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Gpaa1 (NM_010331) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MG209501
Gpaa1 (tGFP-tagged) - Mouse GPI anchor attachment protein 1 (Gpaa1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
tGFP DDK
AAV Particle:
DDK
  $779.00
3 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gpaa1
Synonyms C80044; mGAA1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG209501 representing NM_010331
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTCTCCTGTCGGACCCGGTGCGCCGGCGCGCGCTCGCCCGCATCGTCTTGCGCCTTAACACACCTC
TCTGTGTGCTGAGCTACGTGGCCGGGATCGCCTGGTTTCTGGCGCTGGCTTTTCCGCCGTTAACCCAGCG
CACTTACATGTCTGAGAATGCCATGGGCTCCACCATGGTGGAGGAACAGTTTGTAGGTGGAGACCGCGCT
CGGAGCTTTGCCCGGGACTTCGCTGCCCACCGCAAGAAGCCGGGGGCTCTGCCAGTAGCCTGGCTGGAAC
GGTCCATGCGATCCGTAGGGCTGGAGGTCTACACCCAGAGCTTCTCCCGCAAACTACCCTTCCCGGATGA
GACCCACGAGCGCTATATGGTGTCAGGTACCAACGTGTATGGCATCCTGCGGGCCCCACGTTCTGCCAGT
ACCGAGTCCCTGGTGCTTACTGTACCCTGTGGTCCAGATGCCACCAATAGCCAGGCTGTGGGGTTGTTGT
TGGCACTTGCTGCCCATTTCCGAGGGCAGATTTACTGGGCCAAAGATATCATTTTCCTGGTAACAGACCA
TGATCTTCTGGGCACCGAGGCTTGGCTCGAAGCCTACCATGACATTAATGTTACAGGCATACAGTCATCT
CCCCTGCAGGGCAGGGCTGGAGCCATCCAGGCTGCTGTAGCTCTGGAGCTGAGCAGCGACGTGGTCACCA
GCCTCGATGTGACAGTGGAGGGACTCAACGGTCAGCTGCCCAACCTTGATCTGCTCAACCTCTTCCAGAC
CTTCTGCCAGAAAGGGGGGCTGTTGTGTACACTCCAGGGCAAGCTGCAGCCCCAGGACTGGACGTCACTC
GAAGGGCCGTTGCAGGGTTTGCAGACACTGCTACTCATGGTGCTGCGGCAGGCCTCTGGCCGGCCCCACG
GCCCCCACGGCCTTTTTCTGCGCTACGGAGTGGAGGCACTAACCCTACGCGGCATCAATAGCTTCCGCCA
ATACAAGTATGATTTGGCGACAGTGGGCAAGGCTCTGGAGGGCATGTTCCGCAAGCTCAACCACCTCCTG
GAGCGCCTGCACCAGTCATTCTTCTTCTACCTGCTGCCGGCCCTCTCCCGCTTTGTCTCCATCGGACTCT
ACATGCCCGCCACGGGCTTCCTGCTCCTAGTCCTTGGACTCAAGGCTCTGGAGCTGTGGATGCAGTTACA
CCAGGCTGGAGTCAACCCGGAGGAGGCTGGGAAGGCCCCTAGTCCCGGGACTCCACTCTTGCCAACACAG
GGTGTGGGCCTGGCCTCACTTACGGCACCCCTGCTGATCTCTCAGGCCATGGGTCTGGCCCTCTATTTCC
TGCCTGTGCTGGGCCAACATTTAGCTACTCAGCATTTCCCAGTGGCCGAAGCCGAGGCTGTTGTGTTGAC
GCTGCTCGCGATTTATGTAGCCGGCCTGGCCCTTCCACACAACACCCACCGAGTGGTCAACTCACAGGTC
CCAGACAGGGGCTGGATGGCACTGAAACTAGTGGCCCTCATCTACCTGGCACTGCAGCTGGGTTGCATTG
CTCTCCTCAATTTCTCCCTGGGCTTCCTGCTGGCTGCCACCATGGTCCCTGCTGCTGCACTCGCCAAGCC
CCATGGGCCCCGGACACTGTATGCTGCCCTGCTGGTAGTGACCAGCCCAGCAGTCACACTCTTCGGTAGC
CTGTTCCTGTGGCGGGAGCTGCTGGAGGTACCGCTGTCTCTGGCAGAGGGTTGGCAGCTCTTCCTCACAG
CATTGGCCCAGGGTGTGCTGGAACACTACACCTATGGCGCCCTCCTCTTCCCAATACTGGCCTTGGGTCT
GTATCCTTGCTGGCTGCTTTTCTGGAATGTCCTCTTCTGGAAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG209501 representing NM_010331
Red=Cloning site Green=Tags(s)

MGLLSDPVRRRALARIVLRLNTPLCVLSYVAGIAWFLALAFPPLTQRTYMSENAMGSTMVEEQFVGGDRA
RSFARDFAAHRKKPGALPVAWLERSMRSVGLEVYTQSFSRKLPFPDETHERYMVSGTNVYGILRAPRSAS
TESLVLTVPCGPDATNSQAVGLLLALAAHFRGQIYWAKDIIFLVTDHDLLGTEAWLEAYHDINVTGIQSS
PLQGRAGAIQAAVALELSSDVVTSLDVTVEGLNGQLPNLDLLNLFQTFCQKGGLLCTLQGKLQPQDWTSL
EGPLQGLQTLLLMVLRQASGRPHGPHGLFLRYGVEALTLRGINSFRQYKYDLATVGKALEGMFRKLNHLL
ERLHQSFFFYLLPALSRFVSIGLYMPATGFLLLVLGLKALELWMQLHQAGVNPEEAGKAPSPGTPLLPTQ
GVGLASLTAPLLISQAMGLALYFLPVLGQHLATQHFPVAEAEAVVLTLLAIYVAGLALPHNTHRVVNSQV
PDRGWMALKLVALIYLALQLGCIALLNFSLGFLLAATMVPAAALAKPHGPRTLYAALLVVTSPAVTLFGS
LFLWRELLEVPLSLAEGWQLFLTALAQGVLEHYTYGALLFPILALGLYPCWLLFWNVLFWK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010331
ORF Size 1863 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010331.2, NP_034461.1
RefSeq Size 2036 bp
RefSeq ORF 1866 bp
Locus ID 14731
UniProt ID Q9WTK3
Cytogenetics 15 D3
Summary Essential for GPI-anchoring of precursor proteins but not for GPI synthesis. Acts before or during formation of the carbonyl intermediate.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_010331" in other vectors (4)
SKU Description Size Price
MC200605 Gpaa1 (untagged) - Mouse GPI anchor attachment protein 1 (Gpaa1), (10ug) 10 ug
$579.00
MR209501 Gpaa1 (Myc-DDK-tagged) - Mouse GPI anchor attachment protein 1 (Gpaa1) 10 ug
$289.00 $579.00
MR209501L3 Lenti ORF clone of Gpaa1 (Myc-DDK-tagged) - Mouse GPI anchor attachment protein 1 (Gpaa1) 10 ug
$879.00
MR209501L4 Lenti ORF clone of Gpaa1 (mGFP-tagged) - Mouse GPI anchor attachment protein 1 (Gpaa1) 10 ug
$879.00

Plasmid Map for MG209501

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MG209501
Circular map for MG209501

file-alt Citations

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