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Jph4 (BC052049) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG209581
Jph4 (tGFP-tagged) - Mouse junctophilin 4 (cDNA clone MGC:62430 IMAGE:5689347)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $785.00
3 Weeks*
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Other products for "Jph4 or BC052049"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Jph4
Synonyms JP-4, JPHL1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG209581 representing BC052049
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCCCCGGGGGCAAGTTCGACTTTGACGACGGGGGCTGCTACGTGGGGGGCTGGGAGGCGGGGCGGG
CACATGGCTACGGCGTGTGCACCGGGCCCGGCGCCCAGGGAGAGTACAGCGGCTGCTGGGCTCACGGCTT
CGAGTCGCTGGGTGTCTTCACGGGGCCCGGCGGACACAGTTACCAGGGCCACTGGCAGCAGGGCAAGCGC
GAAGGGCTGGGCGTGGAGCGCAAGAGCCGCTGGACCTACCGCGGCGAGTGGCTGGGCGGGCTGAAGGGGC
GCAGCGGCGTATGGGAGAGCGTGTCCGGCCTGCGCTACGCCGGGCTCTGGAAGGACGGCTTCCAGGACGG
CTACGGCACCGAGACCTACTCGGATGGAGGCACTTACCAAGGCCAGTGGCAAGCCGGGAAACGCCACGGC
TACGGGGTGCGCCAAAGTGTACCCTACCACCAGGCGGCGCTTCTGCGCTCACCCCGCCGCACCTCCCTGG
ACTCCGGCCACAGCGACCCCCCGACGCCGCCTCCACCCCTACCCCTACCAGGAGATGAAGGAGGCAGCCC
AGCCTCCGGCTCCCGAGGCGGCTTCGTGCTGGCGGGGCCAGGGGACGCCGACGGGGCGTCTTCCCGTAAG
CGCACCCCAGCAGCAGGTGGATTCTTCCGCCGTTCACTGCTGCTCAGCGGGCTCCGGGCTGGTGGGCGCC
GCAGCTCCCTGGGCAGCAAGAGGGGATCTCTACGCAGCGAGGTGAGCAGCGAAGTGGGCAGTACAGGACC
CCCGGGCTCCGAGGCCAGCGGGCCCCCAATCCCAGCTCCACCTGCCCTCATCGAGGGCTCAGCCACTGAG
GTGTACGCCGGTGAGTGGCGTGCGGACCGGCGCAGTGGCTATGGAGTGAGCCAGAGATCCAACGGGCTGC
GCTACGAAGGCGAGTGGCTGGGCAACCGACGCCACGGATACGGGCGCACCACCCGGCCCGATGGCTCCCG
TGAGGAGGGCAAGTACAAGCGTAACCGGCTGGTACACGGGGGACGTGTCCGCAGCCTCCTGCCTCTGGCC
CTTAGACGGGGCAAAGTCAAGGAGAAAGTGGACAGGGCTGTCGAAGGTGCCCGTCGAGCTGTGAGTGCTG
CCCGCCAGCGCCAGGAAATTGCGGCTGCCAGGGCAGCAGACGCCCTCCTAAAAGCAGTGGCAGCCAGCAG
CGTCGCGGAGAAGGCTGTGGAGGCTGCGCGGATGGCCAAGCTGATAGCCCAGGATCTACAACCCATGTTA
GAGGCCCCAGGCCGCAGACCCAGACAGGACTCAGGAGGTTCTGACACAGAGCCTTTGGATGAGGACAGCC
CTGGGGTATACGAGAATGGACTGACCCCCTCAGAGGGCTCTCCGGAACTACCCAGCAGCCCCGCCTCCTC
CCACCAACCTTGGCGAGCCCCTGCCTGCCGGAGTCCACTGCCTCCTGGAGGGAACTGGGGCCCCTTCTCC
AGCCCTAAAGCTTGGCCCGAGGAGTGGGGGGGTCCTGGTGAGCAGGCAGAGGAACTAGCTGGCTATGAGG
CTGAGGATGAGGCTGGGGTGCAGGGTCCAGGACCAAGAGATGGTTCCCCACTTCTCGGAGGCTGCAGTGA
CAGTTCAGGAAGTCTTCGAGAGGAGGAGGGGGAAGACGAAGAGTCCTTGCCCCAACTGAGGGCCCCAGGA
GGCTCAGAGTCTGAGCCTGTCACCACGCCAGTTTTGAGGGGCCTGTCTTCAAGGGGTCCTGATGCTGGGT
GCCTGACAGAAGAGGTTGAGGAGCCTGCTCCCACCGAGAGGCCTGCCCAGCCGGGAGCTGCCAACCCCCT
GGTGGTGGGAGCCGTGGCCTTGCTGGACCTCAGCCTGGCCTTCCTGTTCTCCCAGCTCCTCACC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG209581 representing BC052049
Red=Cloning site Green=Tags(s)

MSPGGKFDFDDGGCYVGGWEAGRAHGYGVCTGPGAQGEYSGCWAHGFESLGVFTGPGGHSYQGHWQQGKR
EGLGVERKSRWTYRGEWLGGLKGRSGVWESVSGLRYAGLWKDGFQDGYGTETYSDGGTYQGQWQAGKRHG
YGVRQSVPYHQAALLRSPRRTSLDSGHSDPPTPPPPLPLPGDEGGSPASGSRGGFVLAGPGDADGASSRK
RTPAAGGFFRRSLLLSGLRAGGRRSSLGSKRGSLRSEVSSEVGSTGPPGSEASGPPIPAPPALIEGSATE
VYAGEWRADRRSGYGVSQRSNGLRYEGEWLGNRRHGYGRTTRPDGSREEGKYKRNRLVHGGRVRSLLPLA
LRRGKVKEKVDRAVEGARRAVSAARQRQEIAAARAADALLKAVAASSVAEKAVEAARMAKLIAQDLQPML
EAPGRRPRQDSGGSDTEPLDEDSPGVYENGLTPSEGSPELPSSPASSHQPWRAPACRSPLPPGGNWGPFS
SPKAWPEEWGGPGEQAEELAGYEAEDEAGVQGPGPRDGSPLLGGCSDSSGSLREEEGEDEESLPQLRAPG
GSESEPVTTPVLRGLSSRGPDAGCLTEEVEEPAPTERPAQPGAANPLVVGAVALLDLSLAFLFSQLLT

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC052049
ORF Size 1884 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC052049.1
RefSeq Size 3556 bp
RefSeq ORF 1886 bp
Locus ID 319984
Cytogenetics 14 C3
Summary Junctophilins contribute to the formation of junctional membrane complexes (JMCs) which link the plasma membrane with the endoplasmic or sarcoplasmic reticulum in excitable cells. Provides a structural foundation for functional cross-talk between the cell surface and intracellular calcium release channels. JPH4 is brain-specific and appears to have an active role in certain neurons involved in motor coordination and memory.UniProtKB/Swiss-Prot Function
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Resources
"BC052049" in other vectors (4)
SKU Description Size Price
MC218681 Jph4 (untagged) - Mouse junctophilin 4 (cDNA clone MGC:62430 IMAGE:5689347), (10ug) 10 ug
$644.00
MR209581 Jph4 (Myc-DDK-tagged) - Mouse junctophilin 4 (cDNA clone MGC:62430 IMAGE:5689347) 10 ug
$289.00 $585.00
MR209581L3 Lenti ORF clone of Jph4 (Myc-DDK-tagged) - Mouse junctophilin 4 (cDNA clone MGC:62430 IMAGE:5689347) 10 ug
$885.00
MR209581L4 Lenti ORF clone of Jph4 (mGFP-tagged) - Mouse junctophilin 4 (cDNA clone MGC:62430 IMAGE:5689347) 10 ug
$885.00

Plasmid Map for MG209581

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG209581
Circular map for MG209581

file-alt Citations

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