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Sfi1 (BC046305) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG210256
Sfi1 (tGFP-tagged) - Mouse Sfi1 homolog, spindle assembly associated (yeast) (cDNA clone MGC:54840 IMAGE:6493676)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $857.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sfi1
Synonyms mKIAA0542, RP23-191E3.10
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG210256 representing BC046305
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGAAGAAGATTGGTTCCAGGTCTTTTAGGGATGGTGTGGTTAAGAAACCTTGTTCTCCAAAGACCC
TGCCCCTCAAAAAGTCTTCTGCCTTCTCTGGGATCCAGAGGGAGCCATCTAGAAGCTGCCACTCTATATA
CTATCATGCCTCACAAAACTGGACTCGGTACCGATTACAAGAACTTCGAATCAGGTGTGTGGCCAGAAAG
TTCCTGTATTTGTGGATTCGAGTGACTTTTGGAAGAGTGACTCCCTCTAGAGCCAGGATTTTCCATGAGC
AGAAAATATTACAGAAGGTCTTCGGAGAATGGAGGGAAGAATGGTGGGTCTCCCAGAGGGAGTGGAAGCT
CTGTGTCCGAGCTGACTGTCACTACAGATACTACTTGTACAACCTGATTTTCCAGAACTGGAAGACCTTT
GTGCACCAGCAACGGGAGATGAGGAAGAGGTTCCGAATAGCTGAGCATCATGATACGAAGCAGAAGATGT
GCCAAGCCTGGAAGTCCTGGTTGATATACATGGTTTCTCGTAGGACCAAACTTCACATGAAGACTACTGC
TCTAGAGTTTAGGAGGCAGAGTGTCTTATGGGTGACAGTCCTACACAAGTGTGTCAGGGTGTGGCTACGG
TATGTGCACAAGAGACAATGGCAGCAGTTATTGCGGGCCAGAGCAGATGGTCATTTCCAGCAGCGAGCAC
TGCCTGCTGCCTTCTACACGTGGTACAGAGGCTGGCTATGGCACCAGCAGAGACGGATCCTGCACACAAA
AGCAGTGCGTTTCCACAGAGGGACATTAGAGAAGCAAGTATTTGCTCTCTGGAGGCAGAAGATGTCTCAG
CATCGAGAAAACTGCTTGGCAGAGAGAATGGCTATACTGCAGGCAGAGCAGCAGCTTTTGCGTAGGTTCT
GGTTCGTGTGGCACCAGCAGGCAGCAGTGTGTCAGCTGGAGCGACAGCAGCAAGCCATGGCCATTGCCCA
CCACCACAGTGGACTGCTCAGGAGGGCTTTCTGCATCTGGAAGGAAAGCACCCAAGGATTTAGGATAGAG
AGGATGGGCAGGGCACAGGCTGCGCACTTCCACTCTGCACAGCTCTTGAGTCGGGCCTGGAGCATGTGGA
GGGAGGTTTACCAGAATAGGGTGAGGAGTGTTCTGCGGGAGGTGGCGGCCAGGGAGAGACAGCACAACAG
GCAGCTGCTGTGGTGGGCGTTACATCTCTGGAGAGAAAACACCATGGCCCGTTTGGATGGAGCCAAAAAA
ACCTCTCAAGCAAGAGTTCACTACAGCAGGACTCTGTGCTCCAAGGTCCTCGTCCAGTGGCGAGAGGTTA
CTTCAGTGCAGATATATTACCGACAAAAGGAGGCAGCTGCCCTCAGGGAGGCCCGGAAAGCACTGGACAG
GGGTCGTCTCCAGAACTGGTTTCAGCACTGGCGATTCTGCAGCCAGAGGGCAGCCCAGCAGAGATTCCAG
CTGGGACAGGCGGCTCAGCATCACCACTGGCAGCTGCTGATGGAAGCAATGGCCAGGTGGAAGGCACACC
ATCTGGGGTGTATCAGAAAGAAGTTTCTGCAGAGGCAAGCTGCCCAACTCCTGGCACAGAGACTCAGTCG
GGCCTGCTTCTGCCAGTGGAGGAAGCAGCTGGCAGTCCGGAAGCAAGAACAGTGGGGCACAGCCCGGGCC
CTGTGGCTCTGGGCCTTCTCACTACAAGCAAAGGTGTGGACTGCATGGCTGGGCTTCGTACTTGAGAGGA
GGAGGAAGAAGGCACGGCTGGAACGGGCTATGCAAGCCTACCAGCAGCAGCTCCTACAGGAGGGTGCCAC
ACGGCTTCTGCGCTTCACAGCTGGTACGAAGGCCTTCCGGCAGCAGCTCCAGGCCCAGCAGCAGGTCCAG
GCAGCCCACAGCCTCCACTGTGCAGTCCGCCACTGTGCTGAGCTCTGGAAAAAGAAGGTGCTTGGTCCAG
GCAAGACTTCTCAACCACCAGCACCCACCACATTCAGCAAGAGAGTGACATTTAAGGATTCTTTCCTCTC
TGGACATGCTGCGGAGGCTGGAGATGCTACCCAGGAGACAAAGAAGCTTCGAGCTCCTCCATCTCAGGGA
GTCCTGGGTAGTTTGGCAGGAGCTGCTGGGGAGCCCTGCCACCTAGACCTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG210256 representing BC046305
Red=Cloning site Green=Tags(s)

MEKKIGSRSFRDGVVKKPCSPKTLPLKKSSAFSGIQREPSRSCHSIYYHASQNWTRYRLQELRIRCVARK
FLYLWIRVTFGRVTPSRARIFHEQKILQKVFGEWREEWWVSQREWKLCVRADCHYRYYLYNLIFQNWKTF
VHQQREMRKRFRIAEHHDTKQKMCQAWKSWLIYMVSRRTKLHMKTTALEFRRQSVLWVTVLHKCVRVWLR
YVHKRQWQQLLRARADGHFQQRALPAAFYTWYRGWLWHQQRRILHTKAVRFHRGTLEKQVFALWRQKMSQ
HRENCLAERMAILQAEQQLLRRFWFVWHQQAAVCQLERQQQAMAIAHHHSGLLRRAFCIWKESTQGFRIE
RMGRAQAAHFHSAQLLSRAWSMWREVYQNRVRSVLREVAARERQHNRQLLWWALHLWRENTMARLDGAKK
TSQARVHYSRTLCSKVLVQWREVTSVQIYYRQKEAAALREARKALDRGRLQNWFQHWRFCSQRAAQQRFQ
LGQAAQHHHWQLLMEAMARWKAHHLGCIRKKFLQRQAAQLLAQRLSRACFCQWRKQLAVRKQEQWGTARA
LWLWAFSLQAKVWTAWLGFVLERRRKKARLERAMQAYQQQLLQEGATRLLRFTAGTKAFRQQLQAQQQVQ
AAHSLHCAVRHCAELWKKKVLGPGKTSQPPAPTTFSKRVTFKDSFLSGHAAEAGDATQETKKLRAPPSQG
VLGSLAGAAGEPCHLDL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC046305
ORF Size 2151 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC046305, AAH46305
RefSeq Size 3722 bp
RefSeq ORF 2153 bp
Locus ID 78887
Cytogenetics 11 A1
Summary Plays a role in the dynamic structure of centrosome-associated contractile fibers via its interaction with CETN2.UniProtKB/Swiss-Prot Function
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"BC046305" in other vectors (4)
SKU Description Size Price
MC220860 Sfi1 (untagged) - Mouse Sfi1 homolog, spindle assembly associated (yeast) (cDNA clone MGC:54840 IMAGE:6493676), (10ug) 10 ug
$723.00
MR210256 Sfi1 (Myc-DDK-tagged) - Mouse Sfi1 homolog, spindle assembly associated (yeast) (cDNA clone MGC:54840 IMAGE:6493676) 10 ug
$589.00 $657.00
MR210256L3 Lenti ORF clone of Sfi1 (Myc-DDK-tagged) - Mouse Sfi1 homolog, spindle assembly associated (yeast) (cDNA clone MGC:54840 IMAGE:6493676) 10 ug
$957.00
MR210256L4 Lenti ORF clone of Sfi1 (mGFP-tagged) - Mouse Sfi1 homolog, spindle assembly associated (yeast) (cDNA clone MGC:54840 IMAGE:6493676) 10 ug
$957.00

Plasmid Map for MG210256

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG210256
Circular map for MG210256

file-alt Citations

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