Ifih1 (BC080200) Mouse Tagged ORF Clone
- TrueORF®
SKU
MG211363
Ifih1 (tGFP-tagged) - Mouse interferon induced with helicase C domain 1 (cDNA clone MGC:90959 IMAGE:3597815)
$1,540.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Ifih1 |
| Synonyms | Helicard, Hlcd, MDA5, MDA-5 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | BC080200 |
| ORF Size | 2928 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | BC080200, AAH80200 |
| RefSeq Size | 3635 bp |
| RefSeq ORF | 2930 bp |
| Locus ID | 71586 |
| Cytogenetics | 2 C1.3 |
| Summary | Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include mRNA lacking 2'-O-methylation at their 5' cap and long-dsRNA (>1 kb in length). Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Responsible for detecting the Picornaviridae family members such as encephalomyocarditis virus (EMCV), mengo encephalomyocarditis virus (ENMG), and theiler's murine encephalomyelitis virus (TMEV). Can also detect other viruses such as dengue virus (DENV), west Nile virus (WNV), and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome, such as vaccinia virus. Plays an important role in amplifying innate immune signaling through recognition of RNA metabolites that are produced during virus infection by ribonuclease L (RNase L). May play an important role in enhancing natural killer cell function and may be involved in growth inhibition and apoptosis in several tumor cell lines.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Citations
Aberrant activation of the type I interferon system may contribute to the pathogenesis of anti-melanoma differentiation-associated gene 5 dermatomyositis
Zhang, Zhao, Xie et al
Br J Dermatol (2019) 180 (5), 1090-1098 DOI: 10.1111/bjd.16917
Zhang, Zhao, Xie et al
Br J Dermatol (2019) 180 (5), 1090-1098 DOI: 10.1111/bjd.16917
Cited by 85
AI Summary: In this study, OriGene products—specifically the STAT1, ISG15, and MxA antibodies—were used for immunohistochemical analysis of skin lesions, helping to demonstrate increased expression of interferon-stimulated proteins in anti-MDA5 dermatomyositis patients.
The Zinc-Finger Protein ZCCHC3 Binds RNA and Facilitates Viral RNA Sensing and Activation of the RIG-I-like Receptors
Lian, Zang, Wei et al
Immunity (2018) 49 (3), 438-448.e5 DOI: 10.1016/j.immuni.2018.08.014
Lian, Zang, Wei et al
Immunity (2018) 49 (3), 438-448.e5 DOI: 10.1016/j.immuni.2018.08.014
Cited by 142
AI Summary: In this study, OriGene products including the human ZCCHC3 expression plasmid (OriGene Cat# RC206642) and ZCCHC3-specific shRNA (OriGene Cat# TG316618) were used to manipulate ZCCHC3 levels in cells, enabling the authors to investigate its role in regulating RIG-I-like receptor-mediated antiviral responses.
Neutrophil extracellular traps may contribute to interstitial lung disease associated with anti-MDA5 autoantibody positive dermatomyositis
Peng, Zhang, Zhao et al
Clin. Rheumatol. (2017)
Peng, Zhang, Zhao et al
Clin. Rheumatol. (2017)
AI Summary: OriGene’s purified recombinant MDA5 protein was used in ELISA assays to detect anti-MDA5 autoantibody levels, enabling the study to assess their association with interstitial lung disease in dermatomyositis patients.
Snippet: … Detection of anti-MDA5 autoantibody and KL-6 Anti-MDA5 autoAb titers were determined by ELISA using purified recombinant protein of MDA5 (OriGene, Maryland, USA) as described previously [6, 17]. ...;... … Detection of anti-MDA5 autoantibody and KL-6 Anti-MDA5 autoAb titers were determined by ELISA using purified recombinant protein of MDA5 (OriGene, Maryland, USA) as described previously [6, 17]. ... …
Anti-MDA5 antibody is associated with A/SIP and decreased T cells in peripheral blood and predicts poor prognosis of ILD in Chinese patients with dermatomyositis
Chen, Wang, Shu et al
Rheumatol Int (2012) 32 (12), 3909-15 DOI: 10.1007/s00296-011-2323-y
Chen, Wang, Shu et al
Rheumatol Int (2012) 32 (12), 3909-15 DOI: 10.1007/s00296-011-2323-y
Cited by 115
AI Summary: In this study, OriGene's anti-MDA5 antibody (Cat# TA322322) was used to detect serum anti-MDA5 levels by ELISA, enabling the identification of its association with disease severity and prognosis in Chinese dermatomyositis patients.
The mucocutaneous and systemic phenotype of dermatomyositis patients with antibodies to MDA5 (CADM-140): a retrospective study
Fiorentino, Chung, Zwerner et al
J. Am. Acad. Dermatol. (2011) 65 (1), 25-34 DOI: 10.1016/j.jaad.2010.09.016
Fiorentino, Chung, Zwerner et al
J. Am. Acad. Dermatol. (2011) 65 (1), 25-34 DOI: 10.1016/j.jaad.2010.09.016
Cited by 556
AI Summary: OriGene’s full-length MDA5 cDNA was used in this study to produce MDA5 protein for in vitro assays, enabling the identification of anti-MDA5 antibodies in dermatomyositis patient plasma.
Snippet: in vitro transcription/translation. Full-length complementary DNAs were purchased (MDA5, Origene, Rockville, MD), cloned in our laboratory (Ro60), and the Mi-2 complementary DNA has been previously described. 29 [ 35 S
Showing 1-5 of 5 papers.
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