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Map4k5 (NM_024275) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG210941
Map4k5 (tGFP-tagged) - Mouse mitogen-activated protein kinase kinase kinase kinase 5 (Map4k5), transcript variant 2
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ORF Plasmid:
tGFP DDK
  $976.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Map4k5
Synonyms KHS, GCKR, MAPKKKK5, 4432415E19Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG210941 representing NM_024275
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGCCCCGCTGAGGCCTGCCGCCGACATCCTGAGGCGGAACCCGCAGCACGACTACGAGCTGGTGC
AGAGGGTCGGCAGCGGCACCTACGGGGACGTGTACAAGGCCAGAAATGTACATACAGGAGAATTAGCTGC
AGTAAAGATCATCAAGTTGGAGCCTGGTGATGATTTTTCTTTGATTCAGCAAGAGATATTTATGGTTAAA
GAATGTAAGCATTGCAACATCGTAGCCTACTTTGGGAGCTATCTCAGTAGGGAGAAGCTGTGGATTTGCA
TGGAGTACTGCGGTGGCGGATCACTTCAAGATATCTATCACGTGACTGGGCCATTATCTGAAATGCAAAT
AGCCTACGTCTGCAGAGAGACCTTACAGGGTCTTGCCTACTTGCATACTAAAGGCAAAATGCATAGAGAT
ATCAAAGGTGCAAATATCTTATTAACAGACCATGGTGATGTAAAATTAGCTGATTTTGGTGTGGCTGCAA
AAATTACAGCTACCATTGCAAAGAGGAAGTCCTTCATCGGCACCCCTTACTGGATGGCTCCAGAAGTTGC
AGCAGTGGAAAAGAATGGTGGCTACAACCAACTCTGTGATATCTGGGCCGTCGGAATAACAGCAATCGAG
CTGGGAGAACTGCAGCCGCCTATGTTTGACCTGCATCCAATGAGGGCTCTCTTCTTAATGTCAAAAAGTA
ATTTCCAGCCTCCAAAGCTAAAGGACAAAACTAAATGGTCATCGACATTCCATAATTTTGTCAAAATAGC
ACTAACTAAAAACCCCAAGAAAAGACCAACTGCTGAAAGGCTTCTGACTCACACTTTTGTTGGGCAGCCA
GGCCTGTCTCGGGCGCTCGCGGTTGAGCTGTTGGACAAGGTGAGCAATCCGGACAACCATGCTCCCTACA
GTGAGGGCGATGAGGACGACTTGGAGCCCCACGCAATCATTCGTCATACCATTAGATCAACAAACAGGAA
TTCCAGAGCGGAGCGGACAGCTTCAGAAATAAACTTTGACAAATTACAATTTGAACCTCCGCTGAGAAAA
GAAACAGAAGCCCGGGATGAAATGGGATTATCATCAGAGCCAAACTTTATATTACATTGGAATCCTTTTG
TGGATGGAGCAAATACAGGCAGATCAACCTCTAAGCGTGCAATACCACCTCCGCTACCTCCTAAGCCGAG
GGTCAACACTTACCCTGAAGACAGCCTCCCAGATGAAGAAAAATCATCAACCATCAAACGTTGCCCTGAT
CTGGAGGCGAGAGCACCTCAGGTCCTCAGAAGACAGAGTAGCCCAAGTTGTGTTCCTGTGGTGGAGACAT
CCTCCTCTATAGGAAATGGTGATGGTATTTCAAAACTGATCAGTGAAAATACAGAGGGATCCGCACAAGC
TCCGCAGTTACCTCGGAAAAAGGACAAGCGGGACTTCCCCAAACCAACCATCAATGGCCTTCCACCCACC
CCAAAAGTACTGATGGGAGCATGTTTTTCAAAAGTATTTGATGGCTGCCCTTTGAAAATTAATTGTGCAA
CATCCTGGATACACCCTGATACAAAAGATCAGTATATTATTTTTGGAACTGAAGATGGTATTTACACATT
GAATCTCAATGAGCTACATGAAGCAACTATGGAACAGTTATTTCCACGGAAATGTACGTGGCTCTATGTC
ATCAATAATACACTGATGTCATTATCAGAAGGAAAAACCTTTCAGCTCTACTCTCACAACCTTATTGCTT
TGTTTGAACAAGCCAAAAAACCAGGGTTAGCCGCCCACATCCAAACTCACAGGTTTCCTGACCGCATTCT
GCCAAGAAAATTTGCCTTAACAACTAAGATTCCCGATACAAAAGGCTGCCACAAATGCTGCATAGTTAGA
AACCCTTACACGGGACATAAGTACCTCTGCGGAGCTTTGCAGTCTGGCATCGTTCTGCTTCAGTGGTATG
AGCCGATGCAGAAATTCATGCTGATAAAGCATTTCGATTTTCCTTTACCAAGTCCACTGAATGTTTTTGA
AATGCTGGTGATCCCAGAGCAGGAGTACCCCATGGTTTGTGTGGCTATCAGCAAAGGCTCGGACTCCAGC
CAAGTAGTTCAGTTTGAGACAATCAACTTGAATTCTGCATCTTCCTGGTTTACAGAAATTGGTGCAGGCA
GCCAACAGCTGGATTCCATTCATGTGACGCAGTTGGAGAGAGATACTGTTTTGGTGTGTTTAGACAAATT
TGTAAAAATTGTAAATCTACAAGGAAAGCTGAAGTCCAGTAAGAAGCTGGCATCTGAGCTCAGTTTTGAC
TTCCGCATTGAGTCTGTGGTGTGCCTTCAAGACAGCGTGTTGGCTTTCTGGAAACACGGGATGCAGGGTA
AAAGCTTCAAATCAGATGAGGTTACCCAGGAAATTTCAGATGAGACCAGGGTTTTCCGCCTGCTGGGATC
AGACAGGGTGGTGGTTTTGGAAAGCCGGCCAACAGAAAACCCTGCAGCACACAGCAATCTCTACATCCTG
GCTGGACATGAAAACAGTTAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG210941 representing NM_024275
Red=Cloning site Green=Tags(s)

MEAPLRPAADILRRNPQHDYELVQRVGSGTYGDVYKARNVHTGELAAVKIIKLEPGDDFSLIQQEIFMVK
ECKHCNIVAYFGSYLSREKLWICMEYCGGGSLQDIYHVTGPLSEMQIAYVCRETLQGLAYLHTKGKMHRD
IKGANILLTDHGDVKLADFGVAAKITATIAKRKSFIGTPYWMAPEVAAVEKNGGYNQLCDIWAVGITAIE
LGELQPPMFDLHPMRALFLMSKSNFQPPKLKDKTKWSSTFHNFVKIALTKNPKKRPTAERLLTHTFVGQP
GLSRALAVELLDKVSNPDNHAPYSEGDEDDLEPHAIIRHTIRSTNRNSRAERTASEINFDKLQFEPPLRK
ETEARDEMGLSSEPNFILHWNPFVDGANTGRSTSKRAIPPPLPPKPRVNTYPEDSLPDEEKSSTIKRCPD
LEARAPQVLRRQSSPSCVPVVETSSSIGNGDGISKLISENTEGSAQAPQLPRKKDKRDFPKPTINGLPPT
PKVLMGACFSKVFDGCPLKINCATSWIHPDTKDQYIIFGTEDGIYTLNLNELHEATMEQLFPRKCTWLYV
INNTLMSLSEGKTFQLYSHNLIALFEQAKKPGLAAHIQTHRFPDRILPRKFALTTKIPDTKGCHKCCIVR
NPYTGHKYLCGALQSGIVLLQWYEPMQKFMLIKHFDFPLPSPLNVFEMLVIPEQEYPMVCVAISKGSDSS
QVVQFETINLNSASSWFTEIGAGSQQLDSIHVTQLERDTVLVCLDKFVKIVNLQGKLKSSKKLASELSFD
FRIESVVCLQDSVLAFWKHGMQGKSFKSDEVTQEISDETRVFRLLGSDRVVVLESRPTENPAAHSNLYIL
AGHENSY

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024275
ORF Size 2541 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024275.2, NP_077237.2
RefSeq Size 4490 bp
RefSeq ORF 2543 bp
Locus ID 399510
Cytogenetics 12 C2
Summary May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_024275" in other vectors (4)
SKU Description Size Price
MC204859 Map4k5 (untagged) - Mouse mitogen-activated protein kinase kinase kinase kinase 5 (Map4k5), transcript variant 2, (10ug) 10 ug
$776.00
MR210941 Map4k5 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase kinase kinase 5 (Map4k5), transcript variant 2 10 ug
$589.00 $776.00
MR210941L3 Lenti ORF clone of Map4k5 (Myc-DDK-tagged) - Mouse mitogen-activated protein kinase kinase kinase kinase 5 (Map4k5), transcript variant 2 10 ug
$1,076.00
MR210941L4 Lenti ORF clone of Map4k5 (mGFP-tagged) - Mouse mitogen-activated protein kinase kinase kinase kinase 5 (Map4k5), transcript variant 2 10 ug
$1,076.00

Plasmid Map for MG210941

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG210941
Circular map for MG210941

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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