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Polk (BC052820) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG210382
Polk (tGFP-tagged) - Mouse polymerase (DNA directed), kappa (cDNA clone MGC:60610 IMAGE:30057338)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $877.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Polk
Synonyms Dinb1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG210382 representing BC052820
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATAACACAAAGGAAAAGGACAACTTCAAAGACGACCTCCTGCTCCGCATGGGACTAAACGATAACA
AAGCAGGCATGGAAGGGTTGGATAAGGAGAAAATTAACAAAATTATCATGGAAGCCACAAAGGGGTCCAG
ATTTTATGAATATGATCCCAATTTTATGGCCATGAGTCTGGACGAAGCCTACTTGAATATAACACAGCAC
TTGCAGGAAAGGCAAGATTGGCCTGAGGACAAAAGAAGATACTTCATCAAAATGGGAAACTACTTAAAAA
TCGACACACCCAGACAGGAAGCTAACGAGCTGACTGAGTATGAGCGGTCCATCTCCCCGCTGCTTTTTGA
AGATAGTCCTCCTGATTTGCAACCCCAAGGAAGTCCTTTCCAACTGAACTCTGAAGAACAAAACAATCCT
CAAATAGCCCAAAATTCAGTTGTTTTTGGAACATCAGCTGAGGAAGTGGTAAAGGAAATTCGCTTCAGAA
TTGAACAAAAAACAACGCTGACAGCCAGCGCAGGCATCGCCCCCAATACAATGTTAGCAAAAGTGTGCAG
TGATAAGAATAAGCCAAACGGACAGTACCAGATCCTTCCCAGCAGGAGCGCGGTGATGGACTTCATCAAG
GACCTGCCTATTAGAAAGGTTTCTGGGATAGGAAAAGTTACAGAGAAAATGTTAATGGCTCTCGGGATTG
TTACTTGCACAGAACTCTACCAACAGAGAGCGTTGCTGTCTCTCCTTTTCTCTGAAACCTCTTGGCATTA
TTTTCTTCACATCGCGCTGGGTCTAGGTTCAACAGACCTGGCAAGGGATGGAGAAAGGAAAAGCATGAGT
GTTGAAAGGACATTCAGTGAGATAAGTAAGACAGAGGAACAGTACAGCCTGTGCCAAGAACTGTGCGCTG
AGCTCGCCCACGACCTCCAGAAGGAAGGACTTAAGGGAAGAACCGTCACCATTAAGCTGAAGAACGTGAA
TTTTGAAGTAAAAACTCGTGCATCTACCGTTCCGGCCGCCATTTCTACTGCAGAGGAAATATTTGCCATT
GCCAAGGAGCTGCTAAGGACAGAAGTTAATGTGGGTTCTCCACACCCCCTGCGGTTAAGACTGATGGGTG
TCCGAATGTCTACTTTTTCCAGTGAAGATGACAGGAAACACCAACAAAGGAGCATCATTGGTTTCTTACA
AGCTGGAAACCAAGCTTTGTCATCTACTGGGGGTAGTCTAGACAAAACTGCCAAAACTGAGCTTGCAAAG
CCCTTAGAAATGTCTCATAAGAAGAGTTTCTTTGATAAAAAGCGATCAGAAAGAATCTCCAACTGTCAAG
ACACATCCAGATGTAAAACTGCGGGTCAGCAAGCTTTACAGATCTTGGAACCATCCCAAGCATTAAAGAA
GCTGAGCCAGAGTTTTGAAACATCAGAGAATTCAAATGACTGTCAGACATTTATATGTCCAGTTTGCTTT
AGGGAGCAAGAAGGTGTCAGTCTGGAAGCCTTTAATGAACATGTAGATGAGTGTCTTGATGGACCGTCAA
CCAGTGAGAACTCAAAAATATCCTGTTACTCACATGCTTCCTCTGCAGACATTGGTCAGAAGGAAGATGT
ACACCCCTCTATTCCACTGTGTGAGAAACGGGGGCATGAAAATGGAGAGATCACTTCAGTAGATGGTGTA
GATCTAACAGGGACGGAAGACAGATCATTGAAAGCAGCAAGTATGGACACTCTAGAGAATAATCGCAGCA
AAGAGGAATGTCCTGATATTCCAGACAAGTCTTGTCCTATATCACTGGCAAATGAAACCATCAGTACATT
AAGTAGGCAAGAATCTGTCCAGCCTTGTACAGATGAGGTAGTAACAGGACGAGCTCTAGTGTGTCCTGTT
TGTAACCTAGAACAAGAGACTTCTGATCTTACCCTCTTCAACATACATGTGGATATTTGCTTAAATAAAG
GTATTATCCAAGAACTGAGAAATAGTGAAGGTAATTCAGTTAAACAACCCAAAGAAAGCTCGAGAAGTAC
TGACAGACTTCAGAAGGCTTCAGGAAGGACCAAAAGGCCACGTGGAGCAACCTCACCATCAGCTCCTCCA
TGCTTAGCCACCATGGTACCATTGATGCTTCCGTACCTACCTGTGTGCATCTCTGTGTCCTGTGAGCAGA
TGCAGTTAGAATTGGCCATGATGTCCATGAAGAACATTAGCTTAACA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG210382 representing BC052820
Red=Cloning site Green=Tags(s)

MDNTKEKDNFKDDLLLRMGLNDNKAGMEGLDKEKINKIIMEATKGSRFYEYDPNFMAMSLDEAYLNITQH
LQERQDWPEDKRRYFIKMGNYLKIDTPRQEANELTEYERSISPLLFEDSPPDLQPQGSPFQLNSEEQNNP
QIAQNSVVFGTSAEEVVKEIRFRIEQKTTLTASAGIAPNTMLAKVCSDKNKPNGQYQILPSRSAVMDFIK
DLPIRKVSGIGKVTEKMLMALGIVTCTELYQQRALLSLLFSETSWHYFLHIALGLGSTDLARDGERKSMS
VERTFSEISKTEEQYSLCQELCAELAHDLQKEGLKGRTVTIKLKNVNFEVKTRASTVPAAISTAEEIFAI
AKELLRTEVNVGSPHPLRLRLMGVRMSTFSSEDDRKHQQRSIIGFLQAGNQALSSTGGSLDKTAKTELAK
PLEMSHKKSFFDKKRSERISNCQDTSRCKTAGQQALQILEPSQALKKLSQSFETSENSNDCQTFICPVCF
REQEGVSLEAFNEHVDECLDGPSTSENSKISCYSHASSADIGQKEDVHPSIPLCEKRGHENGEITSVDGV
DLTGTEDRSLKAASMDTLENNRSKEECPDIPDKSCPISLANETISTLSRQESVQPCTDEVVTGRALVCPV
CNLEQETSDLTLFNIHVDICLNKGIIQELRNSEGNSVKQPKESSRSTDRLQKASGRTKRPRGATSPSAPP
CLATMVPLMLPYLPVCISVSCEQMQLELAMMSMKNISLT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC052820
ORF Size 2217 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC052820, AAH52820
RefSeq Size 3517 bp
RefSeq ORF 2219 bp
Locus ID 27015
Cytogenetics 13 D1
Summary DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls (PubMed:12432099). Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity (By similarity).UniProtKB/Swiss-Prot Function
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"BC052820" in other vectors (4)
SKU Description Size Price
MC220775 Polk (untagged) - Mouse polymerase (DNA directed), kappa (cDNA clone MGC:60610 IMAGE:30057338), (10ug) 10 ug
$745.00
MR210382 Polk (Myc-DDK-tagged) - Mouse polymerase (DNA directed), kappa (cDNA clone MGC:60610 IMAGE:30057338) 10 ug
$589.00 $677.00
MR210382L3 Lenti ORF clone of Polk (Myc-DDK-tagged) - Mouse polymerase (DNA directed), kappa (cDNA clone MGC:60610 IMAGE:30057338) 10 ug
$977.00
MR210382L4 Lenti ORF clone of Polk (mGFP-tagged) - Mouse polymerase (DNA directed), kappa (cDNA clone MGC:60610 IMAGE:30057338) 10 ug
$977.00

Plasmid Map for MG210382

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG210382
Circular map for MG210382

file-alt Citations

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