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Cpt1a (NM_013495) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG210568
Cpt1a (tGFP-tagged) - Mouse carnitine palmitoyltransferase 1a, liver (Cpt1a)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
$789.00 $908.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cpt1a
Synonyms C730027G07; Cpt1; CPTI
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG210568 representing NM_013495.
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGCAGAGGCTCACCAAGCTGTGGCCTTCCAGTTCACAGTCACCCCTGATGGCATCGATCTCCGCCTG
AGCCATGAAGCCCTCAAACAGATCTGCCTGTCAGGGCTGCACTCCTGGAAGAAGAAGTTCATCCGATTC
AAGAATGGCATCATCACTGGTGTGTTCCCCGCGAGTCCCTCCAGCTGGCTTATCGTGGTGGTGGGTGTG
ATATCATCCATGCATACCAAAGTGGACCCCTCCCTGGGCATGATTGCAAAGATCAATCGGACCCTAGAC
ACCACTGGCCGCATGTCAAGCCAGACGAAGAACATCGTGAGTGGCGTCCTCTTTGGCACAGGGCTCTGG
GTGGCGATCATCATGACTATGCGCTACTCGCTGAAGGTGCTGCTCTCCTACCATGGCTGGATGTTTGCA
GAGCACGGCAAAATGAGCCGCAGCACCAGAATCTGGATGGCTATGGTCAAGGTCTTCTCGGGTCGAAAG
CCCATGTTGTACAGCTTCCAGACGTCTCTGCCGCGCCTGCCTGTCCCAGCTGTCAAAGATACCGTGAGC
AGGTACCTGGAGTCTGTGAGGCCACTGATGAAGGAGGGAGACTTCCAACGCATGACAGCACTGGCCCAG
GATTTTGCTGTCAACCTTGGACCCAAATTGCAGTGGTATTTGAAGCTAAAATCCTGGTGGGCCACAAAT
TATGTGAGTGACTGGTGGGAGGAATACATCTACCTGCGGGGCCGAGGGCCGATCATGGTTAACAGCAAC
TACTACGCCATGGAGATGCTCTACATCACCCCAACCCATATTCAGGCAGCGAGAGCTGGCAACACCATC
CACGCCATACTGCTGTATCGTCGCACGGTAGACCGTGAGGAACTCAAACCTATTCGTCTTCTGGGATCT
ACAATTCCCCTCTGCTCTGCTCAGTGGGAGCGACTCTTCAATACTTCCCGCATCCCTGGGGAGGAGACA
GACACCATCCAACACGTCAAGGACAGCAGGCACATTGTCGTGTACCACAGAGGCCGTTACTTCAAGGTC
TGGCTCTACCATGACGGGAGGCTGCTGAGGCCCCGTGAGCTGGAGCAGCAGATGCAGCAGATCCTGGAT
GACACCTCAGAGCCGCAGCCCGGGGAAGCCAAGCTTGCCGCCCTCACTGCCGCAGACAGAGTGCCCTGG
GCGAAGTGTCGGCAGACCTATTTTGCACGAGGAAAAAATAAGCAATCTCTGGATGCGGTAGAAAAGGCA
GCATTCTTCGTGACGTTGGACGAATCGGAACAGGGATATAGAGAGGAGGACCCTGAGGCATCTATTGAC
AGCTATGCCAAATCTCTGCTGCATGGTAGATGTTTCGACAGGTGGTTTGACAAGTCCATCACCTTTGTT
GTCTTCAAAAACAGCAAGATAGGCATAAACGCAGAGCATTCCTGGGCGGACGCGCCCATCGTGGGCCAT
CTGTGGGAGTATGTCATGGCCACCGACGTCTTCCAGCTGGGCTACTCAGAGGATGGACACTGTAAAGGA
GACAAGAACCCCAACATCCCCAAACCCACCAGGCTACAGTGGGACATTCCAGGAGAATGCCAGGAGGTC
ATAGAGACATCCCTAAGCAGTGCCAGTTTTTTGGCAAATGATGTGGACCTGCATTCCTTCCCATTTGAC
ACCTTTGGCAAAGGCTTGATCAAGAAGTGCCGGACGAGTCCCGATGCCTTCATCCAGCTGGCACTGCAG
CTCGCACATTACAAGGACATGGGCAAGTTCTGCCTCACGTATGAGGCTTCCATGACTCGGCTCTTCCGA
GAGGGGAGGACAGAGACTGTACGCTCCTGCACTACGGAGTCCTGCAACTTTGTGCTGGCCATGATGGAC
CCCACAACAACGGCAGAGCAGAGGTTCAAGCTGTTCAAGATAGCTTGTGAAAAGCACCAGCACCTGTAC
CGCCTCGCCATGACGGGCGCTGGCATCGACCGCCACCTCTTCTGCCTCTATGTGGTGTCCAAGTATCTG
GCAGTCGACTCACCTTTCCTGAAGGAGGTACTGTCTGAGCCATGGAGGTTGTCCACGAGCCAGACTCCT
CAGCAGCAGGTGGAACTGTTTGACTTTGAGAAATACCCTGACTATGTGTCCTGTGGCGGGGGCTTTGGG
CCGGTTGCTGATGACGGCTATGGTGTTTCCTACATTATTGTGGGAGAGAATTTCATCCACTTCCATATT
TCTTCCAAGTTCTCTAGCCCTGAGACAGACTCACACCGCTTTGGGAAGCACTTGAGACAAGCCATGATG
GACATTATCACCTTGTTTGGCCTCACCGCCAATTCCAAAAAG
ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAAAC
Protein Sequence
>Peptide sequence encoded by MG210568
Blue=ORF Red=Cloning site Green=Tag(s)

MAEAHQAVAFQFTVTPDGIDLRLSHEALKQICLSGLHSWKKKFIRFKNGIITGVFPASPSSWLIVVVGV
ISSMHTKVDPSLGMIAKINRTLDTTGRMSSQTKNIVSGVLFGTGLWVAIIMTMRYSLKVLLSYHGWMFA
EHGKMSRSTRIWMAMVKVFSGRKPMLYSFQTSLPRLPVPAVKDTVSRYLESVRPLMKEGDFQRMTALAQ
DFAVNLGPKLQWYLKLKSWWATNYVSDWWEEYIYLRGRGPIMVNSNYYAMEMLYITPTHIQAARAGNTI
HAILLYRRTVDREELKPIRLLGSTIPLCSAQWERLFNTSRIPGEETDTIQHVKDSRHIVVYHRGRYFKV
WLYHDGRLLRPRELEQQMQQILDDTSEPQPGEAKLAALTAADRVPWAKCRQTYFARGKNKQSLDAVEKA
AFFVTLDESEQGYREEDPEASIDSYAKSLLHGRCFDRWFDKSITFVVFKNSKIGINAEHSWADAPIVGH
LWEYVMATDVFQLGYSEDGHCKGDKNPNIPKPTRLQWDIPGECQEVIETSLSSASFLANDVDLHSFPFD
TFGKGLIKKCRTSPDAFIQLALQLAHYKDMGKFCLTYEASMTRLFREGRTETVRSCTTESCNFVLAMMD
PTTTAEQRFKLFKIACEKHQHLYRLAMTGAGIDRHLFCLYVVSKYLAVDSPFLKEVLSEPWRLSTSQTP
QQQVELFDFEKYPDYVSCGGGFGPVADDGYGVSYIIVGENFIHFHISSKFSSPETDSHRFGKHLRQAMM
DIITLFGLTANSKK
TRTRPLEMESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKMKSTKGALTFSPYLLSHV
MGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRYEAGRVIGDFKVMGTGFPED
SVIFTDKIIRSNATVEHLHPMGDNDLDGSFTRTFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFA
FRRVEEDHSNTELGIVEYQHAFKTPDADAGEERV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013495
ORF Size 2319 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013495.2, NP_038523.2
RefSeq Size 4256 bp
RefSeq ORF 2322 bp
Locus ID 12894
UniProt ID P97742
Cytogenetics 19 3.08 cM
MW 88.3 kDa
Summary Catalyzes the transfer of the acyl group of long-chain fatty acid-CoA conjugates onto carnitine, an essential step for the mitochondrial uptake of long-chain fatty acids and their subsequent beta-oxidation in the mitochondrion. Plays an important role in triglyceride metabolism.UniProtKB/Swiss-Prot Function
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Resources
"NM_013495" in other vectors (4)
SKU Description Size Price
MC205196 Cpt1a (untagged) - Mouse carnitine palmitoyltransferase 1a, liver (Cpt1a), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$709.00
MR210568 Cpt1a (Myc-DDK-tagged) - Mouse carnitine palmitoyltransferase 1a, liver (Cpt1a), nuclear gene encoding mitochondrial protein 10 ug
$589.00 $708.00
MR210568L3 Lenti ORF clone of Cpt1a (Myc-DDK-tagged) - Mouse carnitine palmitoyltransferase 1a, liver (Cpt1a), nuclear gene encoding mitochondrial protein 10 ug
$1,008.00
MR210568L4 Lenti ORF clone of Cpt1a (mGFP-tagged) - Mouse carnitine palmitoyltransferase 1a, liver (Cpt1a), nuclear gene encoding mitochondrial protein 10 ug
$1,008.00

Plasmid Map for MG210568

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG210568
Circular map for MG210568

file-alt Citations

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