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Kifc1 (BC003753) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG209950
Kifc1 (tGFP-tagged) - Mouse kinesin family member C1 (cDNA clone MGC:5880 IMAGE:3495308)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
$789.00 $817.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Kifc1
Synonyms HSET, KNSL2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG209950 representing BC003753
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGTGCAGGCGCAGAGGCCACCTTTGTTGGAAGTGAAGAGGAACGTAGAACTGAAGGCAGCCCTGG
TGAAGTCCTCCTCCCGAGTGCCCCTGTCAGCAAGCAGGCTCAAGAGGGGTCCTGACCAGATGGAGGATGC
CTTGGAGCCTGCAAAGAAACGGACACGAGTCATGGGTGCAGTGACCAAAGTTGACACATCCCGTCCCAGA
GGACCACTCCTCAGCACAGTGTCACAGACCCAGGGCCATACTGCAGCTCAGAAAGGCCCTAAGAAGACAG
GACCTCGTGGGTGCTCTGCTGTTGGTACAGTGCTGAGGAGCCAGAAGCCAGCTCCCGCTGCTCCTGCCCA
GAAGCCTGGCACATCCACTGCTCCTGTGGTGGTAGGGAAGAGAGCTGGCAAACGCCCTGCCTGGGACCTG
AAGGGCCAGTTGTGTGACCTCAATGAAGAGTTGAAACGCTATCGGGAGAAGACTCAAACGCTGGAACTGG
AGAACCGGGGGCTTCGGGAGCAACTCAGAGAGGTCCAGGAGCAGGCCACGACCCTGGGGACAGAGCGGAA
CACCCTGGAAGGGGAGCTGGCCAGTGTACGCAGCCGAGCTGAGCAGGACCAGCAGAGGCTGGAGACGCTG
AGTGCCCGTGTCTTGGAGCTGGAGGAATGTCTGGGTACCAGGGAAAGGCTGCTTCAGGAGCTTCAGGGAG
AGCGGCTGCAATTGCAGGAAGAGCGGAGCACACTGAGCACCCAGCTGGAGGAGCAGAAGAGGAGGTTTCA
GGCCACAGAAGCAGCTCTGTCAAGCAGCCAAGAAGAGGTGGTGTGTCTTCGGCAGAAGACTGAAGCCCAG
GTGACCTTACTGGCTGAGCAAGGAGACCGGCTCTATGGGTTAGAGATGGAGCGGCGGCGACTCCACAACC
AGCTGCAGGAACTGAAGGGCAATATCCGGGTGTTCTGCCGTGTGCGCCCTGTCCTCGCAGGGGAATCCAC
TCCATCTCCTGGCTTCCTCGTGTTTCCTCCTGGCCCTGCTGGACCCTCTGATCCCCCGACGGGCCTTAGC
CTCTCACGATCTGATGATCGGCGCTCCACCCTGACTGGGGCCCCGGCCCCCACTGTCCGCCATGATTTCT
CCTTTGATCGGGTGTTCCCGCCGGGAAGCAAGCAGGAGGAAGTGTTTGAGGAGATCGCCATGCTTGTCCA
GTCAGCACTGGATGGCTACCCTGTGTGCATTTTTGCCTATGGACAGACAGGCAGTGGCAAGACCTTCACT
ATGGAAGGAGGGCCTAGGGGAGACCCCCAATTGGAAGGGCTGATCCCTCGGGCCATGCGGCATCTGTTCT
CTGTGGCCCAGGAGATGAGCGGCCAGGGCTGGACATACAGTTTTGTGGCGAGTTACGTAGAGATCTACAA
TGAGACCGTTCGAGACCTGCTAGCTACTGGGCCCCGCAAGGGACAAGGGGGCGAGTGTGAGATCCGTCGG
GCAAGCCCAGGGAGTGAGGAGCTTACTGTCACCAATGCCCGCTATGTCCCTGTTTCCTGTGAGAAAGAGG
TGGAGGCCCTGCTCCATTTGGCTCATCAGAACCGGGCTGTGGCCCACACTGCCCAAAATAAGAGATCATC
ACGCAGTCATAGTGTGTTCCAGCTGCAGATTTCTGGAGAGCATGCAGCTCGGGGCCTGCAGTGTGGCGCT
CCCCTCAACCTTGTGGACCTAGCTGGGAGTGAGCGGCTAGACCCTGGCTTACACCTAGGCCCTGGGGAGC
GTGATCGTCTTCGGGAGACACAGGCCATTAACAGCAGTCTGTCTACACTGGGACTGGTCATAATGGCCCT
GAGCAATAAGGAGTCCCACGTGCCTTACCGAAACAGCAAGCTCACCTACTTGCTGCAGAACTCTCTGGGT
GGCAGTGCCAAGATGCTTATGTTTGTGAATATTTCTCCTCTGGAAGAGAATGTCTCCGAGTCTCTGAATT
CACTACGCTTTGCTTCCAAGGTGAACCAGTGTGTCATTGGTACTGCTCAGGCTAATAAGAAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG209950 representing BC003753
Red=Cloning site Green=Tags(s)

MDVQAQRPPLLEVKRNVELKAALVKSSSRVPLSASRLKRGPDQMEDALEPAKKRTRVMGAVTKVDTSRPR
GPLLSTVSQTQGHTAAQKGPKKTGPRGCSAVGTVLRSQKPAPAAPAQKPGTSTAPVVVGKRAGKRPAWDL
KGQLCDLNEELKRYREKTQTLELENRGLREQLREVQEQATTLGTERNTLEGELASVRSRAEQDQQRLETL
SARVLELEECLGTRERLLQELQGERLQLQEERSTLSTQLEEQKRRFQATEAALSSSQEEVVCLRQKTEAQ
VTLLAEQGDRLYGLEMERRRLHNQLQELKGNIRVFCRVRPVLAGESTPSPGFLVFPPGPAGPSDPPTGLS
LSRSDDRRSTLTGAPAPTVRHDFSFDRVFPPGSKQEEVFEEIAMLVQSALDGYPVCIFAYGQTGSGKTFT
MEGGPRGDPQLEGLIPRAMRHLFSVAQEMSGQGWTYSFVASYVEIYNETVRDLLATGPRKGQGGECEIRR
ASPGSEELTVTNARYVPVSCEKEVEALLHLAHQNRAVAHTAQNKRSSRSHSVFQLQISGEHAARGLQCGA
PLNLVDLAGSERLDPGLHLGPGERDRLRETQAINSSLSTLGLVIMALSNKESHVPYRNSKLTYLLQNSLG
GSAKMLMFVNISPLEENVSESLNSLRFASKVNQCVIGTAQANKK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC003753
ORF Size 2022 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC003753, AAH03753
RefSeq Size 2263 bp
RefSeq ORF 2024 bp
Locus ID 16580
Cytogenetics 17 A3.3
Summary Minus end-directed microtubule-dependent motor required for bipolar spindle formation (PubMed:16638812). May contribute to movement of early endocytic vesicles (PubMed:17360972). Regulates cilium formation and structure (PubMed:23807208).UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC003753" in other vectors (4)
SKU Description Size Price
MC220645 Kifc1 (untagged) - Mouse kinesin family member C1 (cDNA clone MGC:5880 IMAGE:3495308), (10ug) 10 ug
$680.00
MR209950 Kifc1 (Myc-DDK-tagged) - Mouse kinesin family member C1 (cDNA clone MGC:5880 IMAGE:3495308) 10 ug
$589.00 $617.00
MR209950L3 Lenti ORF clone of Kifc1 (Myc-DDK-tagged) - Mouse kinesin family member C1 (cDNA clone MGC:5880 IMAGE:3495308) 10 ug
$917.00
MR209950L4 Lenti ORF clone of Kifc1 (mGFP-tagged) - Mouse kinesin family member C1 (cDNA clone MGC:5880 IMAGE:3495308) 10 ug
$917.00

Plasmid Map for MG209950

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG209950
Circular map for MG209950

file-alt Citations

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