diphosphoinositol pentakisphosphate kinase 2 (Ppip5k2) 10 ug
$589.00 $983.00
MR211662L3 Lenti ORF clone of Ppip5k2 (Myc-DDK-tagged) - Mouse diphosphoinositol pentakisphosphate kinase 2 (Ppip5k2) 10 ug
$1,283.00
MR211662L4 Lenti ORF clone of Ppip5k2 (mGFP-tagged) - Mouse diphosphoinositol pentakisphosphate kinase 2 (Ppip5k2) 10 ug
$1,283.00

Plasmid Map for MG211662

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG211662
Circular map for MG211662

file-alt Citations

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