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D14Ertd231e (BC055700) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG209823
D14Ertd231e (tGFP-tagged) - Mouse DNA segment, Chr 14, ERATO Doi 231, expressed (cDNA clone MGC:66665 IMAGE:6808816)
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ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $812.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol D14Ertd231e
Synonyms BC028953; D14Ertd231e
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG209823 representing BC055700
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAACTGTATTGCCTGTCAGGGCACCCAACTTTACCATGCAACGTACTCAAATTCAAATCCACTACTA
TTATGTTGGACTGTGGACTGGACATGACTTCCACCCTCAATTTCCTCCCTTTGCCTCTTGTTCAAAGCCC
CAGGCTATCTAATCTTCCCGGATGGTCTCTAAAAGACGGAAATGCTTTTTTGGACAAGGAGCTAAAAGAG
TGCTCCGGTCATGTATTTGTGGATTCTGTGCCTGAATTCTGCTTACCAGAGACCGAGCTAATAGACCTGT
CTACAGTCGATGTGATCCTCATCTCTAACTACCACTGCATGATGGCGCTGCCCTACATCACCGAGCACAC
AGGATTCACAGGCACAGTGTATGCCACAGAACCCACCATGCAGATCGGCAGGCTTCTCATGGAAGAGCTG
GTGAATTTCATCGAAAGAGTGCCAAAGGCCCAGTCTGCCTCCTTGTGGAAGAATAAGGATATACAGCGGC
TGCTGCCTTCTCCCCTCAAGGATGCAGTGGAAGTGTCAACCTGGAGAAGATGCTACACGATGCAGGAGGT
GAACTCGGCCCTCAGTAAAATCCAGCTGGTGGGATATTCCCAGAAAATCGAGCTGTTTGGTGCTGTCCAG
GTGACCCCTCTGAGCTCTGGCTATGCCCTTGGAAGCTCCAACTGGATTATCCAGTCACATTATGAGAAAG
TATCTTACGTCTCTGGATCCTCCTTGCTTACCACACACCCTCAGCCCATGGACCAAGCTTCTCTCAAAAA
CAGTGACGTTCTGATTCTCACAGGGCTGACACAGATCCCTACTGCAAACCCGGACGGCATGGTGGGCGAG
TTCTGCAGCAACCTCGCTCTGACAGTTCGGAATGGAGGAAACGTGTTGGTCCCTTGCTATCCTTCAGGCG
TGATCTATGACCTCCTGGAGTGCTTATATCAATACATTGACTCCGCTGGCCTCTCCAACATCCCTTTCTA
CTTCATCTCCCCCGTTGCCAACAGTTCACTCGAGTTTTCCCAGATTTTTGCTGAGTGGCTTTGTCACAAC
AAACAGAGTAAAGTTTACCTCCCAGAACCACCTTTCCCACATGCAGAGCTCATTCAGACGAATAAGCTGA
AGCACTACCGCAGCATCCACGGTGACTTCAGCAATGACTTCAGACAGCCGTGTGTGCTGTTCACCGGACA
TCCTTCCCTCCGCTTTGGGGACGTCGTCCACTTCATGGAGCTCTGGGGGAAGTCCAGCCTCAACACCATC
ATATTCACAGAACCCGATTTCTCCTACCTGGAGGCCTTGGCTCCTTACCAGCCGCTGGCCATGAAGTGCA
TCTACTGCCCAATTGACACTCGACTGAATTTCATCCAGGTGTCAAAGCTGCTTAAAGAAGTACAGCCCCT
GCACGTGGTCTGTCCTGAGCAGTACACCCAGCCACCGCCAGCCCAGGCCCACAGGATGGACCTCATGATC
GACTGCCAGCCACCAGCCATGTCCTATCGACGGGCTGAGGTCCTTGCCCTGCCCTTCAAACGACGCTATG
AGAAAATTGAAATCATGCCAGAGCTGGCAGACTCCCTGGTGCCCATGGAAATCAAGCCTGGCATCTCCTT
GGCGACTGTCTCAGCTGTGCTGCATACGAAGGATAACAAACATGTGCTTCAGCCCCCTCCCAAGCCCACC
CAGCCCACCAGCAGTAAGAAGAGGAAGCGGGTGAACGAGGACATTCCTGACTGCAAAGTACTGAAGCCTC
TGCTGAGTGGCTCCATCCCCGTAGAGCAGTTTGTGCAGACCCTGGAGAAGCATGGCTTCAGTGACATTAA
GGTGGAAGACACAGCCAAGGGACATATTGTCCTGCTGCAGGAAGCCGAGACACTCATCCAGATTGAAGAA
GACTCCACTCACATCATCTGTGACAATGACGAGACACTGAGGGTCCGACTGCGGGACCTTGTCCTCAGGT
TCCTGCAGAAGTTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG209823 representing BC055700
Red=Cloning site Green=Tags(s)

MKLYCLSGHPTLPCNVLKFKSTTIMLDCGLDMTSTLNFLPLPLVQSPRLSNLPGWSLKDGNAFLDKELKE
CSGHVFVDSVPEFCLPETELIDLSTVDVILISNYHCMMALPYITEHTGFTGTVYATEPTMQIGRLLMEEL
VNFIERVPKAQSASLWKNKDIQRLLPSPLKDAVEVSTWRRCYTMQEVNSALSKIQLVGYSQKIELFGAVQ
VTPLSSGYALGSSNWIIQSHYEKVSYVSGSSLLTTHPQPMDQASLKNSDVLILTGLTQIPTANPDGMVGE
FCSNLALTVRNGGNVLVPCYPSGVIYDLLECLYQYIDSAGLSNIPFYFISPVANSSLEFSQIFAEWLCHN
KQSKVYLPEPPFPHAELIQTNKLKHYRSIHGDFSNDFRQPCVLFTGHPSLRFGDVVHFMELWGKSSLNTI
IFTEPDFSYLEALAPYQPLAMKCIYCPIDTRLNFIQVSKLLKEVQPLHVVCPEQYTQPPPAQAHRMDLMI
DCQPPAMSYRRAEVLALPFKRRYEKIEIMPELADSLVPMEIKPGISLATVSAVLHTKDNKHVLQPPPKPT
QPTSSKKRKRVNEDIPDCKVLKPLLSGSIPVEQFVQTLEKHGFSDIKVEDTAKGHIVLLQEAETLIQIEE
DSTHIICDNDETLRVRLRDLVLRFLQKF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC055700
ORF Size 1974 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC055700, AAH55700
RefSeq Size 2414 bp
RefSeq ORF 1976 bp
Locus ID 210925
Cytogenetics 14 33.81 cM
Summary Component of the Integrator (INT) complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Mediates recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the INT complex.UniProtKB/Swiss-Prot Function
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"BC055700" in other vectors (4)
SKU Description Size Price
MC217972 D14Ertd231e (untagged) - Mouse DNA segment, Chr 14, ERATO Doi 231, expressed (cDNA clone MGC:66665 IMAGE:6808816), (10ug) 10 ug
$675.00
MR209823 D14Ertd231e (Myc-DDK-tagged) - Mouse DNA segment, Chr 14, ERATO Doi 231, expressed (cDNA clone MGC:66665 IMAGE:6808816) 10 ug
$289.00 $612.00
MR209823L3 Lenti ORF clone of D14Ertd231e (Myc-DDK-tagged) - Mouse DNA segment, Chr 14, ERATO Doi 231, expressed (cDNA clone MGC:66665 IMAGE:6808816) 10 ug
$912.00
MR209823L4 Lenti ORF clone of D14Ertd231e (mGFP-tagged) - Mouse DNA segment, Chr 14, ERATO Doi 231, expressed (cDNA clone MGC:66665 IMAGE:6808816) 10 ug
$912.00

Plasmid Map for MG209823

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG209823
Circular map for MG209823

file-alt Citations

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