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Abcf3 (NM_013852) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG210185
Abcf3 (tGFP-tagged) - Mouse ATP-binding cassette, sub-family F (GCN20), member 3 (Abcf3)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $850.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Abcf3
Synonyms AI326318; AU016058; BB119416
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG210185 representing NM_013852
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGACTTGCGCTGATATCCTGCGAAGCGAGTTCCCTGAAATTGACGGGCAGGTCTTCGACTACGTGA
CCGGTGTCTTGCACAGTGGCAGCGCGGATTTCGAGTCTGTGGATGACCTGGTGGAAGCTGTCGGGGAACT
ATTGCAAGAAGTGTCCGGGGACAGCAAGGATGACGCTGGCATTAGGGCTGTCTGTCAGCGCATGTACAAC
ACTCTACGCCTGGCTGAACCGCAGAACCAGGGAAACAGCCAGGTGCTACTGGACGCCCCCATCCAGTTGT
CGAAGATAATGGAAAACTATGACTGTGACACCAAACTTCCAGGACTGCTAAAGAGGGAACAGTCCTCGAC
AGTAAATGCGAAGAAACTAGAGAAGGCTGAAGCTCGACTGAAGGCAAAGCAGGAGAAGCGTTCAGAGAAG
GAAACACTGAAGACCAGCAACCCTCTAGTCTTGGAGGAGGCGTCAGCTAGCCAGGCAGGCAGCAGAAAAG
AGAGTCGATTGGAATCATCTGGCAAGAACAAGTCCTATGATGTGCGAATTGAGAACTTTGATGTCTCTTT
TGGTGACAGGGTACTGCTGGCTGGAGCAGATGTAAACTTGGCATGGGGCCGACGCTACGGCCTGGTGGGA
CGCAATGGTCTGGGGAAGACGACATTGCTAAAGATGCTGGCTACCCGGAGCTTGCGTGTCCCAGCCCATA
TTTCCCTACTGCATGTAGAGCAGGAAGTTGCTGGAGATGACACTCCTGCCTTGCAGAGTGTGCTGGAAAG
TGACACCGTACGAGAGGACCTGCTACGGCAGGAACGGGAGCTCAGCCTCAGGATTGCCGCTGGCAGGGCA
GAAGGTTCAGAAGCTGCACAGCTGGCAGAAATCTATGGGAAGCTGGAGGAGATTGAGGCAGATAAGGCAC
CTGCCAGAGCATCAGTCATTCTTGCGGGCCTTGGTTTTACTCCTAAAATGCAGCAGCAGCCTACCAGGGA
GTTCTCAGGTGGCTGGAGGATGAGATTGGCCTTAGCCCGAGCTCTCTTTGCCAGGCCAGACCTTCTGCTG
TTAGACGAACCCACAAACATGCTGGATGTAAGGGCCATCCTGTGGTTGGAGAATTACCTGCAGACGTGGC
CCTCCACTATCCTAGTTGTCTCTCATGACCGAAACTTCCTGAATGCCATTGCCACAGACATCATCCACTT
GCATAGCCAACGGCTAGACGGTTACCGGGGGGACTTTGAAACCTTCATCAAGAGCAAGCAGGAGCGCCTT
CTCAACCAGCAGCGCGAGTATGAGGCCCAGCAGCAGTATCGCCAACACATCCAGGTTTTCATCGACCGTT
TTCGATACAATGCAAACAGAGCCTCTCAAGTGCAGAGTAAACTGAAGATGTTGGAGAAGCTACCTGAACT
AAAACCGGTGGACAAGGAATCCGAGGTGGTGTTGAAGTTCCCCGATGGATTTGAGAAGTTCTCACCGCCC
ATTCTGCAGCTAGATGAGGTGGATTTCTACTATGATCCTAAGCACAGCATCTTCAGTCGCCTGTCTGTCT
CTGCTGATCTTGAGTCCCGCATCTGTGTGGTTGGGGAGAATGGGGCTGGGAAATCTACTATGCTGAAGCT
GCTCATGGGGGACCTGTCTCCTGTTCGGGGTATCAGGCATGCCCACAGGAATCTGAAGATAGGCTATTTT
AGCCAGCACCACGTGGAACAGCTAGACTTGAATGTCAGTGCTGTGGAACTCCTGGCTCGAAAATTTCCTG
GGCTACCTGAAGAGGAATATCGTCACCAATTGGGCCGGTATGGCATCTCTGGAGAACTGGCCATGCGTCC
TGTTGCGAGCTTATCTGGGGGCCAGAAAAGCCGGGTAGCCTTTGCTCAGATGACCATGCCTTGCCCCAAC
TTCTATATTCTGGATGAACCCACAAACCACCTGGACATGGAGACCATTGAAGCTTTGGGCCAGGCTCTCA
ACAACTTCAGGGGTGGTGTAATTCTGGTATCTCATGATGAGCGCTTCATCCGACTGGTGTGCAAGGAGCT
GTGGGTGTGTGAGAACGGCAGTGTCACTCGGGTTGAGGGGGGCTTTGACCAGTACCGGGCCCTCCTCCAA
GAACAGTTCCGCCGGGAGGGCTTCCTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG210185 representing NM_013852
Red=Cloning site Green=Tags(s)

MATCADILRSEFPEIDGQVFDYVTGVLHSGSADFESVDDLVEAVGELLQEVSGDSKDDAGIRAVCQRMYN
TLRLAEPQNQGNSQVLLDAPIQLSKIMENYDCDTKLPGLLKREQSSTVNAKKLEKAEARLKAKQEKRSEK
ETLKTSNPLVLEEASASQAGSRKESRLESSGKNKSYDVRIENFDVSFGDRVLLAGADVNLAWGRRYGLVG
RNGLGKTTLLKMLATRSLRVPAHISLLHVEQEVAGDDTPALQSVLESDTVREDLLRQERELSLRIAAGRA
EGSEAAQLAEIYGKLEEIEADKAPARASVILAGLGFTPKMQQQPTREFSGGWRMRLALARALFARPDLLL
LDEPTNMLDVRAILWLENYLQTWPSTILVVSHDRNFLNAIATDIIHLHSQRLDGYRGDFETFIKSKQERL
LNQQREYEAQQQYRQHIQVFIDRFRYNANRASQVQSKLKMLEKLPELKPVDKESEVVLKFPDGFEKFSPP
ILQLDEVDFYYDPKHSIFSRLSVSADLESRICVVGENGAGKSTMLKLLMGDLSPVRGIRHAHRNLKIGYF
SQHHVEQLDLNVSAVELLARKFPGLPEEEYRHQLGRYGISGELAMRPVASLSGGQKSRVAFAQMTMPCPN
FYILDEPTNHLDMETIEALGQALNNFRGGVILVSHDERFIRLVCKELWVCENGSVTRVEGGFDQYRALLQ
EQFRREGFL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013852
ORF Size 2127 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013852.2, NP_038880.1
RefSeq Size 3288 bp
RefSeq ORF 2130 bp
Locus ID 27406
UniProt ID Q8K268
Cytogenetics 16 12.46 cM
Summary Displays an antiviral effect against flaviviruses such as west Nile virus (WNV) in the presence of OAS1B.UniProtKB/Swiss-Prot Function
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"NM_013852" in other vectors (4)
SKU Description Size Price
MC201378 Abcf3 (untagged) - Mouse ATP-binding cassette, sub-family F (GCN20), member 3 (Abcf3), (10ug) 10 ug
$650.00
MR210185 Abcf3 (Myc-DDK-tagged) - Mouse ATP-binding cassette, sub-family F (GCN20), member 3 (Abcf3) 10 ug
$589.00 $650.00
MR210185L3 Lenti ORF clone of Abcf3 (Myc-DDK-tagged) - Mouse ATP-binding cassette, sub-family F (GCN20), member 3 (Abcf3) 10 ug
$950.00
MR210185L4 Lenti ORF clone of Abcf3 (mGFP-tagged) - Mouse ATP-binding cassette, sub-family F (GCN20), member 3 (Abcf3) 10 ug
$950.00

Plasmid Map for MG210185

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG210185
Circular map for MG210185

file-alt Citations

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