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Flrt1 (NM_201411) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG209947
Flrt1 (tGFP-tagged) - Mouse fibronectin leucine rich transmembrane protein 3 (Flrt1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $817.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Flrt1
Synonyms AW742165; D630040I23Rik
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG209947 representing NM_201411
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGGTGGCACACTCTGCTGCCACCGCTACCACCACACCTGCTGCCACGGTCACAGCCACTGTCGTGA
TGACCACAGCCACCATGGACCTGCGGGACTGGCTGTTTCTCTGTTATGGGCTCATTGCCTTCCTCACGGA
GGTCATCGATAGCACCACCTGCCCATCAGTGTGCCGCTGTGACAATGGCTTCATCTACTGCAATGACCGG
GGACTCACATCCATCCCCTCAGACATCCCCGACGATGCCACCACCCTCTACCTACAGAACAACCAGATCA
ACAATGCGGGCATCCCTCAGGACCTCAAGACCAAGGTCAAGGTGCAGGTCATCTACCTGTATGAGAATGA
CCTGGACGAGTTCCCTATCAATCTCCCCCGCTCCCTGAGAGAGCTGCATCTGCAGGACAACAATGTGCGC
ACCATCGCCAGGGACTCTTTGGCTCGCATCCCGCTGCTGGAGAAGCTGCACCTGGACGACAACTCCGTTT
CCACCGTGAGCATCGAGGAGGATGCTTTTGCTGACAGCAAGCAGCTCAAGCTGCTTTTCCTGAGCCGGAA
CCACCTGAGCAGCATCCCGTCAGGGCTGCCCCACACGCTGGAGGAACTGAGGCTAGATGACAACCGCATC
TCTACCATCCCCCTGCACGCATTCAAGGGTCTCAACAGCCTGCGGCGCCTGGTTCTGGATGGCAACCTGC
TGGCCAACCAGCGCATTGCCGACGACACCTTCAGCCGGCTGCAGAACCTCACGGAGCTGTCGCTAGTACG
GAACTCGCTGGCTGCCCCGCCCCTTAATCTGCCCAGCGCCCACCTGCAGAAGCTCTACCTGCAGGACAAT
GCCATCAGTCACATTCCCTACAACACCCTGGCCAAGATGCGGGAGCTGGAGAGGTTGGACCTGTCCAACA
ACAACCTCACCACGCTGCCTCGGGGCCTGTTTGATGACCTGGGGAACCTGGCACAGCTGCTTCTCAGGAA
CAACCCCTGGTTCTGTGGCTGTAACCTCATGTGGCTGAGGGACTGGGTGAGGGCACGGGCTGCAGTGGTC
AATGTGCGGGGCCTCATGTGCCAGGGCCCTGAGAAGGTCCGGGGCATGGCCATCAAAGACATCACCAGCG
AGATGGATGAGTGCTTTGAGGCGGGGTCACAGGGCGGTGCTGCTAATGCAGCCGCCAAGACCACAGTCAG
CAACCATGCCTCTGCCACCACACCCCAGGGCTCTCTTTTTACCCTCAAGGCCAAGAGGCCAGGACTGCGC
CTCCCAGACTCCAACATTGACTACCCCATGGCCACTGGCGATGGCGCCAAGACATTGGTCATCCAGGTGA
AGCCACTGACGGCAGACTCTATCCGAATCACATGGAAGGCCATGCTACCCGCCTCTTCTTTCCGGCTCAG
TTGGCTACGTCTGGGCCATAGCCCGGCTGTGGGCTCTATAACAGAGACCCTGGTGCAGGGGGACAAGACA
GAGTACTTGCTGACAGCACTGGAGCCCAAGTCCACCTACATCATCTGTATGGTCACCATGGAGACTGGCA
ACACCTACGTGGCCGATGAGACACCTGTGTGTGCCAAGGCAGAGACAGCAGATAGCTATGGCCCTACCAC
CACGCTCAACCAGGAACAGAATGCTGGCCCTATGGCGGGGCTGCCCCTGGCTGGGATTATTGGTGGTGCC
GTGGCTCTTGTATTTCTCTTCCTGGTCCTGGGAGCCATTTGCTGGTACGTGCACCGGGCTGGCGAGCTGC
TGACCCGAGAGAGGGTCTACAACAGGGGCAGCAGGAGGAAGGACGACTACATGGAGTCAGGGACCAAGAA
GGATAACTCCATTCTAGAAATCCGTGGCCCAGGACTGCAGATGCTACCCATCAACCCGTACCGCTCCAAA
GAGGAGTACGTGGTGCACACCATATTTCCCTCCAATGGCAGCAGCCTCTGCAAGGGTGCCCACACTATTG
GCTATGGCACCACACGAGGCTACCGGGAAGCGGGCATCCCTGATGTGGACTACTCCTACACA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG209947 representing NM_201411
Red=Cloning site Green=Tags(s)

MVVAHSAATATTTPAATVTATVVMTTATMDLRDWLFLCYGLIAFLTEVIDSTTCPSVCRCDNGFIYCNDR
GLTSIPSDIPDDATTLYLQNNQINNAGIPQDLKTKVKVQVIYLYENDLDEFPINLPRSLRELHLQDNNVR
TIARDSLARIPLLEKLHLDDNSVSTVSIEEDAFADSKQLKLLFLSRNHLSSIPSGLPHTLEELRLDDNRI
STIPLHAFKGLNSLRRLVLDGNLLANQRIADDTFSRLQNLTELSLVRNSLAAPPLNLPSAHLQKLYLQDN
AISHIPYNTLAKMRELERLDLSNNNLTTLPRGLFDDLGNLAQLLLRNNPWFCGCNLMWLRDWVRARAAVV
NVRGLMCQGPEKVRGMAIKDITSEMDECFEAGSQGGAANAAAKTTVSNHASATTPQGSLFTLKAKRPGLR
LPDSNIDYPMATGDGAKTLVIQVKPLTADSIRITWKAMLPASSFRLSWLRLGHSPAVGSITETLVQGDKT
EYLLTALEPKSTYIICMVTMETGNTYVADETPVCAKAETADSYGPTTTLNQEQNAGPMAGLPLAGIIGGA
VALVFLFLVLGAICWYVHRAGELLTRERVYNRGSRRKDDYMESGTKKDNSILEIRGPGLQMLPINPYRSK
EEYVVHTIFPSNGSSLCKGAHTIGYGTTRGYREAGIPDVDYSYT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_201411
ORF Size 2022 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_201411.1
RefSeq Size 2847 bp
RefSeq ORF 2025 bp
Locus ID 396184
UniProt ID Q6RKD8
Cytogenetics 19 A
Summary Plays a role in fibroblast growth factor-mediated signaling cascades that lead to the activation of MAP kinases (PubMed:16872596, PubMed:20421966). Promotes neurite outgrowth via FGFR1-mediated activation of downstream MAP kinases. Promotes an increase both in neurite number and in neurite length (PubMed:20421966). May play a role in cell-cell adhesion and cell guidance via its interaction with ADGRL1/LPHN1 and ADGRL3 (PubMed:22405201).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_201411" in other vectors (4)
SKU Description Size Price
MC220310 Flrt1 (untagged) - Mouse fibronectin leucine rich transmembrane protein 1 (Flrt1), (10ug) 10 ug
$680.00
MR209947 Flrt1 (Myc-DDK-tagged) - Mouse fibronectin leucine rich transmembrane protein 1 (Flrt1) 10 ug
$589.00 $617.00
MR209947L3 Lenti ORF clone of Flrt1 (Myc-DDK-tagged) - Mouse fibronectin leucine rich transmembrane protein 1 (Flrt1) 10 ug
$917.00
MR209947L4 Lenti ORF clone of Flrt1 (mGFP-tagged) - Mouse fibronectin leucine rich transmembrane protein 1 (Flrt1) 10 ug
$917.00

Plasmid Map for MG209947

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG209947
Circular map for MG209947

file-alt Citations

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