Ermap (NM_013848) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MG209226
Ermap (tGFP-tagged) - Mouse erythroblast membrane-associated protein (Ermap)
  $1,025.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ermap
Synonyms AA409279; AI666418
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MG209226 representing NM_013848
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGAAGCGGCTAAAGAAACATGTGGTGGCGTGGAAAATGTGTGTAATGCCACACTCACGGAAGATGT
CAGTTCATATGGAGAGACCAAGTCCCTGTGGTTCCTGGCTTGTCGGCTGTCTCTTCACCATAGCTGTCTT
CCAGCCGCCTGTTCAGGTGTTAGGGGATGCTGGCAAGGTCTACATAGCCCCTCTTAGGGACACAGCCAAC
TTGCCCTGCCCTCTCTTCCTCTGGCCTAACATGGTACTCAGCGAGATGAGGTGGTATCGGCCCGGACACC
TACCTCGCACCCAGGCTGTCCACGTGTTCCGGGACGGGCAGGACAGAGATGAAGACCTGATGCCAGAATA
TAAGGGCAGGACGGCACTGGTGAGGGATGCCCACAAGGAAAGCTACATCCTGCAAATCAGTAATGTGAGG
CTCGAGGACCGAGGGCTATACCAGTGCCAGGTCTGGGTCGGAAACTCAAGTCGAGAAGACAACGTGACCC
TGCAGGTGGCAGGTTCTGATCCCTACATCCACGTGAAGGGCTATGATGCTGGGTGGATCGAGCTGCTGTG
TCAATCAGTGGGATGGTTCCCAAAGCCGTGGACTGAGTGGAGAGACACTACGGGTAGAGCGCTACTCTCC
CTTTCAGAGGTTCACTCCCTGGATGAAAATGGGCTGTTCCGAACAGCAGTGTCCAGCAGAATCAGGGACA
ATGCCCTGGGAAATGTGTCTTGCACTATCCATAATGAGGCCCTTGGCCAAGAGAAGACGACGGCCATGAT
TATTGGAGCCCCAGAACGAGGAAGTCTGTCCTCTCCAGCGGTGGCTCTTTCTGTGGTCTTGCCCGTCTTG
GGGCTCCTCATCCTCCTTGGCATCTGGCTCATTTGTAAACAAAAAAAATCAAAAGAGAAGCTGCTGTATG
AGCAAGCGATGGAGGTAGAAAATCTTCTGGAAGACCACGCCAAAGAAAAAGGAAGACTCCACAAAGCCCT
CAAGAAACTCCGGAGTGAACTGAAGTTGAAAAGAGCGGCGGCCAATGCAGGCTGGAGAAGAGCCCGGCTA
CATTTTGTGGCAGTGACCCTGGACCCTGACACGGCACATCCCAAACTCATCCTGTCTGAGGATCGGAGAT
GTGTGAGGCTCGGCGACAGGAAGCGGCCTGTTCCTGACAACCCAGAGAGGTTTGATTTTGTTGTCAGCGT
CCTGGGCTCCGAGTATTTCACAACCGGCTGTCACTACTGGGAAGTGTACGTGGGCGAGAAGACCAAATGG
ATTCTTGGAGTGTGTAGTGAGTCTGTGAGCAGGAAAGGGAAGGTCACGGCCTCGCCTGCCAATGGCCACT
GGCTTGTGCGACAGAGCCGTGGGAATGAGTATGAAGCTCTCACGTCCCCACAGACCTCCTTCCGCCTCAA
AGAGTCCCCGAAGTGTGTGGGGATTTTCCTGGACTATGAAGCCGGCATCATCTCCTTCTACAACGTGACT
GATAAGTCCCACATCTTCACTTTCACCCACAGTTTCTCGAGCCCACTTCGCCCTTTCTTTGAACCTTGTC
TTCACGATGAGGGGAAAAACACGGCCCCTCTAATCATTTGTACTGAGCTTCAGAAATCAGAGGAATCGAT
TGTCCCCAAACAAGAAGGAAAAGACCGTGCTAATGGAGACGTGTCCCTGAAGATGAACCCATCCTTACTG
TCCCCTCAGGGTTCTGAGCTATTTCTGCTTAATGATACCTGGCCCTCTAACCTTGGCCCTGCCCTTAAGG
GCCTCAAAGTTCCTTCTTTG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>MG209226 representing NM_013848
Red=Cloning site Green=Tags(s)

MLKRLKKHVVAWKMCVMPHSRKMSVHMERPSPCGSWLVGCLFTIAVFQPPVQVLGDAGKVYIAPLRDTAN
LPCPLFLWPNMVLSEMRWYRPGHLPRTQAVHVFRDGQDRDEDLMPEYKGRTALVRDAHKESYILQISNVR
LEDRGLYQCQVWVGNSSREDNVTLQVAGSDPYIHVKGYDAGWIELLCQSVGWFPKPWTEWRDTTGRALLS
LSEVHSLDENGLFRTAVSSRIRDNALGNVSCTIHNEALGQEKTTAMIIGAPERGSLSSPAVALSVVLPVL
GLLILLGIWLICKQKKSKEKLLYEQAMEVENLLEDHAKEKGRLHKALKKLRSELKLKRAAANAGWRRARL
HFVAVTLDPDTAHPKLILSEDRRCVRLGDRKRPVPDNPERFDFVVSVLGSEYFTTGCHYWEVYVGEKTKW
ILGVCSESVSRKGKVTASPANGHWLVRQSRGNEYEALTSPQTSFRLKESPKCVGIFLDYEAGIISFYNVT
DKSHIFTFTHSFSSPLRPFFEPCLHDEGKNTAPLIICTELQKSEESIVPKQEGKDRANGDVSLKMNPSLL
SPQGSELFLLNDTWPSNLGPALKGLKVPSL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013848
ORF Size 1770 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013848.1, NP_038876.1
RefSeq Size 4573 bp
RefSeq ORF 1779 bp
Locus ID 27028
UniProt ID Q9JLN5
Cytogenetics 4 55.34 cM
Summary Possible role as a cell-adhesion or receptor molecule of erythroid cells.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_013848" in other vectors (4)
SKU Description Size Price
MC219367 Ermap (untagged) - Mouse erythroblast membrane-associated protein (Ermap), (10ug) 10 ug
$883.00
MR209226 Ermap (Myc-DDK-tagged) - Mouse erythroblast membrane-associated protein (Ermap) 10 ug
$825.00
MR209226L3 Lenti ORF clone of Ermap (Myc-DDK-tagged) - Mouse erythroblast membrane-associated protein (Ermap) 10 ug
$1,125.00
MR209226L4 Lenti ORF clone of Ermap (mGFP-tagged) - Mouse erythroblast membrane-associated protein (Ermap) 10 ug
$1,125.00

Plasmid Map for MG209226

Download sequence for

Cloning scheme for MG209226

Circular map for MG209226

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